Characterization of a recombinant sucrose isomerase and its application to enzymatic production of isomaltulose View Full Text


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Article Info

DATE

2020-09-10

AUTHORS

Feng Zhang, Feng Cheng, Dong-Xu Jia, Yue-Hao Gu, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

ObjectiveTo characterize a recombinant isomerase that can catalyze the isomerization of sucrose into isomaltulose and investigate its application for the enzymatic production of isomaltulose.ResultsA sucrose isomerase gene from Erwinia sp. Ejp617 was synthesized and expressed in Escherichia coli BL21(DE3). The enzymatic characterization revealed that the optimal pH and temperature of the purified sucrose isomerase were 6.0 and 40 °C, respectively. The enzyme activity was slightly activated by Mn2+and Mg2+, but partially inhibited by Ca2+, Ba2+, Cu2+, Zn2+ and EDTA. The kinetic parameters of Km and Vmax for sucrose were 69.28 mM and 118.87 U/mg, respectively. The time course showed that 240.9 g/L of isomaltulose was produced from 300 g/L of sucrose, and the yield reached 80.3% after bioreaction for 180 min.ConclusionsThis recombinant enzyme showed excellent capability for biotransforming sucrose to isomaltulose at the substrate concentration of 300 g/L. Further investigations should be carried out focusing on selection of suitable heterologous expression system with the aim to improve its expression level. More... »

PAGES

261-269

References to SciGraph publications

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  • 2014-05-28. Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2010-06-30. Cloning and Characterization of a Sucrose Isomerase from Erwinia rhapontici NX-5 for Isomaltulose Hyperproduction in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2013-01-09. Enhancing isomaltulose production by recombinant Escherichia coli producing sucrose isomerase: culture medium optimization containing agricultural wastes and cell immobilization in BIOPROCESS AND BIOSYSTEMS ENGINEERING
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  • 2015-06-16. Improvement and characterization of a hyperthermophilic glucose isomerase from Thermoanaerobacterethanolicus and its application in production of high fructose corn syrup in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2019-09-24. Economical production of isomaltulose from agricultural residues in a system with sucrose isomerase displayed on Bacillus subtilis spores in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • 2006-12-08. Conversion of sucrose into isomaltulose by Enterobacter sp. FMB1, an isomaltulose-producing microorganism isolated from traditional Korean food in BIOTECHNOLOGY LETTERS
  • 2019-04-17. Display of a sucrose isomerase on the cell surface of Yarrowia lipolytica for synthesis of isomaltulose from sugar cane by-products in 3 BIOTECH
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    http://scigraph.springernature.com/pub.10.1007/s10529-020-02999-7

    DOI

    http://dx.doi.org/10.1007/s10529-020-02999-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1130761595

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32910357


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