Enhanced production of l-methionine in engineered Escherichia coli with efficient supply of one carbon unit View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-12-21

AUTHORS

Xiao-Ling Tang, Xu-Yuan Du, Lu-Jia Chen, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

Objectivel-methionine is an important sulfur-containing amino acid essential for humans and animals. Its biosynthesis pathway is complex and highly regulated. This study aims to explore the bottleneck limiting the improvement of l-methionine productivity and apply efficient strategies to increase l-methionine production in engineered E. coli.ResultsThe enzyme O-succinylhomoserine sulfhydrylase involved in thiolation of OSH to form homocysteine was overexpressed in the engineered strain E. coli W3110 IJAHFEBC/PAm, resulting in l-methionine production increased from 2.8 to 3.22 g/L in shake flask cultivation. By exogenous addition of l-glycine as the precursor of one carbon unit, the titer of l-methionine was increased to 3.68 g/L. The glycine cleavage system was further strengthened for the efficient one carbon unit supply and a l-methionine titer of 3.96 g/L was obtained, which was increased by 42% compared with that of the original strain.ConclusionsInsufficient supply of one carbon unit was found to be the issue limiting the improvement of l-methionine productivity and its up-regulation significantly promoted the l-methionine production in the engineered E. coli. More... »

PAGES

429-436

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-019-02786-z

DOI

http://dx.doi.org/10.1007/s10529-019-02786-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1123593973

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31865476


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