A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-11

AUTHORS

Gayle F. Petersen, Bryan J. Hilbert, Gareth D. Trope, Wouter H. J. Kalle, Padraig M. Strappe

ABSTRACT

OBJECTIVES: We investigated the applicability of single layer paper-based scaffolds for the three-dimensional (3D) growth and osteogenic differentiation of equine adipose-derived stem cells (EADSC), with comparison against conventional two-dimensional (2D) culture on polystyrene tissue culture vessels. RESULTS: Viable culture of EADSC was achieved using paper-based scaffolds, with EADSC grown and differentiated in 3D culture retaining high cell viability (>94 %), similarly to EADSC in 2D culture. Osteogenic differentiation of EADSC was significantly enhanced in 3D culture, with Alizarin Red S staining and quantification demonstrating increased mineralisation (p < 0.0001), and an associated increase in expression of the osteogenic-specific markers alkaline phosphatase (p < 0.0001), osteopontin (p < 0.0001), and runx2 (p < 0.01). Furthermore, scanning electron microscopy revealed a spherical morphology of EADSC in 3D culture, compared to a flat morphology of EADSC in 2D culture. CONCLUSIONS: Single layer paper-based scaffolds provide an enhanced environment for the in vitro 3D growth and osteogenic differentiation of EADSC, with high cell viability, and a spherical morphology. More... »

PAGES

2321-2331

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-015-1898-x

DOI

http://dx.doi.org/10.1007/s10529-015-1898-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005128726

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26140902


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