Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-07

AUTHORS

Saliya Gurusinghe, Peter Young, Jacob Michelsen, Padraig Strappe

ABSTRACT

OBJECTIVES: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects. RESULTS: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM). 3D pellet cultures of transduced cells in the presence of TGFβ-3 revealed increased pellet size and higher levels of total glycosaminoglycan in both Sox9 and Sox9+ OSKM co-transduced chondrocytes compared to untransduced and green fluorescent protein expressing controls. Immunohistochemical detection of Sox9 and collagen II was evident in transduced cells (Sox9, OSKM, or Sox9+ OSKM) with very low levels in untransduced chondrocytes, demonstrating a dedifferentiated state (P < 0.01). The marker for chondrocyte hypertrophy, collagen X was highly expressed in Sox9 transduced chondrocytes but lower in OSKM or Sox9+ OSKM cells (P < 0.05). CONCLUSION: A combination of Sox9 and OSKM gene delivery to canine chondrocytes allows continuous proliferation in monolayer culture with a higher expression of col2a1 without an increase in the hypertrophy marker collagen X in 3D pellet cultures. More... »

PAGES

1495-1504

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5

DOI

http://dx.doi.org/10.1007/s10529-015-1805-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034813768

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25813774


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Dedifferentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Enlargement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chondrocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dogs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lentivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "SOX9 Transcription Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gurusinghe", 
        "givenName": "Saliya", 
        "id": "sg:person.01200356350.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200356350.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Young", 
        "givenName": "Peter", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Michelsen", 
        "givenName": "Jacob", 
        "id": "sg:person.01336263356.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336263356.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia", 
            "Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Strappe", 
        "givenName": "Padraig", 
        "id": "sg:person.01015736676.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015736676.90"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.joca.2004.10.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000430028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0077365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001679209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2011.137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003363495", 
          "https://doi.org/10.1038/cdd.2011.137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jor.20523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005195862"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.24388", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005256675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11427-011-4179-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007516003", 
          "https://doi.org/10.1007/s11427-011-4179-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10529-010-0514-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008222869", 
          "https://doi.org/10.1007/s10529-010-0514-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010904856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(94)80292-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012831262"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(02)03026-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013665217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(02)03026-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013665217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.20611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016782209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-011-9239-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020262768", 
          "https://doi.org/10.1007/s12015-011-9239-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-011-9239-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020262768", 
          "https://doi.org/10.1007/s12015-011-9239-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.076992", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022161308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-014-9538-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022550684", 
          "https://doi.org/10.1007/s12015-014-9538-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-014-9538-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022550684", 
          "https://doi.org/10.1007/s12015-014-9538-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2010.03.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022874771"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0022008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025307841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci44605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026708846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2011.06.194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029044921"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12265-010-9233-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030593249", 
          "https://doi.org/10.1007/s12265-010-9233-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/0471142301.nsa01cs01", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032097665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.150540", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034657805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.08.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036662790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.08.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036662790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2012/429160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038504947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2013.01.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042240293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201308102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045700279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199410063311401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046886095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m413913200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053042379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/0264-6021:3600461", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056715880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/107632704323061933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059206402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.2004.15.976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059272421"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2008.0335", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059309915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2527/jas.2010-3666", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070887719"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-07", 
    "datePublishedReg": "2015-07-01", 
    "description": "OBJECTIVES: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects.\nRESULTS: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM). 3D pellet cultures of transduced cells in the presence of TGF\u03b2-3 revealed increased pellet size and higher levels of total glycosaminoglycan in both Sox9 and Sox9+ OSKM co-transduced chondrocytes compared to untransduced and green fluorescent protein expressing controls. Immunohistochemical detection of Sox9 and collagen II was evident in transduced cells (Sox9, OSKM, or Sox9+ OSKM) with very low levels in untransduced chondrocytes, demonstrating a dedifferentiated state (P < 0.01). The marker for chondrocyte hypertrophy, collagen X was highly expressed in Sox9 transduced chondrocytes but lower in OSKM or Sox9+ OSKM cells (P < 0.05).\nCONCLUSION: A combination of Sox9 and OSKM gene delivery to canine chondrocytes allows continuous proliferation in monolayer culture with a higher expression of col2a1 without an increase in the hypertrophy marker collagen X in 3D pellet cultures.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10529-015-1805-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "37"
      }
    ], 
    "name": "Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors", 
    "pagination": "1495-1504", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a5d6e401449d01c319d2d4c8e7726529fc82b1e6d26c98e8ddbfdf92971310f9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25813774"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8008051"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10529-015-1805-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034813768"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10529-015-1805-5", 
      "https://app.dimensions.ai/details/publication/pub.1034813768"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs10529-015-1805-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'


 

This table displays all metadata directly associated to this object as RDF triples.

240 TRIPLES      21 PREDICATES      73 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10529-015-1805-5 schema:about N045b30387c27416385a7b2abf1eb66b0
2 N0789be791c5d413c874c89a07a2a3383
3 N24cc94af38b0497bb9bead77019fbd09
4 N410f0551734f47dc974f760013fefc45
5 N44e269ccddfd4653b57d82942124e7ef
6 N5ba08ab79df945a19afd63faf9e9bfff
7 N7d92b5d630be445c828999de50667e56
8 N8f1fbd89b45d49d9926bc0d26ac6ab47
9 N9d4a53c6a3814de7b8aa1ebf9ad72943
10 Nb9f5dcc5fd2341e29163b9d349819cc6
11 Ne3951c3bee6a4a7db15a816b2e849887
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author N68b769f0d4f44e54bf64363a74b965d9
15 schema:citation sg:pub.10.1007/s10529-010-0514-3
16 sg:pub.10.1007/s11427-011-4179-5
17 sg:pub.10.1007/s12015-011-9239-5
18 sg:pub.10.1007/s12015-014-9538-8
19 sg:pub.10.1007/s12265-010-9233-3
20 sg:pub.10.1038/cdd.2011.137
21 https://doi.org/10.1002/0471142301.nsa01cs01
22 https://doi.org/10.1002/art.20611
23 https://doi.org/10.1002/jcb.24388
24 https://doi.org/10.1002/jor.20523
25 https://doi.org/10.1016/0014-5793(94)80292-0
26 https://doi.org/10.1016/j.bbrc.2011.06.194
27 https://doi.org/10.1016/j.cell.2006.07.024
28 https://doi.org/10.1016/j.cell.2007.11.019
29 https://doi.org/10.1016/j.cell.2010.03.030
30 https://doi.org/10.1016/j.cell.2012.08.033
31 https://doi.org/10.1016/j.joca.2004.10.011
32 https://doi.org/10.1016/j.joca.2013.01.014
33 https://doi.org/10.1016/s0006-291x(02)03026-7
34 https://doi.org/10.1042/0264-6021:3600461
35 https://doi.org/10.1056/nejm199410063311401
36 https://doi.org/10.1074/jbc.m109.076992
37 https://doi.org/10.1074/jbc.m110.150540
38 https://doi.org/10.1074/jbc.m413913200
39 https://doi.org/10.1083/jcb.201308102
40 https://doi.org/10.1089/107632704323061933
41 https://doi.org/10.1089/hum.2004.15.976
42 https://doi.org/10.1089/scd.2008.0335
43 https://doi.org/10.1155/2012/429160
44 https://doi.org/10.1172/jci44605
45 https://doi.org/10.1371/journal.pone.0022008
46 https://doi.org/10.1371/journal.pone.0077365
47 https://doi.org/10.2527/jas.2010-3666
48 schema:datePublished 2015-07
49 schema:datePublishedReg 2015-07-01
50 schema:description OBJECTIVES: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects. RESULTS: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM). 3D pellet cultures of transduced cells in the presence of TGFβ-3 revealed increased pellet size and higher levels of total glycosaminoglycan in both Sox9 and Sox9+ OSKM co-transduced chondrocytes compared to untransduced and green fluorescent protein expressing controls. Immunohistochemical detection of Sox9 and collagen II was evident in transduced cells (Sox9, OSKM, or Sox9+ OSKM) with very low levels in untransduced chondrocytes, demonstrating a dedifferentiated state (P < 0.01). The marker for chondrocyte hypertrophy, collagen X was highly expressed in Sox9 transduced chondrocytes but lower in OSKM or Sox9+ OSKM cells (P < 0.05). CONCLUSION: A combination of Sox9 and OSKM gene delivery to canine chondrocytes allows continuous proliferation in monolayer culture with a higher expression of col2a1 without an increase in the hypertrophy marker collagen X in 3D pellet cultures.
51 schema:genre research_article
52 schema:inLanguage en
53 schema:isAccessibleForFree false
54 schema:isPartOf N06b2f4710fc0471991c0a75cb302767c
55 Nf132c7866dd945d3840fbd153d5524ce
56 sg:journal.1289037
57 schema:name Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors
58 schema:pagination 1495-1504
59 schema:productId N32b34aa98d584908a5a8e8494f3d1ae2
60 N34e4f7fcc27c40f893c115b4c2b5799c
61 N94a4257926c246b4ad7e5f01b48f7820
62 Naa8266105f77451cac1b36ea8e24a39d
63 Nd4872d06546e480eb51967b2815a7062
64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034813768
65 https://doi.org/10.1007/s10529-015-1805-5
66 schema:sdDatePublished 2019-04-10T15:01
67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
68 schema:sdPublisher N5398fe7810574c509b2c09d7f20f2e25
69 schema:url http://link.springer.com/10.1007%2Fs10529-015-1805-5
70 sgo:license sg:explorer/license/
71 sgo:sdDataset articles
72 rdf:type schema:ScholarlyArticle
73 N045b30387c27416385a7b2abf1eb66b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Dogs
75 rdf:type schema:DefinedTerm
76 N06b2f4710fc0471991c0a75cb302767c schema:volumeNumber 37
77 rdf:type schema:PublicationVolume
78 N0789be791c5d413c874c89a07a2a3383 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Cell Dedifferentiation
80 rdf:type schema:DefinedTerm
81 N24cc94af38b0497bb9bead77019fbd09 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name SOX9 Transcription Factor
83 rdf:type schema:DefinedTerm
84 N32b34aa98d584908a5a8e8494f3d1ae2 schema:name pubmed_id
85 schema:value 25813774
86 rdf:type schema:PropertyValue
87 N34e4f7fcc27c40f893c115b4c2b5799c schema:name readcube_id
88 schema:value a5d6e401449d01c319d2d4c8e7726529fc82b1e6d26c98e8ddbfdf92971310f9
89 rdf:type schema:PropertyValue
90 N410f0551734f47dc974f760013fefc45 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Male
92 rdf:type schema:DefinedTerm
93 N44e269ccddfd4653b57d82942124e7ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Transcription Factors
95 rdf:type schema:DefinedTerm
96 N53982df8b48246f7ad41f6672f6790a2 rdf:first sg:person.01015736676.90
97 rdf:rest rdf:nil
98 N5398fe7810574c509b2c09d7f20f2e25 schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N5ba08ab79df945a19afd63faf9e9bfff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Gene Expression Profiling
102 rdf:type schema:DefinedTerm
103 N68b769f0d4f44e54bf64363a74b965d9 rdf:first sg:person.01200356350.18
104 rdf:rest Nf126c281a3e147bab5e7b657331b6579
105 N7d92b5d630be445c828999de50667e56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Chondrocytes
107 rdf:type schema:DefinedTerm
108 N8f1fbd89b45d49d9926bc0d26ac6ab47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Animals
110 rdf:type schema:DefinedTerm
111 N94a4257926c246b4ad7e5f01b48f7820 schema:name nlm_unique_id
112 schema:value 8008051
113 rdf:type schema:PropertyValue
114 N9d4a53c6a3814de7b8aa1ebf9ad72943 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Cells, Cultured
116 rdf:type schema:DefinedTerm
117 N9fea24de2b724402b20bf8054b2d2a3b schema:affiliation https://www.grid.ac/institutes/grid.1037.5
118 schema:familyName Young
119 schema:givenName Peter
120 rdf:type schema:Person
121 Naa8266105f77451cac1b36ea8e24a39d schema:name dimensions_id
122 schema:value pub.1034813768
123 rdf:type schema:PropertyValue
124 Nb9f5dcc5fd2341e29163b9d349819cc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Lentivirus
126 rdf:type schema:DefinedTerm
127 Nd4872d06546e480eb51967b2815a7062 schema:name doi
128 schema:value 10.1007/s10529-015-1805-5
129 rdf:type schema:PropertyValue
130 Ne3951c3bee6a4a7db15a816b2e849887 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Cell Enlargement
132 rdf:type schema:DefinedTerm
133 Nf126c281a3e147bab5e7b657331b6579 rdf:first N9fea24de2b724402b20bf8054b2d2a3b
134 rdf:rest Nf40752973e74473883030d2f4adea7b2
135 Nf132c7866dd945d3840fbd153d5524ce schema:issueNumber 7
136 rdf:type schema:PublicationIssue
137 Nf40752973e74473883030d2f4adea7b2 rdf:first sg:person.01336263356.06
138 rdf:rest N53982df8b48246f7ad41f6672f6790a2
139 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
140 schema:name Biological Sciences
141 rdf:type schema:DefinedTerm
142 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biochemistry and Cell Biology
144 rdf:type schema:DefinedTerm
145 sg:journal.1289037 schema:issn 0951-208X
146 1573-6784
147 schema:name Biotechnology Letters
148 rdf:type schema:Periodical
149 sg:person.01015736676.90 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
150 schema:familyName Strappe
151 schema:givenName Padraig
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015736676.90
153 rdf:type schema:Person
154 sg:person.01200356350.18 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
155 schema:familyName Gurusinghe
156 schema:givenName Saliya
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200356350.18
158 rdf:type schema:Person
159 sg:person.01336263356.06 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
160 schema:familyName Michelsen
161 schema:givenName Jacob
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336263356.06
163 rdf:type schema:Person
164 sg:pub.10.1007/s10529-010-0514-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008222869
165 https://doi.org/10.1007/s10529-010-0514-3
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/s11427-011-4179-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007516003
168 https://doi.org/10.1007/s11427-011-4179-5
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/s12015-011-9239-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020262768
171 https://doi.org/10.1007/s12015-011-9239-5
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s12015-014-9538-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022550684
174 https://doi.org/10.1007/s12015-014-9538-8
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s12265-010-9233-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030593249
177 https://doi.org/10.1007/s12265-010-9233-3
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/cdd.2011.137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003363495
180 https://doi.org/10.1038/cdd.2011.137
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1002/0471142301.nsa01cs01 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032097665
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1002/art.20611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016782209
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1002/jcb.24388 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005256675
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1002/jor.20523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005195862
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/0014-5793(94)80292-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012831262
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.bbrc.2011.06.194 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029044921
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.cell.2010.03.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022874771
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.cell.2012.08.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036662790
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.joca.2004.10.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000430028
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/j.joca.2013.01.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042240293
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/s0006-291x(02)03026-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013665217
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1042/0264-6021:3600461 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056715880
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1056/nejm199410063311401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046886095
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1074/jbc.m109.076992 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022161308
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1074/jbc.m110.150540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034657805
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1074/jbc.m413913200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053042379
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1083/jcb.201308102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045700279
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1089/107632704323061933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059206402
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1089/hum.2004.15.976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272421
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1089/scd.2008.0335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059309915
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1155/2012/429160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038504947
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1172/jci44605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026708846
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1371/journal.pone.0022008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025307841
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1371/journal.pone.0077365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001679209
233 rdf:type schema:CreativeWork
234 https://doi.org/10.2527/jas.2010-3666 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070887719
235 rdf:type schema:CreativeWork
236 https://www.grid.ac/institutes/grid.1037.5 schema:alternateName Charles Sturt University
237 schema:name Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW, Australia
238 School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia
239 School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia
240 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...