Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-07

AUTHORS

Saliya Gurusinghe, Peter Young, Jacob Michelsen, Padraig Strappe

ABSTRACT

OBJECTIVES: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects. RESULTS: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM). 3D pellet cultures of transduced cells in the presence of TGFβ-3 revealed increased pellet size and higher levels of total glycosaminoglycan in both Sox9 and Sox9+ OSKM co-transduced chondrocytes compared to untransduced and green fluorescent protein expressing controls. Immunohistochemical detection of Sox9 and collagen II was evident in transduced cells (Sox9, OSKM, or Sox9+ OSKM) with very low levels in untransduced chondrocytes, demonstrating a dedifferentiated state (P < 0.01). The marker for chondrocyte hypertrophy, collagen X was highly expressed in Sox9 transduced chondrocytes but lower in OSKM or Sox9+ OSKM cells (P < 0.05). CONCLUSION: A combination of Sox9 and OSKM gene delivery to canine chondrocytes allows continuous proliferation in monolayer culture with a higher expression of col2a1 without an increase in the hypertrophy marker collagen X in 3D pellet cultures. More... »

PAGES

1495-1504

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5

DOI

http://dx.doi.org/10.1007/s10529-015-1805-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034813768

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25813774


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Dedifferentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Enlargement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chondrocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dogs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lentivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "SOX9 Transcription Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gurusinghe", 
        "givenName": "Saliya", 
        "id": "sg:person.01200356350.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200356350.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Young", 
        "givenName": "Peter", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Michelsen", 
        "givenName": "Jacob", 
        "id": "sg:person.01336263356.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336263356.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles Sturt University", 
          "id": "https://www.grid.ac/institutes/grid.1037.5", 
          "name": [
            "School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia", 
            "Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Strappe", 
        "givenName": "Padraig", 
        "id": "sg:person.01015736676.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015736676.90"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.joca.2004.10.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000430028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0077365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001679209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2011.137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003363495", 
          "https://doi.org/10.1038/cdd.2011.137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jor.20523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005195862"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.24388", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005256675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11427-011-4179-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007516003", 
          "https://doi.org/10.1007/s11427-011-4179-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10529-010-0514-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008222869", 
          "https://doi.org/10.1007/s10529-010-0514-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010904856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(94)80292-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012831262"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(02)03026-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013665217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(02)03026-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013665217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.20611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016782209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-011-9239-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020262768", 
          "https://doi.org/10.1007/s12015-011-9239-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-011-9239-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020262768", 
          "https://doi.org/10.1007/s12015-011-9239-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.076992", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022161308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-014-9538-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022550684", 
          "https://doi.org/10.1007/s12015-014-9538-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-014-9538-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022550684", 
          "https://doi.org/10.1007/s12015-014-9538-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2010.03.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022874771"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0022008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025307841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci44605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026708846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2011.06.194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029044921"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12265-010-9233-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030593249", 
          "https://doi.org/10.1007/s12265-010-9233-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/0471142301.nsa01cs01", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032097665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.150540", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034657805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.08.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036662790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.08.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036662790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2012/429160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038504947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2013.01.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042240293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201308102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045700279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199410063311401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046886095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m413913200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053042379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/0264-6021:3600461", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056715880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/107632704323061933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059206402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.2004.15.976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059272421"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2008.0335", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059309915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2527/jas.2010-3666", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070887719"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-07", 
    "datePublishedReg": "2015-07-01", 
    "description": "OBJECTIVES: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects.\nRESULTS: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM). 3D pellet cultures of transduced cells in the presence of TGF\u03b2-3 revealed increased pellet size and higher levels of total glycosaminoglycan in both Sox9 and Sox9+ OSKM co-transduced chondrocytes compared to untransduced and green fluorescent protein expressing controls. Immunohistochemical detection of Sox9 and collagen II was evident in transduced cells (Sox9, OSKM, or Sox9+ OSKM) with very low levels in untransduced chondrocytes, demonstrating a dedifferentiated state (P < 0.01). The marker for chondrocyte hypertrophy, collagen X was highly expressed in Sox9 transduced chondrocytes but lower in OSKM or Sox9+ OSKM cells (P < 0.05).\nCONCLUSION: A combination of Sox9 and OSKM gene delivery to canine chondrocytes allows continuous proliferation in monolayer culture with a higher expression of col2a1 without an increase in the hypertrophy marker collagen X in 3D pellet cultures.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10529-015-1805-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "37"
      }
    ], 
    "name": "Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors", 
    "pagination": "1495-1504", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a5d6e401449d01c319d2d4c8e7726529fc82b1e6d26c98e8ddbfdf92971310f9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25813774"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8008051"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10529-015-1805-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034813768"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10529-015-1805-5", 
      "https://app.dimensions.ai/details/publication/pub.1034813768"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs10529-015-1805-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10529-015-1805-5'


 

This table displays all metadata directly associated to this object as RDF triples.

240 TRIPLES      21 PREDICATES      73 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10529-015-1805-5 schema:about N009768ffa0bd4a18a164e247c52817d5
2 N0a461a64402f443dbcd45b77cfe56f07
3 N1d564fceec0b455ab84dc60e4dc10212
4 N5de096ae7a5e407aaa2d82d2597600e2
5 N72ac4911c4864e07bbd70a1a9e814fa4
6 N793d7165a3484ac0bb65cd9b3069363f
7 Nb21e0b06891f44668c07c7130fa5d3de
8 Ncb00a684108c4107b77b18950a6cbd60
9 Ne5718169281f48f78d7d517454053fb7
10 Need00243f9cb4d65ae6d6efd2f680d2e
11 Nf52c37900382469e836bd71a6ab4328c
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author Ned3175a04c694a36a0959fb761db2e78
15 schema:citation sg:pub.10.1007/s10529-010-0514-3
16 sg:pub.10.1007/s11427-011-4179-5
17 sg:pub.10.1007/s12015-011-9239-5
18 sg:pub.10.1007/s12015-014-9538-8
19 sg:pub.10.1007/s12265-010-9233-3
20 sg:pub.10.1038/cdd.2011.137
21 https://doi.org/10.1002/0471142301.nsa01cs01
22 https://doi.org/10.1002/art.20611
23 https://doi.org/10.1002/jcb.24388
24 https://doi.org/10.1002/jor.20523
25 https://doi.org/10.1016/0014-5793(94)80292-0
26 https://doi.org/10.1016/j.bbrc.2011.06.194
27 https://doi.org/10.1016/j.cell.2006.07.024
28 https://doi.org/10.1016/j.cell.2007.11.019
29 https://doi.org/10.1016/j.cell.2010.03.030
30 https://doi.org/10.1016/j.cell.2012.08.033
31 https://doi.org/10.1016/j.joca.2004.10.011
32 https://doi.org/10.1016/j.joca.2013.01.014
33 https://doi.org/10.1016/s0006-291x(02)03026-7
34 https://doi.org/10.1042/0264-6021:3600461
35 https://doi.org/10.1056/nejm199410063311401
36 https://doi.org/10.1074/jbc.m109.076992
37 https://doi.org/10.1074/jbc.m110.150540
38 https://doi.org/10.1074/jbc.m413913200
39 https://doi.org/10.1083/jcb.201308102
40 https://doi.org/10.1089/107632704323061933
41 https://doi.org/10.1089/hum.2004.15.976
42 https://doi.org/10.1089/scd.2008.0335
43 https://doi.org/10.1155/2012/429160
44 https://doi.org/10.1172/jci44605
45 https://doi.org/10.1371/journal.pone.0022008
46 https://doi.org/10.1371/journal.pone.0077365
47 https://doi.org/10.2527/jas.2010-3666
48 schema:datePublished 2015-07
49 schema:datePublishedReg 2015-07-01
50 schema:description OBJECTIVES: Prolonged in vitro culture of primary articular chondrocytes results in dedifferentiation to a fibroblast-like cell with reduced expression of the Sox9 transcription factor and the extracellular matrix protein collagen II. The ability to genetically-modify chondrocytes to allow both proliferation and maintenance of an articular phenotype may provide increased numbers of appropriate cells for regeneration of large cartilage defects. RESULTS: Canine chondrocytes were expanded in monolayer culture and transduced with a lentiviral vector expressing Sox9 or in combination with a multicistronic lentiviral vector expressing the four induced pluripotency stem (iPS) cell factors, Oct4, Klf4, Sox2 and c-Myc (OSKM). 3D pellet cultures of transduced cells in the presence of TGFβ-3 revealed increased pellet size and higher levels of total glycosaminoglycan in both Sox9 and Sox9+ OSKM co-transduced chondrocytes compared to untransduced and green fluorescent protein expressing controls. Immunohistochemical detection of Sox9 and collagen II was evident in transduced cells (Sox9, OSKM, or Sox9+ OSKM) with very low levels in untransduced chondrocytes, demonstrating a dedifferentiated state (P < 0.01). The marker for chondrocyte hypertrophy, collagen X was highly expressed in Sox9 transduced chondrocytes but lower in OSKM or Sox9+ OSKM cells (P < 0.05). CONCLUSION: A combination of Sox9 and OSKM gene delivery to canine chondrocytes allows continuous proliferation in monolayer culture with a higher expression of col2a1 without an increase in the hypertrophy marker collagen X in 3D pellet cultures.
51 schema:genre research_article
52 schema:inLanguage en
53 schema:isAccessibleForFree false
54 schema:isPartOf Nd247830ffd14454a9de6fee78bf18faf
55 Ne2f4bb891c5249deb888160527d151ba
56 sg:journal.1289037
57 schema:name Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors
58 schema:pagination 1495-1504
59 schema:productId N2aacbe44025a4fbd9aad175fcffa0352
60 N2e95e193d39c4ffba5cdc61d09ec21ef
61 N549a724873b4472cae3616b86f9d9046
62 N8a87d1f1c664432c9293e0fb33eeb316
63 Nfdcaf7a9582442d7b61b9de8fa9cd916
64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034813768
65 https://doi.org/10.1007/s10529-015-1805-5
66 schema:sdDatePublished 2019-04-10T15:01
67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
68 schema:sdPublisher N5e92305f97c045459e8849f34fd39fe1
69 schema:url http://link.springer.com/10.1007%2Fs10529-015-1805-5
70 sgo:license sg:explorer/license/
71 sgo:sdDataset articles
72 rdf:type schema:ScholarlyArticle
73 N009768ffa0bd4a18a164e247c52817d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Cell Enlargement
75 rdf:type schema:DefinedTerm
76 N0a461a64402f443dbcd45b77cfe56f07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name SOX9 Transcription Factor
78 rdf:type schema:DefinedTerm
79 N140c18f11c294ece82edf6d79942a6a3 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
80 schema:familyName Young
81 schema:givenName Peter
82 rdf:type schema:Person
83 N1ad669dc1c384499ae72df5a1ff3df70 rdf:first sg:person.01015736676.90
84 rdf:rest rdf:nil
85 N1d564fceec0b455ab84dc60e4dc10212 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Gene Expression Profiling
87 rdf:type schema:DefinedTerm
88 N1eb8cf693c2e4343966dde62ed020265 rdf:first sg:person.01336263356.06
89 rdf:rest N1ad669dc1c384499ae72df5a1ff3df70
90 N2aacbe44025a4fbd9aad175fcffa0352 schema:name pubmed_id
91 schema:value 25813774
92 rdf:type schema:PropertyValue
93 N2e95e193d39c4ffba5cdc61d09ec21ef schema:name dimensions_id
94 schema:value pub.1034813768
95 rdf:type schema:PropertyValue
96 N549a724873b4472cae3616b86f9d9046 schema:name doi
97 schema:value 10.1007/s10529-015-1805-5
98 rdf:type schema:PropertyValue
99 N5de096ae7a5e407aaa2d82d2597600e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Chondrocytes
101 rdf:type schema:DefinedTerm
102 N5e92305f97c045459e8849f34fd39fe1 schema:name Springer Nature - SN SciGraph project
103 rdf:type schema:Organization
104 N72ac4911c4864e07bbd70a1a9e814fa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Cell Dedifferentiation
106 rdf:type schema:DefinedTerm
107 N793d7165a3484ac0bb65cd9b3069363f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Transcription Factors
109 rdf:type schema:DefinedTerm
110 N8a87d1f1c664432c9293e0fb33eeb316 schema:name nlm_unique_id
111 schema:value 8008051
112 rdf:type schema:PropertyValue
113 Nb21e0b06891f44668c07c7130fa5d3de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Lentivirus
115 rdf:type schema:DefinedTerm
116 Ncb00a684108c4107b77b18950a6cbd60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Male
118 rdf:type schema:DefinedTerm
119 Nd247830ffd14454a9de6fee78bf18faf schema:volumeNumber 37
120 rdf:type schema:PublicationVolume
121 Ne2f4bb891c5249deb888160527d151ba schema:issueNumber 7
122 rdf:type schema:PublicationIssue
123 Ne5718169281f48f78d7d517454053fb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Animals
125 rdf:type schema:DefinedTerm
126 Ned3175a04c694a36a0959fb761db2e78 rdf:first sg:person.01200356350.18
127 rdf:rest Nf3278fcec3984b97b9bba5dfc83105b2
128 Need00243f9cb4d65ae6d6efd2f680d2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Dogs
130 rdf:type schema:DefinedTerm
131 Nf3278fcec3984b97b9bba5dfc83105b2 rdf:first N140c18f11c294ece82edf6d79942a6a3
132 rdf:rest N1eb8cf693c2e4343966dde62ed020265
133 Nf52c37900382469e836bd71a6ab4328c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cells, Cultured
135 rdf:type schema:DefinedTerm
136 Nfdcaf7a9582442d7b61b9de8fa9cd916 schema:name readcube_id
137 schema:value a5d6e401449d01c319d2d4c8e7726529fc82b1e6d26c98e8ddbfdf92971310f9
138 rdf:type schema:PropertyValue
139 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
140 schema:name Biological Sciences
141 rdf:type schema:DefinedTerm
142 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biochemistry and Cell Biology
144 rdf:type schema:DefinedTerm
145 sg:journal.1289037 schema:issn 0951-208X
146 1573-6784
147 schema:name Biotechnology Letters
148 rdf:type schema:Periodical
149 sg:person.01015736676.90 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
150 schema:familyName Strappe
151 schema:givenName Padraig
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015736676.90
153 rdf:type schema:Person
154 sg:person.01200356350.18 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
155 schema:familyName Gurusinghe
156 schema:givenName Saliya
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200356350.18
158 rdf:type schema:Person
159 sg:person.01336263356.06 schema:affiliation https://www.grid.ac/institutes/grid.1037.5
160 schema:familyName Michelsen
161 schema:givenName Jacob
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336263356.06
163 rdf:type schema:Person
164 sg:pub.10.1007/s10529-010-0514-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008222869
165 https://doi.org/10.1007/s10529-010-0514-3
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/s11427-011-4179-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007516003
168 https://doi.org/10.1007/s11427-011-4179-5
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/s12015-011-9239-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020262768
171 https://doi.org/10.1007/s12015-011-9239-5
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s12015-014-9538-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022550684
174 https://doi.org/10.1007/s12015-014-9538-8
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s12265-010-9233-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030593249
177 https://doi.org/10.1007/s12265-010-9233-3
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/cdd.2011.137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003363495
180 https://doi.org/10.1038/cdd.2011.137
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1002/0471142301.nsa01cs01 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032097665
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1002/art.20611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016782209
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1002/jcb.24388 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005256675
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1002/jor.20523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005195862
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/0014-5793(94)80292-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012831262
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.bbrc.2011.06.194 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029044921
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.cell.2010.03.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022874771
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.cell.2012.08.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036662790
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.joca.2004.10.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000430028
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/j.joca.2013.01.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042240293
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/s0006-291x(02)03026-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013665217
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1042/0264-6021:3600461 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056715880
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1056/nejm199410063311401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046886095
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1074/jbc.m109.076992 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022161308
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1074/jbc.m110.150540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034657805
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1074/jbc.m413913200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053042379
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1083/jcb.201308102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045700279
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1089/107632704323061933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059206402
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1089/hum.2004.15.976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272421
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1089/scd.2008.0335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059309915
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1155/2012/429160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038504947
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1172/jci44605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026708846
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1371/journal.pone.0022008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025307841
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1371/journal.pone.0077365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001679209
233 rdf:type schema:CreativeWork
234 https://doi.org/10.2527/jas.2010-3666 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070887719
235 rdf:type schema:CreativeWork
236 https://www.grid.ac/institutes/grid.1037.5 schema:alternateName Charles Sturt University
237 schema:name Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW, Australia
238 School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia
239 School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia
240 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...