Preparation of submicrometer monodispersed magnetic silica particles using a novel water in oil microemulsion: properties and application for enzyme immobilization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-06-26

AUTHORS

Maria Victoria Tuttolomondo, Maria Emilia Villanueva, Gisela Solange Alvarez, Martín Federico Desimone, Luis Eduardo Díaz

ABSTRACT

The synthesis of monodispersed magnetic silica nanoparticles (MSN) is described using a water-in-oil reverse microemulsion system that does not require the use of co-surfactants. Sodium silicate, Tween 20 as a neutral surfactant and 1-butanol as the organic phase were used. There are several advantages of the proposed method including a saturation magnetization value of 10 emu/g for the particles obtained, uniformity of size and that they are easily functionalized to bind urease covalently. Moreover, the intra-day, inter-day and long-term stability results confirm that the procedure was successful and the enzyme-linked MSNs were stable over repeated uses and storage retaining more than 75 % activity after 4 months. More... »

PAGES

1571-1577

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-013-1259-6

DOI

http://dx.doi.org/10.1007/s10529-013-1259-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040690004

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23801114


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