Proteomic analysis of the impact of static culturing on the expansion of rat bone marrow mesenchymal stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-05-08

AUTHORS

Stefan Peter, Caroline Evans, Saw Y. Ow, Andy M. Scutt, Phillip C. Wright, Catherine A. Biggs

ABSTRACT

The clinical potential of mesenchymal stem cells (MSC) in tissue engineering and regenerative medicine is due to their self-renewal, proliferation and multi-lineage differentiation potential. Clinical use requires large cell numbers; which can, theoretically, be generated by ex vivo expansion of plastic adherent, MSC subpopulation, of bone marrow cells (BMC). Effects of serial culture on MSC phenotype were investigated using non-gel based quantitative proteomic methodology for static monolayer cultures of rat BMC. In total, 382 proteins were relatively quantified (≥2 peptides). Nine proteins were up-regulated and seven down-regulated at passage 4 relative to passage 2 (p ≤ 0.05). We propose that serial culture impacts on MSC expansion (observed decline in colony forming potential and colony size) is through a combination of osteogenic differentiation and ageing/senescence and propose six novel protein biomarkers as candidates for quality control purposes in bioprocessing. More... »

PAGES

1589-1596

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-012-0935-2

DOI

http://dx.doi.org/10.1007/s10529-012-0935-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051469139

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22566207


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