Improvement of d-lactate productivity in recombinant Escherichia coli by coupling production with growth View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-06

AUTHORS

Li Zhou, Kang-Ming Tian, Dan-Dan Niu, Wei Shen, Gui-Yang Shi, Suren Singh, Zheng-Xiang Wang

ABSTRACT

Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm and 42°C, achieved a twofold higher productivity of lactic acid compared to aerobic and O(2)-limited two-phase fermentation. The cells in the bioreactor yielded an overall volumetric productivity of 5.5 g/l h and a yield of 86 g lactic acid/100 g glucose which were 66% higher and the same level compared to that of the aerobic and O(2)-limited two-phase fermentation, respectively, using scaled-up conditions optimized from shake-flask experiments. These results have revealed an approach for improving production of fermentative products in E. coli. More... »

PAGES

1123-1130

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-012-0883-x

DOI

http://dx.doi.org/10.1007/s10529-012-0883-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014474237

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22367280


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