Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-06

AUTHORS

Byoung Sam Ko, Dong-Min Kim, Byoung Hoon Yoon, Suk Bai, Hyeon Yong Lee, Jung Hoe Kim, Il-Chul Kim

ABSTRACT

To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production. More... »

PAGES

1209-1213

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-011-0558-z

DOI

http://dx.doi.org/10.1007/s10529-011-0558-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052675133

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21331586


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