Rachiplusia nu larva as a biofactory to achieve high level expression of horseradish peroxidase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-05

AUTHORS

Lucía Virginia Romero, Alexandra Marisa Targovnik, Federico Javier Wolman, Osvaldo Cascone, María Victoria Miranda

ABSTRACT

A process based on orally-infected Rachiplusia nu larvae as biological factories for expression and one-step purification of horseradish peroxidase isozyme C (HRP-C) is described. The process allows obtaining high levels of pure HRP-C by membrane chromatography purification. The introduction of the partial polyhedrin homology sequence element in the target gene increased HRP-C expression level by 2.8-fold whereas it increased 1.8-fold when the larvae were reared at 27 °C instead of at 24 °C, summing up a 4.6-fold overall increase in the expression level. Additionally, HRP-C purification by membrane chromatography at a high flow rate greatly increase D the productivity without affecting the resolution. The V(max) and K(m) values of the recombinant HRP-C were similar to those of the HRP from Armoracia rusticana roots. More... »

PAGES

947-956

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-011-0540-9

DOI

http://dx.doi.org/10.1007/s10529-011-0540-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042262852

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21287234


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