l-Arabinose pathway engineering for arabitol-free xylitol production in Candida tropicalis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-04

AUTHORS

Byoung Hoon Yoon, Woo Young Jeon, Woo Yong Shim, Jung Hoe Kim

ABSTRACT

Xylose reductase (XR) is a key enzyme in biological xylitol production, and most XRs have broad substrate specificities. During xylitol production from biomass hydrolysate, non-specific XRs can reduce L-arabinose, which is the second-most abundant hemicellulosic sugar, to the undesirable byproduct arabitol, which interferes with xylitol crystallization in downstream processing. To minimize the flux from L-arabinose to arabitol, the L-arabinose-preferring, endogenous XR was replaced by a D-xylose-preferring heterologous XR in Candida tropicalis. Then, Bacillus licheniformis araA and Escherichia coli araB and araD were codon-optimized and expressed functionally in C. tropicalis for the efficient assimilation of L-arabinose. During xylitol fermentation, the control strains BSXDH-3 and KNV converted 9.9 g L-arabinose l(-1) into 9.5 and 8.3 g arabitol l(-1), respectively, whereas the recombinant strain JY consumed 10.5 g L-arabinose l(-1) for cell growth without forming arabitol. Moreover, JY produced xylitol with 42 and 16% higher productivity than BSXDH-3 and KNV, respectively. More... »

PAGES

747-753

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-010-0487-2

DOI

http://dx.doi.org/10.1007/s10529-010-0487-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049305024

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21127946


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arabinose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Candida tropicalis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sugar Alcohols", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xylitol", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, 305-701, Daejeon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoon", 
        "givenName": "Byoung Hoon", 
        "id": "sg:person.0673641317.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673641317.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, 305-701, Daejeon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jeon", 
        "givenName": "Woo Young", 
        "id": "sg:person.01123077116.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123077116.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, 305-701, Daejeon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shim", 
        "givenName": "Woo Yong", 
        "id": "sg:person.01351132217.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351132217.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, 305-701, Daejeon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Jung Hoe", 
        "id": "sg:person.01300137557.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300137557.41"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00253-008-1652-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001485881", 
          "https://doi.org/10.1007/s00253-008-1652-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-008-1652-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001485881", 
          "https://doi.org/10.1007/s00253-008-1652-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.69.7.4144-4150.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020468925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02699-05", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034984139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(86)90067-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037343030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(86)90067-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037343030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cbic.200700765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042228005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abbi.2001.2613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046828006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02395-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049347601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080653778", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-04", 
    "datePublishedReg": "2011-04-01", 
    "description": "Xylose reductase (XR) is a key enzyme in biological xylitol production, and most XRs have broad substrate specificities. During xylitol production from biomass hydrolysate, non-specific XRs can reduce L-arabinose, which is the second-most abundant hemicellulosic sugar, to the undesirable byproduct arabitol, which interferes with xylitol crystallization in downstream processing. To minimize the flux from L-arabinose to arabitol, the L-arabinose-preferring, endogenous XR was replaced by a D-xylose-preferring heterologous XR in Candida tropicalis. Then, Bacillus licheniformis araA and Escherichia coli araB and araD were codon-optimized and expressed functionally in C. tropicalis for the efficient assimilation of L-arabinose. During xylitol fermentation, the control strains BSXDH-3 and KNV converted 9.9 g L-arabinose l(-1) into 9.5 and 8.3 g arabitol l(-1), respectively, whereas the recombinant strain JY consumed 10.5 g L-arabinose l(-1) for cell growth without forming arabitol. Moreover, JY produced xylitol with 42 and 16% higher productivity than BSXDH-3 and KNV, respectively.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10529-010-0487-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "33"
      }
    ], 
    "name": "l-Arabinose pathway engineering for arabitol-free xylitol production in Candida tropicalis", 
    "pagination": "747-753", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "eae1628b57ef63a691fe0c00906c16d73283eb7cc8399f644f22f1e10b212de9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21127946"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8008051"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10529-010-0487-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049305024"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10529-010-0487-2", 
      "https://app.dimensions.ai/details/publication/pub.1049305024"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:33", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000516.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs10529-010-0487-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10529-010-0487-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10529-010-0487-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10529-010-0487-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10529-010-0487-2'


 

This table displays all metadata directly associated to this object as RDF triples.

142 TRIPLES      21 PREDICATES      44 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10529-010-0487-2 schema:about N15fc65913b9a4e5e81c5763afd0073ef
2 N2f043611cb3c48bea0476ef8998774b7
3 N46ef75044d9d46f69b6bae02cff07aae
4 N7348e82b9d994c689064fe98edbd2615
5 Na83981dded7e430d917bc5e937bf7cf7
6 Na944b344a4e640bb8227f2c046308bc2
7 Nab1204fbbb78464ebfef55703c40b88e
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author Ne58a0980b4aa42a69836f12ad21d2d50
11 schema:citation sg:pub.10.1007/s00253-008-1652-6
12 https://app.dimensions.ai/details/publication/pub.1080653778
13 https://doi.org/10.1002/cbic.200700765
14 https://doi.org/10.1006/abbi.2001.2613
15 https://doi.org/10.1016/0378-1119(86)90067-3
16 https://doi.org/10.1128/aem.02395-07
17 https://doi.org/10.1128/aem.02699-05
18 https://doi.org/10.1128/aem.69.7.4144-4150.2003
19 schema:datePublished 2011-04
20 schema:datePublishedReg 2011-04-01
21 schema:description Xylose reductase (XR) is a key enzyme in biological xylitol production, and most XRs have broad substrate specificities. During xylitol production from biomass hydrolysate, non-specific XRs can reduce L-arabinose, which is the second-most abundant hemicellulosic sugar, to the undesirable byproduct arabitol, which interferes with xylitol crystallization in downstream processing. To minimize the flux from L-arabinose to arabitol, the L-arabinose-preferring, endogenous XR was replaced by a D-xylose-preferring heterologous XR in Candida tropicalis. Then, Bacillus licheniformis araA and Escherichia coli araB and araD were codon-optimized and expressed functionally in C. tropicalis for the efficient assimilation of L-arabinose. During xylitol fermentation, the control strains BSXDH-3 and KNV converted 9.9 g L-arabinose l(-1) into 9.5 and 8.3 g arabitol l(-1), respectively, whereas the recombinant strain JY consumed 10.5 g L-arabinose l(-1) for cell growth without forming arabitol. Moreover, JY produced xylitol with 42 and 16% higher productivity than BSXDH-3 and KNV, respectively.
22 schema:genre research_article
23 schema:inLanguage en
24 schema:isAccessibleForFree false
25 schema:isPartOf N1846848c73bd43478440ccae7c2d3811
26 N78e7350189d9416385a3b717d34034c5
27 sg:journal.1289037
28 schema:name l-Arabinose pathway engineering for arabitol-free xylitol production in Candida tropicalis
29 schema:pagination 747-753
30 schema:productId N3ed4f50804f14b3583aa784ac4d9639a
31 N5f91d86b13a24cc48aa4e00b22879ed9
32 N7b20ef9448d346f3ae1319ae04bd7e6d
33 N7d36e0ba9ab64ae592a88ba65e5936e2
34 Nc52e6287afcf4817861fa14dcbbb42ba
35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049305024
36 https://doi.org/10.1007/s10529-010-0487-2
37 schema:sdDatePublished 2019-04-10T17:33
38 schema:sdLicense https://scigraph.springernature.com/explorer/license/
39 schema:sdPublisher Nd8c340530b74485d9c4add1c46c0dff2
40 schema:url http://link.springer.com/10.1007%2Fs10529-010-0487-2
41 sgo:license sg:explorer/license/
42 sgo:sdDataset articles
43 rdf:type schema:ScholarlyArticle
44 N0eb99f603a7e4310b9a4d5522cd29e91 rdf:first sg:person.01123077116.78
45 rdf:rest N1324852a29aa4a60b4882cfe554f0d7c
46 N1324852a29aa4a60b4882cfe554f0d7c rdf:first sg:person.01351132217.40
47 rdf:rest N3efbea9b995f466e9d6adaff1eb15d2c
48 N15fc65913b9a4e5e81c5763afd0073ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
49 schema:name Signal Transduction
50 rdf:type schema:DefinedTerm
51 N1846848c73bd43478440ccae7c2d3811 schema:volumeNumber 33
52 rdf:type schema:PublicationVolume
53 N2f043611cb3c48bea0476ef8998774b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Candida tropicalis
55 rdf:type schema:DefinedTerm
56 N3ed4f50804f14b3583aa784ac4d9639a schema:name readcube_id
57 schema:value eae1628b57ef63a691fe0c00906c16d73283eb7cc8399f644f22f1e10b212de9
58 rdf:type schema:PropertyValue
59 N3efbea9b995f466e9d6adaff1eb15d2c rdf:first sg:person.01300137557.41
60 rdf:rest rdf:nil
61 N46ef75044d9d46f69b6bae02cff07aae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Xylitol
63 rdf:type schema:DefinedTerm
64 N5f91d86b13a24cc48aa4e00b22879ed9 schema:name dimensions_id
65 schema:value pub.1049305024
66 rdf:type schema:PropertyValue
67 N7348e82b9d994c689064fe98edbd2615 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Reverse Transcriptase Polymerase Chain Reaction
69 rdf:type schema:DefinedTerm
70 N78e7350189d9416385a3b717d34034c5 schema:issueNumber 4
71 rdf:type schema:PublicationIssue
72 N7b20ef9448d346f3ae1319ae04bd7e6d schema:name doi
73 schema:value 10.1007/s10529-010-0487-2
74 rdf:type schema:PropertyValue
75 N7d36e0ba9ab64ae592a88ba65e5936e2 schema:name nlm_unique_id
76 schema:value 8008051
77 rdf:type schema:PropertyValue
78 Na83981dded7e430d917bc5e937bf7cf7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Arabinose
80 rdf:type schema:DefinedTerm
81 Na944b344a4e640bb8227f2c046308bc2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Protein Engineering
83 rdf:type schema:DefinedTerm
84 Nab1204fbbb78464ebfef55703c40b88e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Sugar Alcohols
86 rdf:type schema:DefinedTerm
87 Nc52e6287afcf4817861fa14dcbbb42ba schema:name pubmed_id
88 schema:value 21127946
89 rdf:type schema:PropertyValue
90 Nd8c340530b74485d9c4add1c46c0dff2 schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 Ne58a0980b4aa42a69836f12ad21d2d50 rdf:first sg:person.0673641317.14
93 rdf:rest N0eb99f603a7e4310b9a4d5522cd29e91
94 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
95 schema:name Biological Sciences
96 rdf:type schema:DefinedTerm
97 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
98 schema:name Biochemistry and Cell Biology
99 rdf:type schema:DefinedTerm
100 sg:journal.1289037 schema:issn 0951-208X
101 1573-6784
102 schema:name Biotechnology Letters
103 rdf:type schema:Periodical
104 sg:person.01123077116.78 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
105 schema:familyName Jeon
106 schema:givenName Woo Young
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123077116.78
108 rdf:type schema:Person
109 sg:person.01300137557.41 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
110 schema:familyName Kim
111 schema:givenName Jung Hoe
112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300137557.41
113 rdf:type schema:Person
114 sg:person.01351132217.40 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
115 schema:familyName Shim
116 schema:givenName Woo Yong
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351132217.40
118 rdf:type schema:Person
119 sg:person.0673641317.14 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
120 schema:familyName Yoon
121 schema:givenName Byoung Hoon
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673641317.14
123 rdf:type schema:Person
124 sg:pub.10.1007/s00253-008-1652-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001485881
125 https://doi.org/10.1007/s00253-008-1652-6
126 rdf:type schema:CreativeWork
127 https://app.dimensions.ai/details/publication/pub.1080653778 schema:CreativeWork
128 https://doi.org/10.1002/cbic.200700765 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042228005
129 rdf:type schema:CreativeWork
130 https://doi.org/10.1006/abbi.2001.2613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046828006
131 rdf:type schema:CreativeWork
132 https://doi.org/10.1016/0378-1119(86)90067-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037343030
133 rdf:type schema:CreativeWork
134 https://doi.org/10.1128/aem.02395-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049347601
135 rdf:type schema:CreativeWork
136 https://doi.org/10.1128/aem.02699-05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034984139
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1128/aem.69.7.4144-4150.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020468925
139 rdf:type schema:CreativeWork
140 https://www.grid.ac/institutes/grid.37172.30 schema:alternateName Korea Advanced Institute of Science and Technology
141 schema:name Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, 305-701, Daejeon, Korea
142 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...