Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-04-10

AUTHORS

Wei Ji, Yanming Zhu, Yong Li, Liang Yang, Xiaowen Zhao, Hua Cai, Xi Bai

ABSTRACT

Glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. We constructed a cDNA library from G. soja seedlings treated with NaCl and isolated a glutathione S-transferase gene (GsGST: GQ265911) from the library. The cDNA encoding GsGST contains an open reading frame of 660 bp and the predicted protein belongs to the tau class of GST family proteins. Tobacco plants over-expressing the GsGST gene showed sixfold higher GST activity than wild-type plants. Transgenic tobacco plants exhibited enhanced dehydration tolerance. T2 transgenic tobacco plants showed higher tolerance at the seedling stage than wild-type plants to salt and mannitol as demonstrated by longer root length and less growth retardation. More... »

PAGES

1173-1179

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-010-0269-x

DOI

http://dx.doi.org/10.1007/s10529-010-0269-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046533594

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20383560


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