Encapsulation of recombinant E. coli expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-05

AUTHORS

Martin Hucík, Marek Bučko, Peter Gemeiner, Vladimír Štefuca, Alica Vikartovská, Marko D. Mihovilovič, Florian Rudroff, Naseem Iqbal, Dušan Chorvát, Igor Lacík

ABSTRACT

Recombinant Escherichia coli cells, over-expressing cyclopentanone monooxygenase activity, were immobilized in polyelectrolyte complex capsules, made of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl(2) and NaCl. More than 90% of the cell viability was preserved during the encapsulation process. Moreover, the initial enzyme activity was fully maintained within encapsulated cells while it halved in free cells. Both encapsulated and free cells reached the end point of the Baeyer-Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one to 4,9-dioxabicyclo[4.2.1]non-7-en-3-one at the same time (48 h). Similarly, the enantiomeric excess above 94% was identical for encapsulated and free cells. More... »

PAGES

675-680

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-010-0203-2

DOI

http://dx.doi.org/10.1007/s10529-010-0203-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022541780

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20111981


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