Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-11-05

AUTHORS

Jong Hyun Yoon, Eun Youn Roh, Sue Shin, Nam Hee Jung, Eun Young Song, Dong Soon Lee, Kyou Sup Han, Joung Sung Kim, Byoung Jae Kim, Hye Won Jeon, Kang Sup Yoon

ABSTRACT

The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 × 105 cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation. More... »

PAGES

329-335

Journal

TITLE

Biotechnology Letters

ISSUE

3

VOLUME

31

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-008-9872-5

DOI

http://dx.doi.org/10.1007/s10529-008-9872-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042418634

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18985278


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