Cardiac cells implanted into a cylindrical, vascularized chamber in vivo: pressure generation and morphology View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-02

AUTHORS

Ravi K. Birla, Vikas Dhawan, Douglas E. Dow, Yen-Chih Huang, David L. Brown

ABSTRACT

We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were isolated from hearts of 2-3 day old rats, suspended in fibrin gel and inserted into the lumen of silicone tubing. The silicone tubing was then implanted around the femoral vessels in the groin region of recipient animals. After 3 weeks, the constructs were harvested, placed in an in vitro bath and cannulated via the incorporated femoral artery with a pressure transducer for evaluation of intra-luminal pressure dynamics. Histological evaluation showed the presence of a concentric ring of cardiac cells surrounding the femoral vessels. There was also a significant amount of collagen present around cardiac cells. In addition, we observed a significant amount of neovascularization of the explanted constructs. Electron microscopy showed the presence of longitudinally aligned fibers with a large number of gap junctions. Upon electrical stimulation of a single pulse (7 V, 1.2 ms), the constructs generated an intra-luminal pressure of 1.19 +/- 0.45 mmHg (n = 6). In addition, we were able to electrically pace the constructs at frequencies of 0.5-5 Hz. A Starling behavior of the inverse relation between baseline pressure and twitch pressure was observed. Cardiac cells implanted for 3 weeks into the cylindrical vascularized bed formed a tissue construct that demonstrated many of the contractile properties and morphology expected of functioning cardiac tissues. More... »

PAGES

191-201

References to SciGraph publications

  • 2001-01. Heart Failure Therapy at the Turn of the Century in HEART FAILURE REVIEWS
  • 2002-05. 3D engineered heart tissue for replacement therapy in BASIC RESEARCH IN CARDIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10529-008-9859-2

    DOI

    http://dx.doi.org/10.1007/s10529-008-9859-2

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18854950


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