Engineering a native homoethanol pathway in Escherichia coli B for ethanol production View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-02

AUTHORS

S. Zhou, A. G. Iverson, W. S. Grayburn

ABSTRACT

A native homoethanol pathway (pyruvate-to-acetyl-CoA-to-acetaldehyde-to-ethanol) was engineered in Escherichia coli B. The competing fermentation pathways were eliminated by chromosomal deletions of the genes encoding for fumarate reductase (frdABCD), lactate dehydrogenase (ldhA), acetate kinase (ackA), and pyruvate formate lyase (pflB). For redox balance and anaerobic cell growth, the pyruvate dehydrogenase complex (aceEF-lpd, a typical aerobically-expressed operon) was highly expressed anaerobically using a native anaerobic inducible promoter. The resulting strain SZ420 (DeltafrdBC DeltaldhA DeltaackA DeltafocA-pflB DeltapdhR::pflBp6-pflBrbs-aceEF-lpd) contains no foreign genes and/or promoters and efficiently ferments glucose and xylose into ethanol with a yield of 90% under anaerobic conditions. More... »

PAGES

335-342

Journal

TITLE

Biotechnology Letters

ISSUE

2

VOLUME

30

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10529-007-9544-x

    DOI

    http://dx.doi.org/10.1007/s10529-007-9544-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020849928

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17957344


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