pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-11

AUTHORS

Claudia E. Vickers, Peer M. Schenk, Dongxue Li, Philip M. Mullineaux, Peter M. Gresshoff

ABSTRACT

A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls. More... »

PAGES

1793-1796

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-007-9467-6

DOI

http://dx.doi.org/10.1007/s10529-007-9467-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024941577

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17687623


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