Rational selection of an antibody probe to detect the heterogeneous collection of CHO-derived rhGM-CSF glycoforms View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-12

AUTHORS

Marcos Oggero, Guillermina Forno, Ricardo Kratje, Marina Etcheverrigaray

ABSTRACT

Six anti-E. coli rhGM-CSF monoclonal antibodies were generated using hybridoma technology. One of these showed identical affinity for the CHO and E. coli-derived cytokine (dissociation constants of 0.14 +/- 0.01 nM and 0.13 +/- 0.01 nM, respectively), mapping an epitope that is not hindered by carbohydrates. The antibody was used to develop a simple, specific and sensitive competitive ELISA to quantify the entire set of rhGM-CSF glycoforms (detection limit of 780 pg/ml) and it was successful as an affinity ligand to purify them. Therefore, this particular antibody is a useful, reliable and reagent for most immunochemical purposes with the aim of detecting, quantifying and purifying the highly heterogeneous collection of the CHO-derived rhGM-CSF isoforms. More... »

PAGES

2049-2056

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-006-9187-3

DOI

http://dx.doi.org/10.1007/s10529-006-9187-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030559975

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17004006


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