Over-production of β-carotene from metabolically engineered Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-06

AUTHORS

Sung-Woo Kim, Jae-Bum Kim, W.-H. Jung, Jung-Hoe Kim, Joon-Ki Jung

ABSTRACT

To produce recombinant beta-carotene in vitro, synthetic operons encoding genes governing its biosynthesis were engineered into Escherichia coli. Constructs harboring these operons were introduced into either a high-copy or low-copy cloning vector. beta-Carotene production from these recombinant E. coli cells was either constitutive or inducible depending upon plasmid copy number. The most efficient beta-carotene production was with the low-copy based vector. The process was increased incrementally from a 5 l to a 50 l fermentor and finally into a 300 l fermentor. The maximal beta-carotene yields achieved using the 50 l and 300 l fermentor were 390 mg l(-1) and 240 mg l(-1), respectively, with overall productivities of 7.8 mg l(-1) h(-1) and 4.8 mg l(-1) h(-1). More... »

PAGES

897-904

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-006-9023-9

DOI

http://dx.doi.org/10.1007/s10529-006-9023-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000247706

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16786275


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