Rapid Affinity Purification Processes for Cyclodextrin Glycosyltransferase from Bacilluscirculans View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-08

AUTHORS

A. Rosso, S. Ferrarotti, M.V. Miranda, N. Krymkiewicz, B.C. Nudel, O. Cascone

ABSTRACT

Two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. The first method, optimised by a factorial design, gave an 80% CGTase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mM alpha-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72% CGTase recovery in a two-step procedure; CGTase was obtained in the bottom phase with a purification factor of 37. More... »

PAGES

1171-1175

References to SciGraph publications

  • 1999-12. Strategies for improving fermentation medium performance: a review in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10529-005-8654-6

    DOI

    http://dx.doi.org/10.1007/s10529-005-8654-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1008058371

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16158259


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