Cloning and Production of a Novel Bacteriocin, Lactococcin K, from Lactococcus lactis Subsp. lactis MY23 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-03

AUTHORS

Yun Seog Kim, Min Ju Kim, Pil Kim, Jung Hoe Kim

ABSTRACT

A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml(-1) in 12 h. More... »

PAGES

357-362

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-005-5935-z

DOI

http://dx.doi.org/10.1007/s10529-005-5935-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030582907

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16614924


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