Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-12

AUTHORS

S. Zhou, L.P. Yomano, K.T. Shanmugam, L.O. Ingram

ABSTRACT

Derivatives of ethanologenic Escherichia coli K011 were constructed for D: (-)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based selection for mutants with improved performance. Resulting strains, SZ132 and SZ186, contain native genes for sucrose utilization. No foreign genes are present in SZ186. Strain SZ132 also contains a chromosomally integrated endoglucanase gene (Erwinia chrysanthemi celY). Strain SZ132 produced over 1 mol lactate per liter of complex medium containing 10% (w/v) sugar (fermentation times of 48 h for glucose, 120 h for sucrose). Both strains produced 667-700 mmol lactate per liter of mineral salts medium. Yields for metabolized sugar ranged from 88% to 95% in both media. More... »

PAGES

1891-1896

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10529-005-3899-7

DOI

http://dx.doi.org/10.1007/s10529-005-3899-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032328145

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16328986


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