Cloning and characterization of the bifunctional alcohol/acetaldehyde dehydrogenase gene (adhE) in Leuconostoc mesenteroides isolated from kimchi View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-04

AUTHORS

Ok Kyung Koo, Do-Won Jeong, Jung Min Lee, Min Jung Kim, Jong-Hoon Lee, Hae Choon Chang, Jeong Hwan Kim, & Hyong Joo Lee

ABSTRACT

A bifunctional alcohol/acetaldehyde dehydrogenase (AdhE) gene (adhE) was cloned from Leuconostoc mesenteroides C7 (LMC7), which is the dominant lactic acid bacterium produced during heterofermentation of kimchi. The nucleotide sequence of the DNA fragment containing putative adhE, which is 2685 bp long and encodes an 886 amino acid polypeptide, exhibits 99% homology with Leu. mesenteroides sp. cremoris. The deduced AdhE comprises two conserved domains: alcohol dehydrogenase (Adh) and acetaldehyde dehydrogenase (Aldh). Moreover, two NAD-binding sites were observed, based on the presence of the GXGXXG motif. A pADHE containing the adhE gene expressed AdhE at the translational level in Escherichia coli BL21, which was at a higher level than in E. coli DH5alpha and E. coli JM109. The AdhE of LMC7 showed Adh and Aldh activities that, when expressed in E. coli. BL21, were 7.5 and 5.7 U mg(-1) , respectively. More... »

PAGES

505-510

Journal

TITLE

Biotechnology Letters

ISSUE

7

VOLUME

27

Related Patents

  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms And Methods For The Co-Production Of Isopropanol And 1,4-Butanediol
  • Microorganisms For Producing Butadiene And Methods Related Thereto
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For Production Of 4-Hydroxybutyrate, 1,4-Butanediol And Related Compounds
  • Microorganisms And Methods For Enhancing The Availability Of Reducing Equivalents In The Presence Of Methanol, And For Producing 1,2-Propanediol, N-Propanol, 1,3-Propanediol, Or Glycerol Related Thereto
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Methods And Organisms For Converting Synthesis Gas Or Other Gaseous Carbon Sources And Methanol To 1,3-Butanediol
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Methods And Organisms For Converting Synthesis Gas Or Other Gaseous Carbon Sources And Methanol To 1,3-Butanediol
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms And Methods For The Biosynthesis Of Propylene
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Process Of Separating Components Of A Fermentation Broth
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Microorganisms And Methods For The Production Of Caprolactone
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Methods For The Synthesis Of Olefins And Derivatives
  • Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms For The Production Of 2-Hydroxyisobutyric Acid
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Fumarate, Malate, And Acrylate
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms For Producing Butadiene And Methods Related Thereto
  • Microorganisms And Methods For The Production Of Caprolactone
  • Organisms For The Production Of 1,3-Butanediol
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Organisms For The Production Of 1,3-Butanediol
  • Microbial Organisms Comprising Exogenous Nucleic Acids Encoding Reductive Tca Pathway Enzymes
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Methods And Organisms For The Growth-Coupled Production Of 1,4-Butanediol
  • Microorganisms For The Production Of 1,4-Butanediol
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms And Methods For Enhancing The Availability Of Reducing Equivalents In The Presence Of Methanol, And For Producing 1,4-Butanediol Related Thereto
  • Microorganisms And Methods For The Production Of Caprolactone
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Primary Alcohol Producing Organisms
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods For Increasing Product Yields
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Microorganisms For The Production Of 1,4-Butanediol
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods And Organisms For The Growth-Coupled Production Of 1,4-Butanediol
  • Organisms For The Production Of Isopropanol, N-Butanol, And Isobutanol
  • Primary Alcohol Producing Organisms
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10529-005-2541-z

    DOI

    http://dx.doi.org/10.1007/s10529-005-2541-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1042475206

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15928858


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alcohol Dehydrogenase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Aldehyde Oxidoreductases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Motifs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Binding Sites", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Conserved Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Leuconostoc", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Multienzyme Complexes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Structure, Tertiary", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Homology, Amino Acid", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Seoul National University", 
              "id": "https://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, 151-742, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Koo", 
            "givenName": "Ok Kyung", 
            "id": "sg:person.0750700175.71", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750700175.71"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Seoul National University", 
              "id": "https://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, 151-742, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jeong", 
            "givenName": "Do-Won", 
            "id": "sg:person.01313634206.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313634206.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Seoul National University", 
              "id": "https://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, 151-742, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Jung Min", 
            "id": "sg:person.01164772300.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164772300.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Seoul National University", 
              "id": "https://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, 151-742, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Min Jung", 
            "id": "sg:person.010445015454.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010445015454.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Kyonggi University", 
              "id": "https://www.grid.ac/institutes/grid.411203.5", 
              "name": [
                "Department of Food Science and Biotechnology, Kyonggi University, 443-760, Suwon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Jong-Hoon", 
            "id": "sg:person.01113445705.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113445705.12"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Chosun University", 
              "id": "https://www.grid.ac/institutes/grid.254187.d", 
              "name": [
                "Department of Food and Nutrition, Chosun University, 501-759, Kwangju, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chang", 
            "givenName": "Hae Choon", 
            "id": "sg:person.01347457620.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347457620.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Gyeongsang National University", 
              "id": "https://www.grid.ac/institutes/grid.256681.e", 
              "name": [
                "Division of Applied Life Science, Graduate School, Gyeongsang National University, 660-701, Jinju, Republic\u2423of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Jeong Hwan", 
            "id": "sg:person.0766120715.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766120715.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Seoul National University", 
              "id": "https://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, 151-742, Seoul, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "& Hyong Joo", 
            "id": "sg:person.014152127654.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014152127654.08"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/s0168-1605(96)01233-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000587692"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2000.tb08872.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006485178"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2000.tb08872.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006485178"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.74.12.5463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025360556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m005464200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026772314"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00203-003-0638-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031857146", 
              "https://doi.org/10.1007/s00203-003-0638-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1018386731116", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044041392", 
              "https://doi.org/10.1023/a:1018386731116"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.176.3.871-885.1994", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062723411"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083275629", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2005-04", 
        "datePublishedReg": "2005-04-01", 
        "description": "A bifunctional alcohol/acetaldehyde dehydrogenase (AdhE) gene (adhE) was cloned from Leuconostoc mesenteroides C7 (LMC7), which is the dominant lactic acid bacterium produced during heterofermentation of kimchi. The nucleotide sequence of the DNA fragment containing putative adhE, which is 2685 bp long and encodes an 886 amino acid polypeptide, exhibits 99% homology with Leu. mesenteroides sp. cremoris. The deduced AdhE comprises two conserved domains: alcohol dehydrogenase (Adh) and acetaldehyde dehydrogenase (Aldh). Moreover, two NAD-binding sites were observed, based on the presence of the GXGXXG motif. A pADHE containing the adhE gene expressed AdhE at the translational level in Escherichia coli BL21, which was at a higher level than in E. coli DH5alpha and E. coli JM109. The AdhE of LMC7 showed Adh and Aldh activities that, when expressed in E. coli. BL21, were 7.5 and 5.7 U mg(-1) , respectively.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s10529-005-2541-z", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1289037", 
            "issn": [
              "0951-208X", 
              "1573-6784"
            ], 
            "name": "Biotechnology Letters", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "27"
          }
        ], 
        "name": "Cloning and characterization of the bifunctional alcohol/acetaldehyde dehydrogenase gene (adhE) in Leuconostoc mesenteroides isolated from kimchi", 
        "pagination": "505-510", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "ca5407f4e8b0eb42506345ff8af13c6951a1b51d281c0ec20c13fe3a16357a1a"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "15928858"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8008051"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10529-005-2541-z"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1042475206"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10529-005-2541-z", 
          "https://app.dimensions.ai/details/publication/pub.1042475206"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T14:29", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13087_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs10529-005-2541-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10529-005-2541-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10529-005-2541-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10529-005-2541-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10529-005-2541-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    220 TRIPLES      21 PREDICATES      54 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10529-005-2541-z schema:about N0cf910d5113c41ceb063980661837598
    2 N206ab2612cc949ae90a30916bcebdd38
    3 N29b9e56fa114476ca5c54279a8139552
    4 N409386f4ed7f4905b29a3761eb54f4c0
    5 N56a4d4233155497ba22a0b36a781829e
    6 N7d1170262d3540848d305d08638b0f15
    7 N86cf38b4f9c04cc6ab5b0c0aacec92b4
    8 N8fbbc52c304540faa221713aacd3693d
    9 Naf29fa75e53b46f8b5a72e99bade4fac
    10 Nbac0b2de86bb4ae9ac624b84921e9aa9
    11 Nd169516c7df4463fbe30bb1727451c0d
    12 Ne6944305aa264804adcf7999c6a57199
    13 Ne93816bf7fd945d0b7d882259c4f01bb
    14 Nf8ca5eb12a3b4f5486afe209911c1cbd
    15 Nfa7b727a767e4d2c959b51c1f03c8d25
    16 Nfc503dc2a69d4e5ebdabb7dd3c5f44ae
    17 Nfeec69ddfb714efaa4a7f7d7c7feacec
    18 anzsrc-for:06
    19 anzsrc-for:0604
    20 schema:author Nfc24fdaf5a2840c5b6fdf9250e58719e
    21 schema:citation sg:pub.10.1007/s00203-003-0638-0
    22 sg:pub.10.1023/a:1018386731116
    23 https://app.dimensions.ai/details/publication/pub.1083275629
    24 https://doi.org/10.1016/s0168-1605(96)01233-0
    25 https://doi.org/10.1073/pnas.74.12.5463
    26 https://doi.org/10.1074/jbc.m005464200
    27 https://doi.org/10.1111/j.1574-6968.2000.tb08872.x
    28 https://doi.org/10.1128/jb.176.3.871-885.1994
    29 schema:datePublished 2005-04
    30 schema:datePublishedReg 2005-04-01
    31 schema:description A bifunctional alcohol/acetaldehyde dehydrogenase (AdhE) gene (adhE) was cloned from Leuconostoc mesenteroides C7 (LMC7), which is the dominant lactic acid bacterium produced during heterofermentation of kimchi. The nucleotide sequence of the DNA fragment containing putative adhE, which is 2685 bp long and encodes an 886 amino acid polypeptide, exhibits 99% homology with Leu. mesenteroides sp. cremoris. The deduced AdhE comprises two conserved domains: alcohol dehydrogenase (Adh) and acetaldehyde dehydrogenase (Aldh). Moreover, two NAD-binding sites were observed, based on the presence of the GXGXXG motif. A pADHE containing the adhE gene expressed AdhE at the translational level in Escherichia coli BL21, which was at a higher level than in E. coli DH5alpha and E. coli JM109. The AdhE of LMC7 showed Adh and Aldh activities that, when expressed in E. coli. BL21, were 7.5 and 5.7 U mg(-1) , respectively.
    32 schema:genre research_article
    33 schema:inLanguage en
    34 schema:isAccessibleForFree false
    35 schema:isPartOf N5823ed1dd1874fa2af8ba8861e78f546
    36 N81cf18c8fa9e415ba926d97f901ae754
    37 sg:journal.1289037
    38 schema:name Cloning and characterization of the bifunctional alcohol/acetaldehyde dehydrogenase gene (adhE) in Leuconostoc mesenteroides isolated from kimchi
    39 schema:pagination 505-510
    40 schema:productId N0d2346079d22481b9446e7cba8306807
    41 N235c79e9c98c443a9dea9d13679b029f
    42 N42b281b4eeca4bea9cb3db52461130d0
    43 N50a37c089c104771b00176184e3a755f
    44 Na637478b74b94f9a91944ea7924e5313
    45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042475206
    46 https://doi.org/10.1007/s10529-005-2541-z
    47 schema:sdDatePublished 2019-04-11T14:29
    48 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    49 schema:sdPublisher Nfd728ffaa4fd46b0b1be5d900db52174
    50 schema:url http://link.springer.com/10.1007%2Fs10529-005-2541-z
    51 sgo:license sg:explorer/license/
    52 sgo:sdDataset articles
    53 rdf:type schema:ScholarlyArticle
    54 N0729df92428c46de8c3e9d53e645f013 rdf:first sg:person.01347457620.36
    55 rdf:rest N4789eed7ca5243ac8b5ddf9e9314e8a6
    56 N0cf910d5113c41ceb063980661837598 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    57 schema:name Multienzyme Complexes
    58 rdf:type schema:DefinedTerm
    59 N0d2346079d22481b9446e7cba8306807 schema:name readcube_id
    60 schema:value ca5407f4e8b0eb42506345ff8af13c6951a1b51d281c0ec20c13fe3a16357a1a
    61 rdf:type schema:PropertyValue
    62 N206ab2612cc949ae90a30916bcebdd38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    63 schema:name Molecular Sequence Data
    64 rdf:type schema:DefinedTerm
    65 N235c79e9c98c443a9dea9d13679b029f schema:name dimensions_id
    66 schema:value pub.1042475206
    67 rdf:type schema:PropertyValue
    68 N29b9e56fa114476ca5c54279a8139552 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Escherichia coli
    70 rdf:type schema:DefinedTerm
    71 N3915f8ef64004bd4916d0eafb73f14aa rdf:first sg:person.01164772300.00
    72 rdf:rest Nadb3fa18f2f1494795ffed531e5194a9
    73 N409386f4ed7f4905b29a3761eb54f4c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Conserved Sequence
    75 rdf:type schema:DefinedTerm
    76 N42b281b4eeca4bea9cb3db52461130d0 schema:name doi
    77 schema:value 10.1007/s10529-005-2541-z
    78 rdf:type schema:PropertyValue
    79 N4789eed7ca5243ac8b5ddf9e9314e8a6 rdf:first sg:person.0766120715.35
    80 rdf:rest Ndf65a1f22017467a8500e0e618240bec
    81 N50a37c089c104771b00176184e3a755f schema:name nlm_unique_id
    82 schema:value 8008051
    83 rdf:type schema:PropertyValue
    84 N56a4d4233155497ba22a0b36a781829e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Amino Acid Sequence
    86 rdf:type schema:DefinedTerm
    87 N5823ed1dd1874fa2af8ba8861e78f546 schema:issueNumber 7
    88 rdf:type schema:PublicationIssue
    89 N6587d9a34aba422eb2da6f042e664c4a rdf:first sg:person.01113445705.12
    90 rdf:rest N0729df92428c46de8c3e9d53e645f013
    91 N7d1170262d3540848d305d08638b0f15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Alcohol Dehydrogenase
    93 rdf:type schema:DefinedTerm
    94 N81cf18c8fa9e415ba926d97f901ae754 schema:volumeNumber 27
    95 rdf:type schema:PublicationVolume
    96 N86cf38b4f9c04cc6ab5b0c0aacec92b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Sequence Analysis, DNA
    98 rdf:type schema:DefinedTerm
    99 N8fbbc52c304540faa221713aacd3693d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Amino Acid Motifs
    101 rdf:type schema:DefinedTerm
    102 Na637478b74b94f9a91944ea7924e5313 schema:name pubmed_id
    103 schema:value 15928858
    104 rdf:type schema:PropertyValue
    105 Nadb3fa18f2f1494795ffed531e5194a9 rdf:first sg:person.010445015454.09
    106 rdf:rest N6587d9a34aba422eb2da6f042e664c4a
    107 Naf29fa75e53b46f8b5a72e99bade4fac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Aldehyde Oxidoreductases
    109 rdf:type schema:DefinedTerm
    110 Nbac0b2de86bb4ae9ac624b84921e9aa9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Sequence Homology, Amino Acid
    112 rdf:type schema:DefinedTerm
    113 Nd169516c7df4463fbe30bb1727451c0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Leuconostoc
    115 rdf:type schema:DefinedTerm
    116 Ndf65a1f22017467a8500e0e618240bec rdf:first sg:person.014152127654.08
    117 rdf:rest rdf:nil
    118 Ne6944305aa264804adcf7999c6a57199 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Binding Sites
    120 rdf:type schema:DefinedTerm
    121 Ne93816bf7fd945d0b7d882259c4f01bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Protein Structure, Tertiary
    123 rdf:type schema:DefinedTerm
    124 Neeace5ad578542a5bf6a0c5f8249f915 rdf:first sg:person.01313634206.38
    125 rdf:rest N3915f8ef64004bd4916d0eafb73f14aa
    126 Nf8ca5eb12a3b4f5486afe209911c1cbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Escherichia coli Proteins
    128 rdf:type schema:DefinedTerm
    129 Nfa7b727a767e4d2c959b51c1f03c8d25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Genes, Bacterial
    131 rdf:type schema:DefinedTerm
    132 Nfc24fdaf5a2840c5b6fdf9250e58719e rdf:first sg:person.0750700175.71
    133 rdf:rest Neeace5ad578542a5bf6a0c5f8249f915
    134 Nfc503dc2a69d4e5ebdabb7dd3c5f44ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name DNA, Bacterial
    136 rdf:type schema:DefinedTerm
    137 Nfd728ffaa4fd46b0b1be5d900db52174 schema:name Springer Nature - SN SciGraph project
    138 rdf:type schema:Organization
    139 Nfeec69ddfb714efaa4a7f7d7c7feacec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Cloning, Molecular
    141 rdf:type schema:DefinedTerm
    142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    143 schema:name Biological Sciences
    144 rdf:type schema:DefinedTerm
    145 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    146 schema:name Genetics
    147 rdf:type schema:DefinedTerm
    148 sg:journal.1289037 schema:issn 0951-208X
    149 1573-6784
    150 schema:name Biotechnology Letters
    151 rdf:type schema:Periodical
    152 sg:person.010445015454.09 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
    153 schema:familyName Kim
    154 schema:givenName Min Jung
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010445015454.09
    156 rdf:type schema:Person
    157 sg:person.01113445705.12 schema:affiliation https://www.grid.ac/institutes/grid.411203.5
    158 schema:familyName Lee
    159 schema:givenName Jong-Hoon
    160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113445705.12
    161 rdf:type schema:Person
    162 sg:person.01164772300.00 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
    163 schema:familyName Lee
    164 schema:givenName Jung Min
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164772300.00
    166 rdf:type schema:Person
    167 sg:person.01313634206.38 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
    168 schema:familyName Jeong
    169 schema:givenName Do-Won
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313634206.38
    171 rdf:type schema:Person
    172 sg:person.01347457620.36 schema:affiliation https://www.grid.ac/institutes/grid.254187.d
    173 schema:familyName Chang
    174 schema:givenName Hae Choon
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347457620.36
    176 rdf:type schema:Person
    177 sg:person.014152127654.08 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
    178 schema:familyName Lee
    179 schema:givenName & Hyong Joo
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014152127654.08
    181 rdf:type schema:Person
    182 sg:person.0750700175.71 schema:affiliation https://www.grid.ac/institutes/grid.31501.36
    183 schema:familyName Koo
    184 schema:givenName Ok Kyung
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750700175.71
    186 rdf:type schema:Person
    187 sg:person.0766120715.35 schema:affiliation https://www.grid.ac/institutes/grid.256681.e
    188 schema:familyName Kim
    189 schema:givenName Jeong Hwan
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766120715.35
    191 rdf:type schema:Person
    192 sg:pub.10.1007/s00203-003-0638-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031857146
    193 https://doi.org/10.1007/s00203-003-0638-0
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1023/a:1018386731116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044041392
    196 https://doi.org/10.1023/a:1018386731116
    197 rdf:type schema:CreativeWork
    198 https://app.dimensions.ai/details/publication/pub.1083275629 schema:CreativeWork
    199 https://doi.org/10.1016/s0168-1605(96)01233-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000587692
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1073/pnas.74.12.5463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025360556
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1074/jbc.m005464200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026772314
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1111/j.1574-6968.2000.tb08872.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006485178
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1128/jb.176.3.871-885.1994 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062723411
    208 rdf:type schema:CreativeWork
    209 https://www.grid.ac/institutes/grid.254187.d schema:alternateName Chosun University
    210 schema:name Department of Food and Nutrition, Chosun University, 501-759, Kwangju, Republic of Korea
    211 rdf:type schema:Organization
    212 https://www.grid.ac/institutes/grid.256681.e schema:alternateName Gyeongsang National University
    213 schema:name Division of Applied Life Science, Graduate School, Gyeongsang National University, 660-701, Jinju, Republic␣of Korea
    214 rdf:type schema:Organization
    215 https://www.grid.ac/institutes/grid.31501.36 schema:alternateName Seoul National University
    216 schema:name School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, 151-742, Seoul, Republic of Korea
    217 rdf:type schema:Organization
    218 https://www.grid.ac/institutes/grid.411203.5 schema:alternateName Kyonggi University
    219 schema:name Department of Food Science and Biotechnology, Kyonggi University, 443-760, Suwon, Republic of Korea
    220 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...