Cross-linking of structural proteins in ageing skin: an in situ assay for the detection of amine oxidase activity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-02

AUTHORS

Abigail K. Langton, Christopher E. M. Griffiths, Michael J. Sherratt, Rachel E. B. Watson

ABSTRACT

With increasing age, dynamic tissues such as lungs, blood vessels and skin lose their ability to both deform and recoil, culminating in tissue stiffening. This loss of tissue elasticity, which profoundly impacts tissue function and thus morbidity, may be due not only to changes in the relative abundance of key extracellular matrix proteins within tissues but also to their accumulation of post-translational modifications. Whilst to date attention has focussed primarily on the age-related non-enzymatic formation of advanced glycation end products, the accumulation of pathological enzyme-mediated cross-links may also lead to age-related tissue stiffening. The lysyl oxidase (LOX) family of enzymes are constitutively expressed in adult tissues and are known to drive the catalysis of cross-links in both fibrillar collagens and elastin. Although immunochemical approaches are commonly used to localise the inactive pro-enzyme of LOX, and biochemical methods are employed to quantify activity in homogenised tissue, they do not allow for the in situ localisation of the enzyme. Thus, we have developed a novel assay to both detect and localise LOX enzyme activity in situ. LOX family members are amine oxidases and this assay uses the principle that an amine substrate in the presence of this class of enzyme will be oxidised to an aldehyde and hydrogen peroxide (H2O2). In turn, H2O2, when combined with luminol and horseradish peroxidase, will produce a light-emitting reaction that can be detected by film autoradiography. The development of a technique to localise specific amine oxidase activity in tissue sections may provide crucial additional information on the exact role played by this class of enzymes in mediating age-related tissue stiffening. More... »

PAGES

89-97

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10522-012-9394-3

DOI

http://dx.doi.org/10.1007/s10522-012-9394-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008579171

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22923173


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adolescent", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Oxidoreductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cross-Linking Reagents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Elastin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Extracellular Matrix Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibrillar Collagens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein-Lysine 6-Oxidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Skin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Skin Aging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Young Adult", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Manchester", 
          "id": "https://www.grid.ac/institutes/grid.5379.8", 
          "name": [
            "Dermatology Research Centre, Institute of Inflammation and Repair, Manchester Academic Health Science Centre, The University of Manchester, 1.523 Stopford Building, Oxford Road, M13 9PT, Manchester, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Langton", 
        "givenName": "Abigail K.", 
        "id": "sg:person.01264736667.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264736667.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Manchester", 
          "id": "https://www.grid.ac/institutes/grid.5379.8", 
          "name": [
            "Dermatology Research Centre, Institute of Inflammation and Repair, Manchester Academic Health Science Centre, The University of Manchester, 1.523 Stopford Building, Oxford Road, M13 9PT, Manchester, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Griffiths", 
        "givenName": "Christopher E. M.", 
        "id": "sg:person.07704176017.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07704176017.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Manchester", 
          "id": "https://www.grid.ac/institutes/grid.5379.8", 
          "name": [
            "Dermatology Research Centre, Institute of Inflammation and Repair, Manchester Academic Health Science Centre, The University of Manchester, 1.523 Stopford Building, Oxford Road, M13 9PT, Manchester, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sherratt", 
        "givenName": "Michael J.", 
        "id": "sg:person.01320347756.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320347756.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Manchester", 
          "id": "https://www.grid.ac/institutes/grid.5379.8", 
          "name": [
            "Dermatology Research Centre, Institute of Inflammation and Repair, Manchester Academic Health Science Centre, The University of Manchester, 1.523 Stopford Building, Oxford Road, M13 9PT, Manchester, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watson", 
        "givenName": "Rachel E. B.", 
        "id": "sg:person.016651732002.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016651732002.57"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1600-0714.1999.tb02033.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000429542"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0714.1999.tb02033.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000429542"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1987.tb03390.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004214000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1987.tb03390.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004214000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/j.0022-202x.2004.23436.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004269619", 
          "https://doi.org/10.1111/j.0022-202x.2004.23436.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0079-6603(01)70012-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005288123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00011-006-0526-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006141708", 
          "https://doi.org/10.1007/s00011-006-0526-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg753", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006818470", 
          "https://doi.org/10.1038/nrg753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg753", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006818470", 
          "https://doi.org/10.1038/nrg753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0001-706x(00)00128-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006941074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/j.0022-202x.2004.22330.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010683652", 
          "https://doi.org/10.1111/j.0022-202x.2004.22330.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/ajrcmb/5.3.206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013492777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2300639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013744164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2300639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013744164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11357-009-9103-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016602286", 
          "https://doi.org/10.1007/s11357-009-9103-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11357-009-9103-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016602286", 
          "https://doi.org/10.1007/s11357-009-9103-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07216", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018044273", 
          "https://doi.org/10.1038/nature07216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/japplphysiol.90549.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018632060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1995.0001f.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019328472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/djq569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020738739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10522-011-9332-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021451166", 
          "https://doi.org/10.1007/s10522-011-9332-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268874", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022468919", 
          "https://doi.org/10.1007/bf00268874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268874", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022468919", 
          "https://doi.org/10.1007/bf00268874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci115204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027262920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1010048014925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027804288", 
          "https://doi.org/10.1023/a:1010048014925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci104030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031080070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpheart.00335.2010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032547095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1167/iovs.07-1550", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034400052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/03008208909016817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034838865"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.2001.5464", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035020023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1357-2725(96)00079-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035150145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(96)01245-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036559180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-363708-6.50009-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037240386"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1570-9639(03)00053-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041942164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0047-6374(01)00225-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042448185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12280259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045042851", 
          "https://doi.org/10.1111/1523-1747.ep12280259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m006700200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047251272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02658498", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047363285", 
          "https://doi.org/10.1007/bf02658498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1113/jphysiol.1953.sp005012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048667832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12507866", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048689378", 
          "https://doi.org/10.1111/1523-1747.ep12507866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1113/jphysiol.1955.sp005370", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050503990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m709885200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052460541"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001250051342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053255265", 
          "https://doi.org/10.1007/s001250051342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001250051342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053255265", 
          "https://doi.org/10.1007/s001250051342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/jappl.2000.89.1.163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074670955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075075782", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075778217", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079963105", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082466602", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/b103823", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085722916", 
          "https://doi.org/10.1007/b103823"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-02", 
    "datePublishedReg": "2013-02-01", 
    "description": "With increasing age, dynamic tissues such as lungs, blood vessels and skin lose their ability to both deform and recoil, culminating in tissue stiffening. This loss of tissue elasticity, which profoundly impacts tissue function and thus morbidity, may be due not only to changes in the relative abundance of key extracellular matrix proteins within tissues but also to their accumulation of post-translational modifications. Whilst to date attention has focussed primarily on the age-related non-enzymatic formation of advanced glycation end products, the accumulation of pathological enzyme-mediated cross-links may also lead to age-related tissue stiffening. The lysyl oxidase (LOX) family of enzymes are constitutively expressed in adult tissues and are known to drive the catalysis of cross-links in both fibrillar collagens and elastin. Although immunochemical approaches are commonly used to localise the inactive pro-enzyme of LOX, and biochemical methods are employed to quantify activity in homogenised tissue, they do not allow for the in situ localisation of the enzyme. Thus, we have developed a novel assay to both detect and localise LOX enzyme activity in situ. LOX family members are amine oxidases and this assay uses the principle that an amine substrate in the presence of this class of enzyme will be oxidised to an aldehyde and hydrogen peroxide (H2O2). In turn, H2O2, when combined with luminol and horseradish peroxidase, will produce a light-emitting reaction that can be detected by film autoradiography. The development of a technique to localise specific amine oxidase activity in tissue sections may provide crucial additional information on the exact role played by this class of enzymes in mediating age-related tissue stiffening.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10522-012-9394-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1022481", 
        "issn": [
          "1389-5729", 
          "1573-6768"
        ], 
        "name": "Biogerontology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "14"
      }
    ], 
    "name": "Cross-linking of structural proteins in ageing skin: an in situ assay for the detection of amine oxidase activity", 
    "pagination": "89-97", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "28df72f8713257d866f5adf1abd45b9e7a33287fac28a5c68e4b80d21b3692d4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22923173"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100930043"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10522-012-9394-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1008579171"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10522-012-9394-3", 
      "https://app.dimensions.ai/details/publication/pub.1008579171"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:46", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000510.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs10522-012-9394-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10522-012-9394-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10522-012-9394-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10522-012-9394-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10522-012-9394-3'


 

This table displays all metadata directly associated to this object as RDF triples.

285 TRIPLES      21 PREDICATES      86 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10522-012-9394-3 schema:about N0200de0bc1574a1383dbbe39fa621980
2 N2bc33972498a4584851c014a6e29d3c0
3 N3ad45f0e365841f49f588ecab5b61b70
4 N426288936d7d448999579418f67e9201
5 N622e51c80a354f34a481560744bfd41b
6 N651579f4a7854f569690e947a7da7462
7 N9d5b06675e3241979ddfdd1e8f46f8a8
8 N9dfd67d40ac04524afc314e9b6fb25ce
9 Nbbe7b3443f0841c7826e445394cf4c64
10 Nbd59a53dafdf4324ab7448d2340dda55
11 Nd0a075f0bf6d456688ebf2b738a917f2
12 Nd43c4f73ce0140479a72ae51f12acb5f
13 Ndd9fbdb989424c30b0871e40fd562540
14 Nf3a7b3366fe84133b2c144fe68bbe678
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N52907346e1744370a8cab471e1cb5d6a
18 schema:citation sg:pub.10.1007/b103823
19 sg:pub.10.1007/bf00268874
20 sg:pub.10.1007/bf02658498
21 sg:pub.10.1007/s00011-006-0526-6
22 sg:pub.10.1007/s001250051342
23 sg:pub.10.1007/s10522-011-9332-9
24 sg:pub.10.1007/s11357-009-9103-6
25 sg:pub.10.1023/a:1010048014925
26 sg:pub.10.1038/nature07216
27 sg:pub.10.1038/nrg753
28 sg:pub.10.1111/1523-1747.ep12280259
29 sg:pub.10.1111/1523-1747.ep12507866
30 sg:pub.10.1111/j.0022-202x.2004.22330.x
31 sg:pub.10.1111/j.0022-202x.2004.23436.x
32 https://app.dimensions.ai/details/publication/pub.1075075782
33 https://app.dimensions.ai/details/publication/pub.1075778217
34 https://app.dimensions.ai/details/publication/pub.1079963105
35 https://app.dimensions.ai/details/publication/pub.1082466602
36 https://doi.org/10.1006/abio.2001.5464
37 https://doi.org/10.1016/b978-0-12-363708-6.50009-6
38 https://doi.org/10.1016/s0001-706x(00)00128-5
39 https://doi.org/10.1016/s0014-5793(96)01245-8
40 https://doi.org/10.1016/s0047-6374(01)00225-1
41 https://doi.org/10.1016/s0079-6603(01)70012-8
42 https://doi.org/10.1016/s1357-2725(96)00079-9
43 https://doi.org/10.1016/s1570-9639(03)00053-0
44 https://doi.org/10.1042/bj2300639
45 https://doi.org/10.1074/jbc.m006700200
46 https://doi.org/10.1074/jbc.m709885200
47 https://doi.org/10.1093/jnci/djq569
48 https://doi.org/10.1111/j.1399-3011.1987.tb03390.x
49 https://doi.org/10.1111/j.1432-1033.1995.0001f.x
50 https://doi.org/10.1111/j.1600-0714.1999.tb02033.x
51 https://doi.org/10.1113/jphysiol.1953.sp005012
52 https://doi.org/10.1113/jphysiol.1955.sp005370
53 https://doi.org/10.1152/ajpheart.00335.2010
54 https://doi.org/10.1152/jappl.2000.89.1.163
55 https://doi.org/10.1152/japplphysiol.90549.2008
56 https://doi.org/10.1165/ajrcmb/5.3.206
57 https://doi.org/10.1167/iovs.07-1550
58 https://doi.org/10.1172/jci104030
59 https://doi.org/10.1172/jci115204
60 https://doi.org/10.3109/03008208909016817
61 schema:datePublished 2013-02
62 schema:datePublishedReg 2013-02-01
63 schema:description With increasing age, dynamic tissues such as lungs, blood vessels and skin lose their ability to both deform and recoil, culminating in tissue stiffening. This loss of tissue elasticity, which profoundly impacts tissue function and thus morbidity, may be due not only to changes in the relative abundance of key extracellular matrix proteins within tissues but also to their accumulation of post-translational modifications. Whilst to date attention has focussed primarily on the age-related non-enzymatic formation of advanced glycation end products, the accumulation of pathological enzyme-mediated cross-links may also lead to age-related tissue stiffening. The lysyl oxidase (LOX) family of enzymes are constitutively expressed in adult tissues and are known to drive the catalysis of cross-links in both fibrillar collagens and elastin. Although immunochemical approaches are commonly used to localise the inactive pro-enzyme of LOX, and biochemical methods are employed to quantify activity in homogenised tissue, they do not allow for the in situ localisation of the enzyme. Thus, we have developed a novel assay to both detect and localise LOX enzyme activity in situ. LOX family members are amine oxidases and this assay uses the principle that an amine substrate in the presence of this class of enzyme will be oxidised to an aldehyde and hydrogen peroxide (H2O2). In turn, H2O2, when combined with luminol and horseradish peroxidase, will produce a light-emitting reaction that can be detected by film autoradiography. The development of a technique to localise specific amine oxidase activity in tissue sections may provide crucial additional information on the exact role played by this class of enzymes in mediating age-related tissue stiffening.
64 schema:genre research_article
65 schema:inLanguage en
66 schema:isAccessibleForFree false
67 schema:isPartOf N1fd78837652d489cb9775b5c2e989938
68 N21175be90d1843db998c79d25120eae6
69 sg:journal.1022481
70 schema:name Cross-linking of structural proteins in ageing skin: an in situ assay for the detection of amine oxidase activity
71 schema:pagination 89-97
72 schema:productId N240e1ffdb4e54f648344d0407c48e70b
73 N57bb1da2623b415c8b762f41f61984df
74 N8c3b12b510244addab43bd5ba48c4f59
75 Nca69937aeb56412c93811e9e4de95f5e
76 Nffbd7216a4ea4185ad4a2532ec6c10c8
77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008579171
78 https://doi.org/10.1007/s10522-012-9394-3
79 schema:sdDatePublished 2019-04-10T20:46
80 schema:sdLicense https://scigraph.springernature.com/explorer/license/
81 schema:sdPublisher N21515bd2076e4aabbf07541f1fb11495
82 schema:url http://link.springer.com/10.1007%2Fs10522-012-9394-3
83 sgo:license sg:explorer/license/
84 sgo:sdDataset articles
85 rdf:type schema:ScholarlyArticle
86 N0200de0bc1574a1383dbbe39fa621980 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Amino Acid Oxidoreductases
88 rdf:type schema:DefinedTerm
89 N1fd78837652d489cb9775b5c2e989938 schema:volumeNumber 14
90 rdf:type schema:PublicationVolume
91 N21175be90d1843db998c79d25120eae6 schema:issueNumber 1
92 rdf:type schema:PublicationIssue
93 N21515bd2076e4aabbf07541f1fb11495 schema:name Springer Nature - SN SciGraph project
94 rdf:type schema:Organization
95 N240e1ffdb4e54f648344d0407c48e70b schema:name doi
96 schema:value 10.1007/s10522-012-9394-3
97 rdf:type schema:PropertyValue
98 N2bc33972498a4584851c014a6e29d3c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Protein-Lysine 6-Oxidase
100 rdf:type schema:DefinedTerm
101 N31d02a0751fe4b22b8a85171f0a0c17d rdf:first sg:person.016651732002.57
102 rdf:rest rdf:nil
103 N3ad45f0e365841f49f588ecab5b61b70 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Young Adult
105 rdf:type schema:DefinedTerm
106 N426288936d7d448999579418f67e9201 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Skin Aging
108 rdf:type schema:DefinedTerm
109 N52907346e1744370a8cab471e1cb5d6a rdf:first sg:person.01264736667.47
110 rdf:rest Na620a4ba03f9457ea93471af13b6fef6
111 N57bb1da2623b415c8b762f41f61984df schema:name readcube_id
112 schema:value 28df72f8713257d866f5adf1abd45b9e7a33287fac28a5c68e4b80d21b3692d4
113 rdf:type schema:PropertyValue
114 N594514d6c65241a99f32d4f2f548845b rdf:first sg:person.01320347756.05
115 rdf:rest N31d02a0751fe4b22b8a85171f0a0c17d
116 N622e51c80a354f34a481560744bfd41b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Aged
118 rdf:type schema:DefinedTerm
119 N651579f4a7854f569690e947a7da7462 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Skin
121 rdf:type schema:DefinedTerm
122 N8c3b12b510244addab43bd5ba48c4f59 schema:name pubmed_id
123 schema:value 22923173
124 rdf:type schema:PropertyValue
125 N9d5b06675e3241979ddfdd1e8f46f8a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Humans
127 rdf:type schema:DefinedTerm
128 N9dfd67d40ac04524afc314e9b6fb25ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Cross-Linking Reagents
130 rdf:type schema:DefinedTerm
131 Na620a4ba03f9457ea93471af13b6fef6 rdf:first sg:person.07704176017.63
132 rdf:rest N594514d6c65241a99f32d4f2f548845b
133 Nbbe7b3443f0841c7826e445394cf4c64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Fibrillar Collagens
135 rdf:type schema:DefinedTerm
136 Nbd59a53dafdf4324ab7448d2340dda55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Extracellular Matrix Proteins
138 rdf:type schema:DefinedTerm
139 Nca69937aeb56412c93811e9e4de95f5e schema:name nlm_unique_id
140 schema:value 100930043
141 rdf:type schema:PropertyValue
142 Nd0a075f0bf6d456688ebf2b738a917f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Adult
144 rdf:type schema:DefinedTerm
145 Nd43c4f73ce0140479a72ae51f12acb5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Adolescent
147 rdf:type schema:DefinedTerm
148 Ndd9fbdb989424c30b0871e40fd562540 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Substrate Specificity
150 rdf:type schema:DefinedTerm
151 Nf3a7b3366fe84133b2c144fe68bbe678 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Elastin
153 rdf:type schema:DefinedTerm
154 Nffbd7216a4ea4185ad4a2532ec6c10c8 schema:name dimensions_id
155 schema:value pub.1008579171
156 rdf:type schema:PropertyValue
157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biological Sciences
159 rdf:type schema:DefinedTerm
160 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
161 schema:name Biochemistry and Cell Biology
162 rdf:type schema:DefinedTerm
163 sg:journal.1022481 schema:issn 1389-5729
164 1573-6768
165 schema:name Biogerontology
166 rdf:type schema:Periodical
167 sg:person.01264736667.47 schema:affiliation https://www.grid.ac/institutes/grid.5379.8
168 schema:familyName Langton
169 schema:givenName Abigail K.
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264736667.47
171 rdf:type schema:Person
172 sg:person.01320347756.05 schema:affiliation https://www.grid.ac/institutes/grid.5379.8
173 schema:familyName Sherratt
174 schema:givenName Michael J.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320347756.05
176 rdf:type schema:Person
177 sg:person.016651732002.57 schema:affiliation https://www.grid.ac/institutes/grid.5379.8
178 schema:familyName Watson
179 schema:givenName Rachel E. B.
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016651732002.57
181 rdf:type schema:Person
182 sg:person.07704176017.63 schema:affiliation https://www.grid.ac/institutes/grid.5379.8
183 schema:familyName Griffiths
184 schema:givenName Christopher E. M.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07704176017.63
186 rdf:type schema:Person
187 sg:pub.10.1007/b103823 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085722916
188 https://doi.org/10.1007/b103823
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/bf00268874 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022468919
191 https://doi.org/10.1007/bf00268874
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/bf02658498 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047363285
194 https://doi.org/10.1007/bf02658498
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/s00011-006-0526-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006141708
197 https://doi.org/10.1007/s00011-006-0526-6
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/s001250051342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053255265
200 https://doi.org/10.1007/s001250051342
201 rdf:type schema:CreativeWork
202 sg:pub.10.1007/s10522-011-9332-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021451166
203 https://doi.org/10.1007/s10522-011-9332-9
204 rdf:type schema:CreativeWork
205 sg:pub.10.1007/s11357-009-9103-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016602286
206 https://doi.org/10.1007/s11357-009-9103-6
207 rdf:type schema:CreativeWork
208 sg:pub.10.1023/a:1010048014925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027804288
209 https://doi.org/10.1023/a:1010048014925
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/nature07216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018044273
212 https://doi.org/10.1038/nature07216
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/nrg753 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006818470
215 https://doi.org/10.1038/nrg753
216 rdf:type schema:CreativeWork
217 sg:pub.10.1111/1523-1747.ep12280259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045042851
218 https://doi.org/10.1111/1523-1747.ep12280259
219 rdf:type schema:CreativeWork
220 sg:pub.10.1111/1523-1747.ep12507866 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048689378
221 https://doi.org/10.1111/1523-1747.ep12507866
222 rdf:type schema:CreativeWork
223 sg:pub.10.1111/j.0022-202x.2004.22330.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010683652
224 https://doi.org/10.1111/j.0022-202x.2004.22330.x
225 rdf:type schema:CreativeWork
226 sg:pub.10.1111/j.0022-202x.2004.23436.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004269619
227 https://doi.org/10.1111/j.0022-202x.2004.23436.x
228 rdf:type schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1075075782 schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1075778217 schema:CreativeWork
231 https://app.dimensions.ai/details/publication/pub.1079963105 schema:CreativeWork
232 https://app.dimensions.ai/details/publication/pub.1082466602 schema:CreativeWork
233 https://doi.org/10.1006/abio.2001.5464 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035020023
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/b978-0-12-363708-6.50009-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037240386
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0001-706x(00)00128-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006941074
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0014-5793(96)01245-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036559180
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s0047-6374(01)00225-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042448185
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0079-6603(01)70012-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005288123
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/s1357-2725(96)00079-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035150145
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/s1570-9639(03)00053-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041942164
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1042/bj2300639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013744164
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1074/jbc.m006700200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047251272
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1074/jbc.m709885200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052460541
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1093/jnci/djq569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020738739
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1111/j.1399-3011.1987.tb03390.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004214000
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1111/j.1432-1033.1995.0001f.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019328472
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1111/j.1600-0714.1999.tb02033.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000429542
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1113/jphysiol.1953.sp005012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048667832
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1113/jphysiol.1955.sp005370 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050503990
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1152/ajpheart.00335.2010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032547095
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1152/jappl.2000.89.1.163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074670955
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1152/japplphysiol.90549.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018632060
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1165/ajrcmb/5.3.206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013492777
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1167/iovs.07-1550 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034400052
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1172/jci104030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031080070
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1172/jci115204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027262920
280 rdf:type schema:CreativeWork
281 https://doi.org/10.3109/03008208909016817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034838865
282 rdf:type schema:CreativeWork
283 https://www.grid.ac/institutes/grid.5379.8 schema:alternateName University of Manchester
284 schema:name Dermatology Research Centre, Institute of Inflammation and Repair, Manchester Academic Health Science Centre, The University of Manchester, 1.523 Stopford Building, Oxford Road, M13 9PT, Manchester, UK
285 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...