Isolation of Induced Pluripotent Cells from Stromal Liver Cells of Patients with Alcoholic Cirrhosis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-08

AUTHORS

I. V. Kholodenko, R. V. Kholodenko, G. V. Manukyan, A. Yu. Lupatov, K. N. Yarygin

ABSTRACT

Stromal liver cells obtained from liver biopsy specimens of a patient with alcoholic cirrhosis can proliferate for a long time in culture passing more than 30 passages. In the course of culturing from early to late passages, acceleration of cell proliferation, decrease of the expression of some markers, and loss of hepatogenic differentiation potential were observed. On passage 30, induced pluripotent stem cells were obtained from these cells and comparative analysis of adipogenic and hepatic differentiation potencies of these cells and original liver stromal cells was performed. Induced pluripotent stem cells differentiated into both directions more efficiently and more rapidly than initial cells. Under conditions of hepatic differentiation, liver stromal cells started to express markers of definitive endoderm, but not markers of immature/mature hepatocytes, whereas induced pluripotent stem cells consistently expressed markers of definitive endoderm, immature/mature hepatocytes. More... »

PAGES

535-541

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10517-017-3845-4

DOI

http://dx.doi.org/10.1007/s10517-017-3845-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091378170

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28853085


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