Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-11

AUTHORS

A. S. Vdovin, A. Yu. Lupatov, I. V. Kholodenko, K. N. Yarygin

ABSTRACT

Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60. More... »

PAGES

123-128

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10517-015-3112-5

DOI

http://dx.doi.org/10.1007/s10517-015-3112-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025600603

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26593412


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