GD2 ganglioside-binding antibody 14G2a and specific aurora A kinase inhibitor MK-5108 induce autophagy in IMR-32 neuroblastoma cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-10

AUTHORS

Małgorzata Durbas, Paweł Pabisz, Katarzyna Wawak, Aneta Wiśniewska, Elżbieta Boratyn, Iwona Nowak, Irena Horwacik, Olga Woźnicka, Hanna Rokita

ABSTRACT

The process of autophagy and its role in survival of human neuroblastoma cell cultures was studied upon addition of an anti-GD2 ganglioside (GD2) 14G2a mouse monoclonal antibody (14G2a mAb) and an aurora A kinase specific inhibitor, MK-5108. It was recently shown that combination of these agents significantly potentiates cytotoxicity against IMR-32 and CHP-134 neuroblastoma cells in vitro, as compared to the inhibitor used alone. In this study we gained mechanistic insights on autophagy in the observed cytotoxic effects exerted by both agents using cytotoxicity assays, RT-qPCR, immunoblotting, and autophagy detection methods. Enhancement of the autophagy process in the 14G2a mAb- and MK-5108-treated IMR-32 cells was documented by assessing autophagic flux. Application of a lysosomotropic agent-chloroquine (CQ) affected the 14G2a mAb- and MK-5108-stimulated autophagic flux. It is our conclusion that the 14G2a mAb (40 μg/ml) and MK-5108 inhibitor (0.1 μM) induce autophagy in IMR-32 cells. Moreover, the combinatorial treatment of IMR-32 cells with the 14G2a mAb and CQ significantly potentiates cytotoxic effect, as compared to CQ used alone. Most importantly, we showed that interfering with autophagy at its early and late step augments the 14G2a mAb-induced apoptosis, therefore we can conclude that inhibition of autophagy is the primary mechanism of the CQ-mediated sensitization to the 14G2a mAb-induced apoptosis. Although, there was no virtual stimulation of autophagy in the 14G2a mAb-treated CHP-134 neuroblastoma cells, we were able to show that PHLDA1 protein positively regulates autophagy and this process exists in a mutually exclusive manner with apoptosis in PHLDA1-silenced CHP-134 cells. More... »

PAGES

492-511

Journal

TITLE

Apoptosis

ISSUE

9-10

VOLUME

23

Author Affiliations

From Grant

  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10495-018-1472-9

    DOI

    http://dx.doi.org/10.1007/s10495-018-1472-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1105710208

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30027525


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Durbas", 
            "givenName": "Ma\u0142gorzata", 
            "id": "sg:person.0642667060.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0642667060.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Departament of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pabisz", 
            "givenName": "Pawe\u0142", 
            "id": "sg:person.0744060664.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744060664.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Departament of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wawak", 
            "givenName": "Katarzyna", 
            "id": "sg:person.0675745464.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675745464.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wi\u015bniewska", 
            "givenName": "Aneta", 
            "id": "sg:person.011232441260.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011232441260.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Boratyn", 
            "givenName": "El\u017cbieta", 
            "id": "sg:person.0711002260.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0711002260.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nowak", 
            "givenName": "Iwona", 
            "id": "sg:person.01247342265.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247342265.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Horwacik", 
            "givenName": "Irena", 
            "id": "sg:person.0633172760.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633172760.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Department of Cell Biology and Imaging, Faculty of Biology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wo\u017anicka", 
            "givenName": "Olga", 
            "id": "sg:person.01205133071.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205133071.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jagiellonian University", 
              "id": "https://www.grid.ac/institutes/grid.5522.0", 
              "name": [
                "Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krak\u00f3w, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rokita", 
            "givenName": "Hanna", 
            "id": "sg:person.01245735230.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245735230.04"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1371/journal.pone.0078958", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001256500"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0078958", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001256500"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0078958", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001256500"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.03.048", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001834748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2010.01.028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001921233"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.canlet.2013.08.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004263845"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cddis.2012.13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005869496", 
              "https://doi.org/10.1038/cddis.2012.13"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1182/blood-2006-10-050260", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007065383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008818340", 
              "https://doi.org/10.1038/nrc1014"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008818340", 
              "https://doi.org/10.1038/nrc1014"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ccr.2008.12.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009919594"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.canlet.2009.02.040", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014563742"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1208095", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016994408", 
              "https://doi.org/10.1038/sj.onc.1208095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1208095", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016994408", 
              "https://doi.org/10.1038/sj.onc.1208095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1744-8069-7-83", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020084738", 
              "https://doi.org/10.1186/1744-8069-7-83"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrclinonc.2014.168", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021199341", 
              "https://doi.org/10.1038/nrclinonc.2014.168"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/onc.2008.310", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024186435", 
              "https://doi.org/10.1038/onc.2008.310"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biocel.2004.02.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026516637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0408345102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027928976"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1074-7613(00)80484-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027949568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s40265-015-0399-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028838509", 
              "https://doi.org/10.1007/s40265-015-0399-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ejphar.2009.06.063", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028874552"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ctrv.2010.02.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029242131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2199-15-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031548883", 
              "https://doi.org/10.1186/1471-2199-15-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12013-012-9504-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032273076", 
              "https://doi.org/10.1007/s12013-012-9504-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2147/dddt.s75378", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032781404"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.18632/oncotarget.6778", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032894470"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/15548627.2015.1017181", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033545432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1738", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034470904", 
              "https://doi.org/10.1038/nrc1738"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1738", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034470904", 
              "https://doi.org/10.1038/nrc1738"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fonc.2015.00278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035106163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ccr.2006.06.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035424320"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-03-3084", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036595848"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/theoncologist.2009-0019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040868980"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-07-5023", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041626084"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m111.257519", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043885062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci35512", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046185955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1535-7163.mct-11-0047", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047209691"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.devcel.2009.12.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048092082"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/scisignal.2001017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062682214"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/br.2016.580", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071502583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ijo.2015.3070", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071513983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ijo.2015.3070", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071513983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/ijo.2016.3572", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071514414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/auto.1.2.1697", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072289414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/auto.22110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072289631"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/auto.27959", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072289977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/auto.4600", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072290239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/auto.6732", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072290872"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074816033", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ejmech.2017.08.045", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091326671"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.18632/oncotarget.22328", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092591192"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-10", 
        "datePublishedReg": "2018-10-01", 
        "description": "The process of autophagy and its role in survival of human neuroblastoma cell cultures was studied upon addition of an anti-GD2 ganglioside (GD2) 14G2a mouse monoclonal antibody (14G2a mAb) and an aurora A kinase specific inhibitor, MK-5108. It was recently shown that combination of these agents significantly potentiates cytotoxicity against IMR-32 and CHP-134 neuroblastoma cells in vitro, as compared to the inhibitor used alone. In this study we gained mechanistic insights on autophagy in the observed cytotoxic effects exerted by both agents using cytotoxicity assays, RT-qPCR, immunoblotting, and autophagy detection methods. Enhancement of the autophagy process in the 14G2a mAb- and MK-5108-treated IMR-32 cells was documented by assessing autophagic flux. Application of a lysosomotropic agent-chloroquine (CQ) affected the 14G2a mAb- and MK-5108-stimulated autophagic flux. It is our conclusion that the 14G2a mAb (40\u00a0\u03bcg/ml) and MK-5108 inhibitor (0.1\u00a0\u03bcM) induce autophagy in IMR-32 cells. Moreover, the combinatorial treatment of IMR-32 cells with the 14G2a mAb and CQ significantly potentiates cytotoxic effect, as compared to CQ used alone. Most importantly, we showed that interfering with autophagy at its early and late step augments the 14G2a mAb-induced apoptosis, therefore we can conclude that inhibition of autophagy is the primary mechanism of the CQ-mediated sensitization to the 14G2a mAb-induced apoptosis. Although, there was no virtual stimulation of autophagy in the 14G2a mAb-treated CHP-134 neuroblastoma cells, we were able to show that PHLDA1 protein positively regulates autophagy and this process exists in a mutually exclusive manner with apoptosis in PHLDA1-silenced CHP-134 cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s10495-018-1472-9", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.7399612", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.7404626", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1117236", 
            "issn": [
              "1360-8185", 
              "1573-675X"
            ], 
            "name": "Apoptosis", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "9-10", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "23"
          }
        ], 
        "name": "GD2 ganglioside-binding antibody 14G2a and specific aurora A kinase inhibitor MK-5108 induce autophagy in IMR-32 neuroblastoma cells", 
        "pagination": "492-511", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "c29314ccd1799df5efff8853d3cb90bab0e2015891926f11ea64d8c6851846a1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30027525"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9712129"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10495-018-1472-9"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1105710208"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10495-018-1472-9", 
          "https://app.dimensions.ai/details/publication/pub.1105710208"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:50", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000560.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs10495-018-1472-9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10495-018-1472-9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10495-018-1472-9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10495-018-1472-9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10495-018-1472-9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    278 TRIPLES      21 PREDICATES      75 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10495-018-1472-9 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author Nf924f66fea6b47f79f88e213266e83c4
    4 schema:citation sg:pub.10.1007/s12013-012-9504-5
    5 sg:pub.10.1007/s40265-015-0399-5
    6 sg:pub.10.1038/cddis.2012.13
    7 sg:pub.10.1038/nrc1014
    8 sg:pub.10.1038/nrc1738
    9 sg:pub.10.1038/nrclinonc.2014.168
    10 sg:pub.10.1038/onc.2008.310
    11 sg:pub.10.1038/sj.onc.1208095
    12 sg:pub.10.1186/1471-2199-15-9
    13 sg:pub.10.1186/1744-8069-7-83
    14 https://app.dimensions.ai/details/publication/pub.1074816033
    15 https://doi.org/10.1016/j.biocel.2004.02.002
    16 https://doi.org/10.1016/j.canlet.2009.02.040
    17 https://doi.org/10.1016/j.canlet.2013.08.018
    18 https://doi.org/10.1016/j.ccr.2006.06.001
    19 https://doi.org/10.1016/j.ccr.2008.12.005
    20 https://doi.org/10.1016/j.cell.2009.03.048
    21 https://doi.org/10.1016/j.cell.2010.01.028
    22 https://doi.org/10.1016/j.ctrv.2010.02.006
    23 https://doi.org/10.1016/j.devcel.2009.12.004
    24 https://doi.org/10.1016/j.ejmech.2017.08.045
    25 https://doi.org/10.1016/j.ejphar.2009.06.063
    26 https://doi.org/10.1016/s1074-7613(00)80484-7
    27 https://doi.org/10.1073/pnas.0408345102
    28 https://doi.org/10.1074/jbc.m111.257519
    29 https://doi.org/10.1080/15548627.2015.1017181
    30 https://doi.org/10.1126/scisignal.2001017
    31 https://doi.org/10.1158/0008-5472.can-03-3084
    32 https://doi.org/10.1158/1078-0432.ccr-07-5023
    33 https://doi.org/10.1158/1535-7163.mct-11-0047
    34 https://doi.org/10.1172/jci35512
    35 https://doi.org/10.1182/blood-2006-10-050260
    36 https://doi.org/10.1371/journal.pone.0078958
    37 https://doi.org/10.1634/theoncologist.2009-0019
    38 https://doi.org/10.18632/oncotarget.22328
    39 https://doi.org/10.18632/oncotarget.6778
    40 https://doi.org/10.2147/dddt.s75378
    41 https://doi.org/10.3389/fonc.2015.00278
    42 https://doi.org/10.3892/br.2016.580
    43 https://doi.org/10.3892/ijo.2015.3070
    44 https://doi.org/10.3892/ijo.2016.3572
    45 https://doi.org/10.4161/auto.1.2.1697
    46 https://doi.org/10.4161/auto.22110
    47 https://doi.org/10.4161/auto.27959
    48 https://doi.org/10.4161/auto.4600
    49 https://doi.org/10.4161/auto.6732
    50 schema:datePublished 2018-10
    51 schema:datePublishedReg 2018-10-01
    52 schema:description The process of autophagy and its role in survival of human neuroblastoma cell cultures was studied upon addition of an anti-GD2 ganglioside (GD2) 14G2a mouse monoclonal antibody (14G2a mAb) and an aurora A kinase specific inhibitor, MK-5108. It was recently shown that combination of these agents significantly potentiates cytotoxicity against IMR-32 and CHP-134 neuroblastoma cells in vitro, as compared to the inhibitor used alone. In this study we gained mechanistic insights on autophagy in the observed cytotoxic effects exerted by both agents using cytotoxicity assays, RT-qPCR, immunoblotting, and autophagy detection methods. Enhancement of the autophagy process in the 14G2a mAb- and MK-5108-treated IMR-32 cells was documented by assessing autophagic flux. Application of a lysosomotropic agent-chloroquine (CQ) affected the 14G2a mAb- and MK-5108-stimulated autophagic flux. It is our conclusion that the 14G2a mAb (40 μg/ml) and MK-5108 inhibitor (0.1 μM) induce autophagy in IMR-32 cells. Moreover, the combinatorial treatment of IMR-32 cells with the 14G2a mAb and CQ significantly potentiates cytotoxic effect, as compared to CQ used alone. Most importantly, we showed that interfering with autophagy at its early and late step augments the 14G2a mAb-induced apoptosis, therefore we can conclude that inhibition of autophagy is the primary mechanism of the CQ-mediated sensitization to the 14G2a mAb-induced apoptosis. Although, there was no virtual stimulation of autophagy in the 14G2a mAb-treated CHP-134 neuroblastoma cells, we were able to show that PHLDA1 protein positively regulates autophagy and this process exists in a mutually exclusive manner with apoptosis in PHLDA1-silenced CHP-134 cells.
    53 schema:genre research_article
    54 schema:inLanguage en
    55 schema:isAccessibleForFree true
    56 schema:isPartOf N62866e1d50654cd485b4c9b1e49818c8
    57 N79fee7116367474d9264d86e1f069413
    58 sg:journal.1117236
    59 schema:name GD2 ganglioside-binding antibody 14G2a and specific aurora A kinase inhibitor MK-5108 induce autophagy in IMR-32 neuroblastoma cells
    60 schema:pagination 492-511
    61 schema:productId N091c8acff062488cb48e5b9b067a8142
    62 N3c926f5ad152402fb7835cb78349410f
    63 N4dc741290ee54baea630caf2992a55c6
    64 Nf80111cc0ec3480083228ca1a00ec82e
    65 Nfa6fcb093afd481aa966cdcf7ffd921a
    66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105710208
    67 https://doi.org/10.1007/s10495-018-1472-9
    68 schema:sdDatePublished 2019-04-10T16:50
    69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    70 schema:sdPublisher Nca92cf130d9e45e8b9ce4c68c96950bc
    71 schema:url https://link.springer.com/10.1007%2Fs10495-018-1472-9
    72 sgo:license sg:explorer/license/
    73 sgo:sdDataset articles
    74 rdf:type schema:ScholarlyArticle
    75 N091c8acff062488cb48e5b9b067a8142 schema:name doi
    76 schema:value 10.1007/s10495-018-1472-9
    77 rdf:type schema:PropertyValue
    78 N098e7284b70b47d5bb88cc8c2fdc4a0f rdf:first sg:person.011232441260.13
    79 rdf:rest N887bd9dc5c654356937cd3738bc2be3f
    80 N107b0c59bf364f91b4354ca941f5a614 rdf:first sg:person.0675745464.96
    81 rdf:rest N098e7284b70b47d5bb88cc8c2fdc4a0f
    82 N3c926f5ad152402fb7835cb78349410f schema:name readcube_id
    83 schema:value c29314ccd1799df5efff8853d3cb90bab0e2015891926f11ea64d8c6851846a1
    84 rdf:type schema:PropertyValue
    85 N4dc741290ee54baea630caf2992a55c6 schema:name dimensions_id
    86 schema:value pub.1105710208
    87 rdf:type schema:PropertyValue
    88 N62866e1d50654cd485b4c9b1e49818c8 schema:issueNumber 9-10
    89 rdf:type schema:PublicationIssue
    90 N7758e811623e49e1aaa6917562a01e82 rdf:first sg:person.01245735230.04
    91 rdf:rest rdf:nil
    92 N79f920ba096343ebac93ad910ff2f9ec rdf:first sg:person.01205133071.54
    93 rdf:rest N7758e811623e49e1aaa6917562a01e82
    94 N79fee7116367474d9264d86e1f069413 schema:volumeNumber 23
    95 rdf:type schema:PublicationVolume
    96 N887bd9dc5c654356937cd3738bc2be3f rdf:first sg:person.0711002260.70
    97 rdf:rest N952fc58cf0ac4a0e9b4a7f38454595c8
    98 N8f56114792204c75add09d6589453d7f rdf:first sg:person.0633172760.38
    99 rdf:rest N79f920ba096343ebac93ad910ff2f9ec
    100 N952fc58cf0ac4a0e9b4a7f38454595c8 rdf:first sg:person.01247342265.79
    101 rdf:rest N8f56114792204c75add09d6589453d7f
    102 Nca92cf130d9e45e8b9ce4c68c96950bc schema:name Springer Nature - SN SciGraph project
    103 rdf:type schema:Organization
    104 Nf511cde557ab4fa6bd550be710806df5 rdf:first sg:person.0744060664.82
    105 rdf:rest N107b0c59bf364f91b4354ca941f5a614
    106 Nf80111cc0ec3480083228ca1a00ec82e schema:name pubmed_id
    107 schema:value 30027525
    108 rdf:type schema:PropertyValue
    109 Nf924f66fea6b47f79f88e213266e83c4 rdf:first sg:person.0642667060.85
    110 rdf:rest Nf511cde557ab4fa6bd550be710806df5
    111 Nfa6fcb093afd481aa966cdcf7ffd921a schema:name nlm_unique_id
    112 schema:value 9712129
    113 rdf:type schema:PropertyValue
    114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    115 schema:name Biological Sciences
    116 rdf:type schema:DefinedTerm
    117 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    118 schema:name Biochemistry and Cell Biology
    119 rdf:type schema:DefinedTerm
    120 sg:grant.7399612 http://pending.schema.org/fundedItem sg:pub.10.1007/s10495-018-1472-9
    121 rdf:type schema:MonetaryGrant
    122 sg:grant.7404626 http://pending.schema.org/fundedItem sg:pub.10.1007/s10495-018-1472-9
    123 rdf:type schema:MonetaryGrant
    124 sg:journal.1117236 schema:issn 1360-8185
    125 1573-675X
    126 schema:name Apoptosis
    127 rdf:type schema:Periodical
    128 sg:person.011232441260.13 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    129 schema:familyName Wiśniewska
    130 schema:givenName Aneta
    131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011232441260.13
    132 rdf:type schema:Person
    133 sg:person.01205133071.54 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    134 schema:familyName Woźnicka
    135 schema:givenName Olga
    136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205133071.54
    137 rdf:type schema:Person
    138 sg:person.01245735230.04 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    139 schema:familyName Rokita
    140 schema:givenName Hanna
    141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245735230.04
    142 rdf:type schema:Person
    143 sg:person.01247342265.79 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    144 schema:familyName Nowak
    145 schema:givenName Iwona
    146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247342265.79
    147 rdf:type schema:Person
    148 sg:person.0633172760.38 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    149 schema:familyName Horwacik
    150 schema:givenName Irena
    151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633172760.38
    152 rdf:type schema:Person
    153 sg:person.0642667060.85 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    154 schema:familyName Durbas
    155 schema:givenName Małgorzata
    156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0642667060.85
    157 rdf:type schema:Person
    158 sg:person.0675745464.96 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    159 schema:familyName Wawak
    160 schema:givenName Katarzyna
    161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675745464.96
    162 rdf:type schema:Person
    163 sg:person.0711002260.70 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    164 schema:familyName Boratyn
    165 schema:givenName Elżbieta
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0711002260.70
    167 rdf:type schema:Person
    168 sg:person.0744060664.82 schema:affiliation https://www.grid.ac/institutes/grid.5522.0
    169 schema:familyName Pabisz
    170 schema:givenName Paweł
    171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744060664.82
    172 rdf:type schema:Person
    173 sg:pub.10.1007/s12013-012-9504-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032273076
    174 https://doi.org/10.1007/s12013-012-9504-5
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1007/s40265-015-0399-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028838509
    177 https://doi.org/10.1007/s40265-015-0399-5
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1038/cddis.2012.13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005869496
    180 https://doi.org/10.1038/cddis.2012.13
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1038/nrc1014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008818340
    183 https://doi.org/10.1038/nrc1014
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1038/nrc1738 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034470904
    186 https://doi.org/10.1038/nrc1738
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1038/nrclinonc.2014.168 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021199341
    189 https://doi.org/10.1038/nrclinonc.2014.168
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1038/onc.2008.310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024186435
    192 https://doi.org/10.1038/onc.2008.310
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1038/sj.onc.1208095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016994408
    195 https://doi.org/10.1038/sj.onc.1208095
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1186/1471-2199-15-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031548883
    198 https://doi.org/10.1186/1471-2199-15-9
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1186/1744-8069-7-83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020084738
    201 https://doi.org/10.1186/1744-8069-7-83
    202 rdf:type schema:CreativeWork
    203 https://app.dimensions.ai/details/publication/pub.1074816033 schema:CreativeWork
    204 https://doi.org/10.1016/j.biocel.2004.02.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026516637
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1016/j.canlet.2009.02.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014563742
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1016/j.canlet.2013.08.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004263845
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/j.ccr.2006.06.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035424320
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/j.ccr.2008.12.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009919594
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/j.cell.2009.03.048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001834748
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/j.cell.2010.01.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001921233
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1016/j.ctrv.2010.02.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029242131
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/j.devcel.2009.12.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048092082
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/j.ejmech.2017.08.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091326671
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1016/j.ejphar.2009.06.063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028874552
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1016/s1074-7613(00)80484-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027949568
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1073/pnas.0408345102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027928976
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1074/jbc.m111.257519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043885062
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1080/15548627.2015.1017181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033545432
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1126/scisignal.2001017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062682214
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1158/0008-5472.can-03-3084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036595848
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1158/1078-0432.ccr-07-5023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041626084
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1158/1535-7163.mct-11-0047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047209691
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1172/jci35512 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046185955
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1182/blood-2006-10-050260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007065383
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1371/journal.pone.0078958 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001256500
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1634/theoncologist.2009-0019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040868980
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.18632/oncotarget.22328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092591192
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.18632/oncotarget.6778 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032894470
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.2147/dddt.s75378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032781404
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.3389/fonc.2015.00278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035106163
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.3892/br.2016.580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071502583
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.3892/ijo.2015.3070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071513983
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.3892/ijo.2016.3572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071514414
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.4161/auto.1.2.1697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072289414
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.4161/auto.22110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072289631
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.4161/auto.27959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072289977
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.4161/auto.4600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072290239
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.4161/auto.6732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072290872
    273 rdf:type schema:CreativeWork
    274 https://www.grid.ac/institutes/grid.5522.0 schema:alternateName Jagiellonian University
    275 schema:name Departament of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland
    276 Department of Cell Biology and Imaging, Faculty of Biology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Kraków, Poland
    277 Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland
    278 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...