Novel histone deacetylase 8-selective inhibitor 1,3,4-oxadiazole-alanine hybrid induces apoptosis in breast cancer cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-11

AUTHORS

Vijaya Rao Pidugu, Nagendra Sastry Yarla, Anupam Bishayee, Arunasree M. Kalle, Alapati Krishna Satya

ABSTRACT

Identification of isoform-specific histone deacetylase inhibitors (HDACi) is a significant advantage to overcome the adverse side effects of pan-HDACi for the treatment of various diseases, including cancer. We have designed, and synthesized novel 1,3,4 oxadiazole with glycine/alanine hybrids as HDAC8-specific inhibitors and preliminary evaluation has indicated that 1,3,4 oxadiazole with alanine hybrid [(R)-2-amino-N-((5-phenyl-1,3,4-oxadiazol-2-yl)methyl)propanamide (10b)] to be a potent HDAC8 inhibitor. In the present study, the in vitro efficacy of the molecule in inhibiting the cancer cell proliferation and the underlying molecular mechanism was studied. 10b inhibited the growth of MDA-MB-231 and MCF7 breast cancer cells, with a lower IC50 of 230 and 1000 nM, respectively, compared to K562, COLO-205 and HepG2 cells and was not cytotoxic to normal breast epithelial cells, MCF10A. 10b was specific to HDAC8 and did not affect the expression of other class I HDACs. Further, a dose-dependent increase in H3K9 acetylation levels demonstrated the HDAC-inhibitory activity of 10b in MDA-MB-231 cells. Flow cytometric analysis indicated a dose-dependent increase and decrease in the percent apoptotic cells and mitochondrial membrane potential, respectively, when treated with 10b. Immunoblot analysis showed a modulation of Bax/Bcl2 ratio with a decrease in Bcl2 expression and no change in Bax expression. 10b treatment resulted in induction of p21 and inhibition of CDK1 proteins along with cytochrome c release from mitochondria, activation of caspases-3 and -9 and cleavage of poly ADP-ribose polymerase leading to apoptotic death of MDA-MB-231 and MCF7 cells. In conclusion, our results clearly demonstrated the efficacy of 10b as an anticancer agent against breast cancer. More... »

PAGES

1394-1403

References to SciGraph publications

  • 2006-11. Mutant p53 in MDA-MB-231 breast cancer cells is stabilized by elevated phospholipase D activity and contributes to survival signals generated by phospholipase D in ONCOGENE
  • 2007-08. Histone deacetylases and cancer in ONCOGENE
  • 2009-12. Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma in JOURNAL OF TRANSLATIONAL MEDICINE
  • 2007-02. Discovery and development of SAHA as an anticancer agent in ONCOGENE
  • 2007-06. Extraction, purification and analysis of histones in NATURE PROTOCOLS
  • 2014-09. Histone deacetylases and their inhibitors in cancer, neurological diseases and immune disorders in NATURE REVIEWS DRUG DISCOVERY
  • 2014-12. Histone deacetylase 8 is deregulated in urothelial cancer but not a target for efficient treatment in JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
  • 2015-02-19. Selective inhibition of HDAC8 decreases neuroblastoma growth in vitro and in vivo and enhances retinoic acid-mediated differentiation in CELL DEATH & DISEASE
  • 2013-01. Histone deacetylase inhibitors suppress mutant p53 transcription via histone deacetylase 8 in ONCOGENE
  • 2008-10. Bcl-2 family proteins and cancer in ONCOGENE
  • 2004-04. Purchase of Aton spotlights HDAC inhibitors in NATURE BIOTECHNOLOGY
  • 2013-02. Apaf-1- and Caspase-8-independent apoptosis in CELL DEATH & DIFFERENTIATION
  • 2015-12. Up regulation of Bax and down regulation of Bcl2 during 3-NC mediated apoptosis in human cancer cells in CANCER CELL INTERNATIONAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10495-017-1410-2

    DOI

    http://dx.doi.org/10.1007/s10495-017-1410-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1091323022

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28840369


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Oncology and Carcinogenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acetylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alanine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antineoplastic Agents", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Apoptosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CDC2 Protein Kinase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Proliferation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cyclin-Dependent Kinase Inhibitor p21", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelial Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Neoplastic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hep G2 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Histone Deacetylases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Histones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Inhibitory Concentration 50", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "K562 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "MCF-7 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Organ Specificity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oxadiazoles", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Poly(ADP-ribose) Polymerases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proto-Oncogene Proteins c-bcl-2", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Repressor Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Signal Transduction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "bcl-2-Associated X Protein", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Acharya Nagarjuna University", 
              "id": "https://www.grid.ac/institutes/grid.411114.0", 
              "name": [
                "GVK Biosciences Private Limited, IDA Mallapur, 500 076, Hyderabad, Telangana, India", 
                "Department of Biotechnology, Acharya Nagarjuna University, 522 510, Guntur, Andhra Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pidugu", 
            "givenName": "Vijaya Rao", 
            "id": "sg:person.014634101071.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014634101071.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Hyderabad", 
              "id": "https://www.grid.ac/institutes/grid.18048.35", 
              "name": [
                "Department of Biochemistry/Bioinformatics, Institute of Science, GITAM University, 530 045, Vishakhapatnam, Andhra Pradesh, India", 
                "Department of Animal Biology, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad, Telangana, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yarla", 
            "givenName": "Nagendra Sastry", 
            "id": "sg:person.01020313176.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01020313176.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Pharmaceutical Sciences, College of Pharmacy, Larkin University, 33169, Miami, FL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bishayee", 
            "givenName": "Anupam", 
            "id": "sg:person.01241647425.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241647425.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johns Hopkins University", 
              "id": "https://www.grid.ac/institutes/grid.21107.35", 
              "name": [
                "Department of Animal Biology, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad, Telangana, India", 
                "Department of Environmental Health Sciences, Laboratory of Human Environmental Epigenomes, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, 21205, Baltimore, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kalle", 
            "givenName": "Arunasree M.", 
            "id": "sg:person.0741327713.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741327713.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Acharya Nagarjuna University", 
              "id": "https://www.grid.ac/institutes/grid.411114.0", 
              "name": [
                "Department of Biotechnology, Acharya Nagarjuna University, 522 510, Guntur, Andhra Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Satya", 
            "givenName": "Alapati Krishna", 
            "id": "sg:person.010321351271.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010321351271.43"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1002/acn3.147", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000984525"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bmc.2016.09.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005269342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.185926.114", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005427498"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/onc.2008.307", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008538232", 
              "https://doi.org/10.1038/onc.2008.307"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2007.202", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009324955", 
              "https://doi.org/10.1038/nprot.2007.202"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cdd.2012.149", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010985034", 
              "https://doi.org/10.1038/cdd.2012.149"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/onc.2012.81", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012645003", 
              "https://doi.org/10.1038/onc.2012.81"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20070779", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013176616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20070779", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013176616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/excr.2000.5080", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014346356"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phymed.2009.12.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017131182"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.leukres.2007.11.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017781320"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1152/ajpcell.00102.2014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018014219"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1210204", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020984439", 
              "https://doi.org/10.1038/sj.onc.1210204"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbrc.2010.08.118", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021533073"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1210610", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023418889", 
              "https://doi.org/10.1038/sj.onc.1210610"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4155/fmc.12.3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023930744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1479-5876-7-110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028513159", 
              "https://doi.org/10.1186/1479-5876-7-110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tips.2015.04.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028748274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bmc.2010.03.080", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029231039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12935-015-0204-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029496861", 
              "https://doi.org/10.1186/s12935-015-0204-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12935-015-0204-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029496861", 
              "https://doi.org/10.1186/s12935-015-0204-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrd4360", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030225203", 
              "https://doi.org/10.1038/nrd4360"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2011.04.017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032956326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2015.08.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035621347"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0005-2728(03)00110-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036592380"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0005-2728(03)00110-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036592380"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1517/14728220903241658", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037766295"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.canlet.2008.10.047", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039114943"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m908988199", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040103939"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13046-014-0059-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040953304", 
              "https://doi.org/10.1186/s13046-014-0059-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cddis.2015.24", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041730794", 
              "https://doi.org/10.1038/cddis.2015.24"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bip.22135", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043284052"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/emboj.2009.395", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044867862"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0404-364", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045986162", 
              "https://doi.org/10.1038/nbt0404-364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0404-364", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045986162", 
              "https://doi.org/10.1038/nbt0404-364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1209735", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046439413", 
              "https://doi.org/10.1038/sj.onc.1209735"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1209735", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046439413", 
              "https://doi.org/10.1038/sj.onc.1209735"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1074-5521(02)00092-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049715681"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/bph.13018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051757178"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2008.03.025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052566369"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0404603101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053275756"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/cshperspect.a026104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060407187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1947601913484496", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064081171"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/0929867043365099", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069160299"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/156802609788085287", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069193788"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3892/or.2011.1236", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071531349"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083354389", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-11", 
        "datePublishedReg": "2017-11-01", 
        "description": "Identification of isoform-specific histone deacetylase inhibitors (HDACi) is a significant advantage to overcome the adverse side effects of pan-HDACi for the treatment of various diseases, including cancer. We have designed, and synthesized novel 1,3,4 oxadiazole with glycine/alanine hybrids as HDAC8-specific inhibitors and preliminary evaluation has indicated that 1,3,4 oxadiazole with alanine hybrid [(R)-2-amino-N-((5-phenyl-1,3,4-oxadiazol-2-yl)methyl)propanamide (10b)] to be a potent HDAC8 inhibitor. In the present study, the in vitro efficacy of the molecule in inhibiting the cancer cell proliferation and the underlying molecular mechanism was studied. 10b inhibited the growth of MDA-MB-231 and MCF7 breast cancer cells, with a lower IC50 of 230 and 1000\u00a0nM, respectively, compared to K562, COLO-205 and HepG2 cells and was not cytotoxic to normal breast epithelial cells, MCF10A. 10b was specific to HDAC8 and did not affect the expression of other class I HDACs. Further, a dose-dependent increase in H3K9 acetylation levels demonstrated the HDAC-inhibitory activity of 10b in MDA-MB-231 cells. Flow cytometric analysis indicated a dose-dependent increase and decrease in the percent apoptotic cells and mitochondrial membrane potential, respectively, when treated with 10b. Immunoblot analysis showed a modulation of Bax/Bcl2 ratio with a decrease in Bcl2 expression and no change in Bax expression. 10b treatment resulted in induction of p21 and inhibition of CDK1 proteins along with cytochrome c release from mitochondria, activation of caspases-3 and -9 and cleavage of poly ADP-ribose polymerase leading to apoptotic death of MDA-MB-231 and MCF7 cells. In conclusion, our results clearly demonstrated the efficacy of 10b as an anticancer agent against breast cancer.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s10495-017-1410-2", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1117236", 
            "issn": [
              "1360-8185", 
              "1573-675X"
            ], 
            "name": "Apoptosis", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "11", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "22"
          }
        ], 
        "name": "Novel histone deacetylase 8-selective inhibitor 1,3,4-oxadiazole-alanine hybrid induces apoptosis in breast cancer cells", 
        "pagination": "1394-1403", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "32009816f5a7e37ebb0366e4a816290d62f74d343f775ad700907ae1499ce781"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28840369"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9712129"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10495-017-1410-2"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1091323022"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10495-017-1410-2", 
          "https://app.dimensions.ai/details/publication/pub.1091323022"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T09:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99821_00000004.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs10495-017-1410-2"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10495-017-1410-2'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10495-017-1410-2'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10495-017-1410-2'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10495-017-1410-2'


     

    This table displays all metadata directly associated to this object as RDF triples.

    353 TRIPLES      21 PREDICATES      98 URIs      47 LITERALS      35 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10495-017-1410-2 schema:about N089ff34c9ace43669193d0a835dc4785
    2 N090f379fdb104dee9e5863e1fcc31cc9
    3 N1fd1e822dddd43e9accfa4f7c0addc55
    4 N21327b81c64d47be8edcf72ace1e3b71
    5 N2b180e7a11884d79b9cb12805c729861
    6 N366a95a5e16247e281abec51141061c2
    7 N3c1a9ec97c0a4395bde5f4110b31800a
    8 N3d02a0d4ac76448c864df5e59be855c7
    9 N4ab05d27102145f6ab7d62cb7aa31bd3
    10 N52e0278bceb34afe9a0c85419da4409b
    11 N557933c1d93f4d2ab6ce15f41b29357c
    12 N67ea5c753d804bc0af5edc066b91601a
    13 N70faf6a003f34505af3114d9a9600751
    14 Na4e1e045e5ff4497b5cd58128c51a720
    15 Na6a5870c6f624301af0c59e4b9d7ae7f
    16 Nacaacba3f9b040e6a4e72fa2326051ef
    17 Nadd48de087394ebcad5fb2d273ab92be
    18 Naf8ca9ebeb87469fa0dbb3a6ba9a12e0
    19 Nb6df7a8c27334ec7a6865510998be2ac
    20 Nbe1f57fa05c24eb88abf4f4a904fffdd
    21 Nbf7bc66ba4974945aac7436ceda92534
    22 Nc2fd6bdd3c5c4f838cda3b0dcf613873
    23 Nea09b235f6724090a008ba387e99aad8
    24 Nf00c8ae8bbba4aa9915338a2d1004005
    25 Nf3f4855bb21c4090b812372f5313136f
    26 Nf5d47b40965e454f942df27da3ce45ae
    27 anzsrc-for:11
    28 anzsrc-for:1112
    29 schema:author Nd9cce434fd0b4bb5b283e525e24451a2
    30 schema:citation sg:pub.10.1038/cdd.2012.149
    31 sg:pub.10.1038/cddis.2015.24
    32 sg:pub.10.1038/nbt0404-364
    33 sg:pub.10.1038/nprot.2007.202
    34 sg:pub.10.1038/nrd4360
    35 sg:pub.10.1038/onc.2008.307
    36 sg:pub.10.1038/onc.2012.81
    37 sg:pub.10.1038/sj.onc.1209735
    38 sg:pub.10.1038/sj.onc.1210204
    39 sg:pub.10.1038/sj.onc.1210610
    40 sg:pub.10.1186/1479-5876-7-110
    41 sg:pub.10.1186/s12935-015-0204-2
    42 sg:pub.10.1186/s13046-014-0059-8
    43 https://app.dimensions.ai/details/publication/pub.1083354389
    44 https://doi.org/10.1002/acn3.147
    45 https://doi.org/10.1002/bip.22135
    46 https://doi.org/10.1006/excr.2000.5080
    47 https://doi.org/10.1016/j.bbrc.2010.08.118
    48 https://doi.org/10.1016/j.bmc.2010.03.080
    49 https://doi.org/10.1016/j.bmc.2016.09.022
    50 https://doi.org/10.1016/j.canlet.2008.10.047
    51 https://doi.org/10.1016/j.cell.2008.03.025
    52 https://doi.org/10.1016/j.cell.2011.04.017
    53 https://doi.org/10.1016/j.leukres.2007.11.007
    54 https://doi.org/10.1016/j.phymed.2009.12.008
    55 https://doi.org/10.1016/j.stem.2015.08.004
    56 https://doi.org/10.1016/j.tips.2015.04.013
    57 https://doi.org/10.1016/s0005-2728(03)00110-5
    58 https://doi.org/10.1016/s1074-5521(02)00092-3
    59 https://doi.org/10.1038/emboj.2009.395
    60 https://doi.org/10.1042/bj20070779
    61 https://doi.org/10.1073/pnas.0404603101
    62 https://doi.org/10.1074/jbc.m908988199
    63 https://doi.org/10.1101/cshperspect.a026104
    64 https://doi.org/10.1101/gr.185926.114
    65 https://doi.org/10.1111/bph.13018
    66 https://doi.org/10.1152/ajpcell.00102.2014
    67 https://doi.org/10.1177/1947601913484496
    68 https://doi.org/10.1517/14728220903241658
    69 https://doi.org/10.2174/0929867043365099
    70 https://doi.org/10.2174/156802609788085287
    71 https://doi.org/10.3892/or.2011.1236
    72 https://doi.org/10.4155/fmc.12.3
    73 schema:datePublished 2017-11
    74 schema:datePublishedReg 2017-11-01
    75 schema:description Identification of isoform-specific histone deacetylase inhibitors (HDACi) is a significant advantage to overcome the adverse side effects of pan-HDACi for the treatment of various diseases, including cancer. We have designed, and synthesized novel 1,3,4 oxadiazole with glycine/alanine hybrids as HDAC8-specific inhibitors and preliminary evaluation has indicated that 1,3,4 oxadiazole with alanine hybrid [(R)-2-amino-N-((5-phenyl-1,3,4-oxadiazol-2-yl)methyl)propanamide (10b)] to be a potent HDAC8 inhibitor. In the present study, the in vitro efficacy of the molecule in inhibiting the cancer cell proliferation and the underlying molecular mechanism was studied. 10b inhibited the growth of MDA-MB-231 and MCF7 breast cancer cells, with a lower IC<sub>50</sub> of 230 and 1000 nM, respectively, compared to K562, COLO-205 and HepG2 cells and was not cytotoxic to normal breast epithelial cells, MCF10A. 10b was specific to HDAC8 and did not affect the expression of other class I HDACs. Further, a dose-dependent increase in H3K9 acetylation levels demonstrated the HDAC-inhibitory activity of 10b in MDA-MB-231 cells. Flow cytometric analysis indicated a dose-dependent increase and decrease in the percent apoptotic cells and mitochondrial membrane potential, respectively, when treated with 10b. Immunoblot analysis showed a modulation of Bax/Bcl2 ratio with a decrease in Bcl2 expression and no change in Bax expression. 10b treatment resulted in induction of p21 and inhibition of CDK1 proteins along with cytochrome c release from mitochondria, activation of caspases-3 and -9 and cleavage of poly ADP-ribose polymerase leading to apoptotic death of MDA-MB-231 and MCF7 cells. In conclusion, our results clearly demonstrated the efficacy of 10b as an anticancer agent against breast cancer.
    76 schema:genre research_article
    77 schema:inLanguage en
    78 schema:isAccessibleForFree false
    79 schema:isPartOf N275535c3fb0847c2bada560d5b134f0f
    80 N9b4145d8d94146d08a642061740c99ed
    81 sg:journal.1117236
    82 schema:name Novel histone deacetylase 8-selective inhibitor 1,3,4-oxadiazole-alanine hybrid induces apoptosis in breast cancer cells
    83 schema:pagination 1394-1403
    84 schema:productId N316a7435d3364c56958dd566748b76c7
    85 N4bf1d60c76aa4208a62f9dbfbfc83a02
    86 Nc716188dae3942158616091c71a5f596
    87 Ncf557a55937a48df859d4619aa18d835
    88 Nf4e9dd908e1547ddb2f0581b26e515cb
    89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091323022
    90 https://doi.org/10.1007/s10495-017-1410-2
    91 schema:sdDatePublished 2019-04-11T09:36
    92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    93 schema:sdPublisher N2d3d01e2b0544162b95633e2bdd4e7f1
    94 schema:url https://link.springer.com/10.1007%2Fs10495-017-1410-2
    95 sgo:license sg:explorer/license/
    96 sgo:sdDataset articles
    97 rdf:type schema:ScholarlyArticle
    98 N012b17531eca4f5cbb817d7918ea8237 schema:name Department of Pharmaceutical Sciences, College of Pharmacy, Larkin University, 33169, Miami, FL, USA
    99 rdf:type schema:Organization
    100 N089ff34c9ace43669193d0a835dc4785 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Cell Line
    102 rdf:type schema:DefinedTerm
    103 N090f379fdb104dee9e5863e1fcc31cc9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Hep G2 Cells
    105 rdf:type schema:DefinedTerm
    106 N1fd1e822dddd43e9accfa4f7c0addc55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Histones
    108 rdf:type schema:DefinedTerm
    109 N21327b81c64d47be8edcf72ace1e3b71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Proto-Oncogene Proteins c-bcl-2
    111 rdf:type schema:DefinedTerm
    112 N275535c3fb0847c2bada560d5b134f0f schema:issueNumber 11
    113 rdf:type schema:PublicationIssue
    114 N2b180e7a11884d79b9cb12805c729861 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Gene Expression Regulation, Neoplastic
    116 rdf:type schema:DefinedTerm
    117 N2d3d01e2b0544162b95633e2bdd4e7f1 schema:name Springer Nature - SN SciGraph project
    118 rdf:type schema:Organization
    119 N316a7435d3364c56958dd566748b76c7 schema:name dimensions_id
    120 schema:value pub.1091323022
    121 rdf:type schema:PropertyValue
    122 N366a95a5e16247e281abec51141061c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Female
    124 rdf:type schema:DefinedTerm
    125 N3c1a9ec97c0a4395bde5f4110b31800a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Repressor Proteins
    127 rdf:type schema:DefinedTerm
    128 N3d02a0d4ac76448c864df5e59be855c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Epithelial Cells
    130 rdf:type schema:DefinedTerm
    131 N4ab05d27102145f6ab7d62cb7aa31bd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Organ Specificity
    133 rdf:type schema:DefinedTerm
    134 N4bf1d60c76aa4208a62f9dbfbfc83a02 schema:name nlm_unique_id
    135 schema:value 9712129
    136 rdf:type schema:PropertyValue
    137 N52e0278bceb34afe9a0c85419da4409b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Acetylation
    139 rdf:type schema:DefinedTerm
    140 N557933c1d93f4d2ab6ce15f41b29357c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Cell Proliferation
    142 rdf:type schema:DefinedTerm
    143 N67ea5c753d804bc0af5edc066b91601a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Oxadiazoles
    145 rdf:type schema:DefinedTerm
    146 N70faf6a003f34505af3114d9a9600751 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Cyclin-Dependent Kinase Inhibitor p21
    148 rdf:type schema:DefinedTerm
    149 N9b4145d8d94146d08a642061740c99ed schema:volumeNumber 22
    150 rdf:type schema:PublicationVolume
    151 Na4e1e045e5ff4497b5cd58128c51a720 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name bcl-2-Associated X Protein
    153 rdf:type schema:DefinedTerm
    154 Na6a5870c6f624301af0c59e4b9d7ae7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Humans
    156 rdf:type schema:DefinedTerm
    157 Nacaacba3f9b040e6a4e72fa2326051ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name MCF-7 Cells
    159 rdf:type schema:DefinedTerm
    160 Nadd48de087394ebcad5fb2d273ab92be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Antineoplastic Agents
    162 rdf:type schema:DefinedTerm
    163 Naf8ca9ebeb87469fa0dbb3a6ba9a12e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Histone Deacetylases
    165 rdf:type schema:DefinedTerm
    166 Nb5d5cac7f1d74693ad202a77744607cb rdf:first sg:person.01241647425.29
    167 rdf:rest Nc04b5032a0b84084b9a04a209ed54566
    168 Nb6df7a8c27334ec7a6865510998be2ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Alanine
    170 rdf:type schema:DefinedTerm
    171 Nbe1f57fa05c24eb88abf4f4a904fffdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name CDC2 Protein Kinase
    173 rdf:type schema:DefinedTerm
    174 Nbf6836a3b35e4caa8abb9e78dfd88c20 rdf:first sg:person.01020313176.85
    175 rdf:rest Nb5d5cac7f1d74693ad202a77744607cb
    176 Nbf7bc66ba4974945aac7436ceda92534 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name K562 Cells
    178 rdf:type schema:DefinedTerm
    179 Nc04b5032a0b84084b9a04a209ed54566 rdf:first sg:person.0741327713.02
    180 rdf:rest Nd8e2311df5b54da884ea1f16081a64fe
    181 Nc2fd6bdd3c5c4f838cda3b0dcf613873 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Signal Transduction
    183 rdf:type schema:DefinedTerm
    184 Nc716188dae3942158616091c71a5f596 schema:name readcube_id
    185 schema:value 32009816f5a7e37ebb0366e4a816290d62f74d343f775ad700907ae1499ce781
    186 rdf:type schema:PropertyValue
    187 Ncf557a55937a48df859d4619aa18d835 schema:name pubmed_id
    188 schema:value 28840369
    189 rdf:type schema:PropertyValue
    190 Nd8e2311df5b54da884ea1f16081a64fe rdf:first sg:person.010321351271.43
    191 rdf:rest rdf:nil
    192 Nd9cce434fd0b4bb5b283e525e24451a2 rdf:first sg:person.014634101071.28
    193 rdf:rest Nbf6836a3b35e4caa8abb9e78dfd88c20
    194 Nea09b235f6724090a008ba387e99aad8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    195 schema:name Apoptosis
    196 rdf:type schema:DefinedTerm
    197 Nf00c8ae8bbba4aa9915338a2d1004005 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    198 schema:name Poly(ADP-ribose) Polymerases
    199 rdf:type schema:DefinedTerm
    200 Nf3f4855bb21c4090b812372f5313136f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    201 schema:name Cell Line, Tumor
    202 rdf:type schema:DefinedTerm
    203 Nf4e9dd908e1547ddb2f0581b26e515cb schema:name doi
    204 schema:value 10.1007/s10495-017-1410-2
    205 rdf:type schema:PropertyValue
    206 Nf5d47b40965e454f942df27da3ce45ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    207 schema:name Inhibitory Concentration 50
    208 rdf:type schema:DefinedTerm
    209 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    210 schema:name Medical and Health Sciences
    211 rdf:type schema:DefinedTerm
    212 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
    213 schema:name Oncology and Carcinogenesis
    214 rdf:type schema:DefinedTerm
    215 sg:journal.1117236 schema:issn 1360-8185
    216 1573-675X
    217 schema:name Apoptosis
    218 rdf:type schema:Periodical
    219 sg:person.01020313176.85 schema:affiliation https://www.grid.ac/institutes/grid.18048.35
    220 schema:familyName Yarla
    221 schema:givenName Nagendra Sastry
    222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01020313176.85
    223 rdf:type schema:Person
    224 sg:person.010321351271.43 schema:affiliation https://www.grid.ac/institutes/grid.411114.0
    225 schema:familyName Satya
    226 schema:givenName Alapati Krishna
    227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010321351271.43
    228 rdf:type schema:Person
    229 sg:person.01241647425.29 schema:affiliation N012b17531eca4f5cbb817d7918ea8237
    230 schema:familyName Bishayee
    231 schema:givenName Anupam
    232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241647425.29
    233 rdf:type schema:Person
    234 sg:person.014634101071.28 schema:affiliation https://www.grid.ac/institutes/grid.411114.0
    235 schema:familyName Pidugu
    236 schema:givenName Vijaya Rao
    237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014634101071.28
    238 rdf:type schema:Person
    239 sg:person.0741327713.02 schema:affiliation https://www.grid.ac/institutes/grid.21107.35
    240 schema:familyName Kalle
    241 schema:givenName Arunasree M.
    242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741327713.02
    243 rdf:type schema:Person
    244 sg:pub.10.1038/cdd.2012.149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010985034
    245 https://doi.org/10.1038/cdd.2012.149
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1038/cddis.2015.24 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041730794
    248 https://doi.org/10.1038/cddis.2015.24
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1038/nbt0404-364 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045986162
    251 https://doi.org/10.1038/nbt0404-364
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1038/nprot.2007.202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009324955
    254 https://doi.org/10.1038/nprot.2007.202
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1038/nrd4360 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030225203
    257 https://doi.org/10.1038/nrd4360
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1038/onc.2008.307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008538232
    260 https://doi.org/10.1038/onc.2008.307
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1038/onc.2012.81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012645003
    263 https://doi.org/10.1038/onc.2012.81
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1038/sj.onc.1209735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046439413
    266 https://doi.org/10.1038/sj.onc.1209735
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1038/sj.onc.1210204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020984439
    269 https://doi.org/10.1038/sj.onc.1210204
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/sj.onc.1210610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023418889
    272 https://doi.org/10.1038/sj.onc.1210610
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1186/1479-5876-7-110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028513159
    275 https://doi.org/10.1186/1479-5876-7-110
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1186/s12935-015-0204-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029496861
    278 https://doi.org/10.1186/s12935-015-0204-2
    279 rdf:type schema:CreativeWork
    280 sg:pub.10.1186/s13046-014-0059-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040953304
    281 https://doi.org/10.1186/s13046-014-0059-8
    282 rdf:type schema:CreativeWork
    283 https://app.dimensions.ai/details/publication/pub.1083354389 schema:CreativeWork
    284 https://doi.org/10.1002/acn3.147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000984525
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1002/bip.22135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043284052
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1006/excr.2000.5080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014346356
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1016/j.bbrc.2010.08.118 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021533073
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1016/j.bmc.2010.03.080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029231039
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1016/j.bmc.2016.09.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005269342
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1016/j.canlet.2008.10.047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039114943
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1016/j.cell.2008.03.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052566369
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1016/j.cell.2011.04.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032956326
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1016/j.leukres.2007.11.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017781320
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1016/j.phymed.2009.12.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017131182
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1016/j.stem.2015.08.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035621347
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1016/j.tips.2015.04.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028748274
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1016/s0005-2728(03)00110-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036592380
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1016/s1074-5521(02)00092-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049715681
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1038/emboj.2009.395 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044867862
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1042/bj20070779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013176616
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1073/pnas.0404603101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053275756
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.1074/jbc.m908988199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040103939
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.1101/cshperspect.a026104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060407187
    323 rdf:type schema:CreativeWork
    324 https://doi.org/10.1101/gr.185926.114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005427498
    325 rdf:type schema:CreativeWork
    326 https://doi.org/10.1111/bph.13018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051757178
    327 rdf:type schema:CreativeWork
    328 https://doi.org/10.1152/ajpcell.00102.2014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018014219
    329 rdf:type schema:CreativeWork
    330 https://doi.org/10.1177/1947601913484496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064081171
    331 rdf:type schema:CreativeWork
    332 https://doi.org/10.1517/14728220903241658 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037766295
    333 rdf:type schema:CreativeWork
    334 https://doi.org/10.2174/0929867043365099 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069160299
    335 rdf:type schema:CreativeWork
    336 https://doi.org/10.2174/156802609788085287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069193788
    337 rdf:type schema:CreativeWork
    338 https://doi.org/10.3892/or.2011.1236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071531349
    339 rdf:type schema:CreativeWork
    340 https://doi.org/10.4155/fmc.12.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023930744
    341 rdf:type schema:CreativeWork
    342 https://www.grid.ac/institutes/grid.18048.35 schema:alternateName University of Hyderabad
    343 schema:name Department of Animal Biology, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad, Telangana, India
    344 Department of Biochemistry/Bioinformatics, Institute of Science, GITAM University, 530 045, Vishakhapatnam, Andhra Pradesh, India
    345 rdf:type schema:Organization
    346 https://www.grid.ac/institutes/grid.21107.35 schema:alternateName Johns Hopkins University
    347 schema:name Department of Animal Biology, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad, Telangana, India
    348 Department of Environmental Health Sciences, Laboratory of Human Environmental Epigenomes, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, 21205, Baltimore, MD, USA
    349 rdf:type schema:Organization
    350 https://www.grid.ac/institutes/grid.411114.0 schema:alternateName Acharya Nagarjuna University
    351 schema:name Department of Biotechnology, Acharya Nagarjuna University, 522 510, Guntur, Andhra Pradesh, India
    352 GVK Biosciences Private Limited, IDA Mallapur, 500 076, Hyderabad, Telangana, India
    353 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...