Amsacrine-induced apoptosis of human leukemia U937 cells is mediated by the inhibition of AKT- and ERK-induced stabilization of MCL1 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-10-19

AUTHORS

Yuan-Chin Lee, Ying-Jung Chen, Chia-Hui Huang, Long-Sen Chang

ABSTRACT

Previous studies have attributed the anticancer activity of amsacrine to its inhibitory effect on topoisomerase II. However, 9-aminoacridine derivatives, which have the same structural scaffold as amsacrine, induce cancer cell apoptosis by altering the expression of BCL2 family proteins. Therefore, in the present study, we assessed whether BCL2 family proteins mediated the cytotoxic effects of amsacrine on human leukemia U937 cells. Amsacrine-induced apoptosis of U937 cells was characterized by caspase-9 and caspase-3 activation, increased intracellular Ca2+ concentration, mitochondrial depolarization, and MCL1 down-regulation. Amsacrine induced MCL1 down-regulation by decreasing its stability. Further, amsacrine-treated U937 cells showed AKT degradation and Ca2+-mediated ERK inactivation. Blockade of ERK-mediated phosphorylation of MCL1 inhibited the effect of Pin1 on the stabilization of MCL1, and AKT degradation promoted GSK3β-mediated degradation of MCL1. Restoration of ERK phosphorylation and AKT expression abrogated amsacrine-induced MCL1 down-regulation. Moreover, MCL1 over-expression inhibited amsacrine-induced depolarization of mitochondria membrane and increased the viability of amsacrine-treated cells. Taken together, our data indicate that amsacrine abolishes ERK- and Pin1-mediated stabilization of MCL1 and promotes GSK3β-mediated degradation of MCL1, leading to activate mitochondria-mediated apoptosis pathway in U937 cells. More... »

PAGES

406-420

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10495-016-1307-5

DOI

http://dx.doi.org/10.1007/s10495-016-1307-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023771500

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27757735


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amsacrine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antineoplastic Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Etoposide", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Extracellular Signal-Regulated MAP Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glycogen Synthase Kinase 3 beta", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "MAP Kinase Kinase 1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "MAP Kinase Signaling System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myeloid Cell Leukemia Sequence 1 Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NIMA-Interacting Peptidylprolyl Isomerase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteasome Endopeptidase Complex", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Processing, Post-Translational", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Stability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proto-Oncogene Proteins c-akt", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reactive Oxygen Species", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "U937 Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Yuan-Chin", 
        "id": "sg:person.01043111067.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043111067.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Ying-Jung", 
        "id": "sg:person.0726732663.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726732663.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412036.2", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Chia-Hui", 
        "id": "sg:person.01152152662.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152152662.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biotechnology, Kaohsiung Medical University, 807, Kaohsiung, Taiwan", 
          "id": "http://www.grid.ac/institutes/grid.412019.f", 
          "name": [
            "Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan", 
            "Department of Biotechnology, Kaohsiung Medical University, 807, Kaohsiung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Long-Sen", 
        "id": "sg:person.0770353614.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770353614.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sj.onc.1207692", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040174526", 
          "https://doi.org/10.1038/sj.onc.1207692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4401978", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032305419", 
          "https://doi.org/10.1038/sj.cdd.4401978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1009616228304", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051832738", 
          "https://doi.org/10.1023/a:1009616228304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/onc.2008.460", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034135194", 
          "https://doi.org/10.1038/onc.2008.460"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1478-811x-9-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051680347", 
          "https://doi.org/10.1186/1478-811x-9-13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm2308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038545301", 
          "https://doi.org/10.1038/nrm2308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm3722", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001448882", 
          "https://doi.org/10.1038/nrm3722"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-10-19", 
    "datePublishedReg": "2016-10-19", 
    "description": "Previous studies have attributed the anticancer activity of amsacrine to its inhibitory effect on topoisomerase II. However, 9-aminoacridine derivatives, which have the same structural scaffold as amsacrine, induce cancer cell apoptosis by altering the expression of BCL2 family proteins. Therefore, in the present study, we assessed whether BCL2 family proteins mediated the cytotoxic effects of amsacrine on human leukemia U937 cells. Amsacrine-induced apoptosis of U937 cells was characterized by caspase-9 and caspase-3 activation, increased intracellular Ca2+ concentration, mitochondrial depolarization, and MCL1 down-regulation. Amsacrine induced MCL1 down-regulation by decreasing its stability. Further, amsacrine-treated U937 cells showed AKT degradation and Ca2+-mediated ERK inactivation. Blockade of ERK-mediated phosphorylation of MCL1 inhibited the effect of Pin1 on the stabilization of MCL1, and AKT degradation promoted GSK3\u03b2-mediated degradation of MCL1. Restoration of ERK phosphorylation and AKT expression abrogated amsacrine-induced MCL1 down-regulation. Moreover, MCL1 over-expression inhibited amsacrine-induced depolarization of mitochondria membrane and increased the viability of amsacrine-treated cells. Taken together, our data indicate that amsacrine abolishes ERK- and Pin1-mediated stabilization of MCL1 and promotes GSK3\u03b2-mediated degradation of MCL1, leading to activate mitochondria-mediated apoptosis pathway in U937 cells.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s10495-016-1307-5", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1117236", 
        "issn": [
          "1360-8185", 
          "1573-675X"
        ], 
        "name": "Apoptosis", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "22"
      }
    ], 
    "keywords": [
      "GSK3\u03b2-mediated degradation", 
      "U937 cells", 
      "BCL2 family proteins", 
      "blockade of ERK", 
      "cancer cell apoptosis", 
      "leukemia U937 cells", 
      "human leukemia U937 cells", 
      "effect of Pin1", 
      "inhibition of Akt", 
      "intracellular Ca2", 
      "Akt degradation", 
      "caspase-3 activation", 
      "Akt expression", 
      "inhibitory effect", 
      "cell apoptosis", 
      "cytotoxic effects", 
      "ERK phosphorylation", 
      "amsacrine", 
      "MCL1", 
      "ERK", 
      "apoptosis pathway", 
      "apoptosis", 
      "anticancer activity", 
      "mitochondrial depolarization", 
      "caspase-9", 
      "present study", 
      "ERK inactivation", 
      "topoisomerase II", 
      "cells", 
      "depolarization", 
      "family proteins", 
      "expression", 
      "Ca2", 
      "previous studies", 
      "mitochondria membrane", 
      "blockade", 
      "same structural scaffold", 
      "phosphorylation", 
      "Akt", 
      "protein", 
      "effect", 
      "inhibition", 
      "study", 
      "activation", 
      "regulation", 
      "Pin1", 
      "pathway", 
      "inactivation", 
      "structural scaffold", 
      "viability", 
      "activity", 
      "restoration", 
      "stabilization", 
      "concentration", 
      "membrane", 
      "data", 
      "degradation", 
      "derivatives", 
      "scaffolds", 
      "stability"
    ], 
    "name": "Amsacrine-induced apoptosis of human leukemia U937 cells is mediated by the inhibition of AKT- and ERK-induced stabilization of MCL1", 
    "pagination": "406-420", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023771500"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10495-016-1307-5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27757735"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10495-016-1307-5", 
      "https://app.dimensions.ai/details/publication/pub.1023771500"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:41", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_687.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s10495-016-1307-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10495-016-1307-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10495-016-1307-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10495-016-1307-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10495-016-1307-5'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      113 URIs      98 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10495-016-1307-5 schema:about N1e2edde38b8544949021643ac4764fa6
2 N29c95c4b63f546edaaf11f6ab9df8423
3 N2b1ec4c6c07e4c2a850adc96f69a5b84
4 N2b9d6dac45d84cdcbc5d63598afc7cdd
5 N35e02f05b0824f3098b94464704e9a94
6 N3a1c28ce876647ba9fecd2a212bac477
7 N3fc2eb42bb5647edadc4f0d316c51dd7
8 N46483b979ef14cdab9f44c825ff5e38e
9 N47365241b8b847698ac9750134f8cf5c
10 N6ba559eb47e64b7fa24841e8b27ed7c2
11 N6f57e6aea7c94fd0a6a0e3c8feb7ef9d
12 N72529d14c24243c48b31cdbceb548244
13 N7bb75503f71a4388ae8fa1a70cff2917
14 N831a5413f8494c4d832087fc972615dc
15 N9aa1aee082f64b11b807e77e52179cdc
16 Na7ddbd77f9164c1baa8503e5148a8bb6
17 Nbf1e150ec90944a09dac8484d17a4ed7
18 Nce4671c5c7884941a3fe68883aacd4b2
19 Ncf4e8ea209db467298fd36f9d4c81900
20 Ne4614481637d4e6c9e51abb7499ac6a1
21 Nf439febb9510487db5569c9817067961
22 anzsrc-for:06
23 anzsrc-for:0601
24 schema:author N014f02f5dede409f8e9ba2b6a2bbcd25
25 schema:citation sg:pub.10.1023/a:1009616228304
26 sg:pub.10.1038/nrm2308
27 sg:pub.10.1038/nrm3722
28 sg:pub.10.1038/onc.2008.460
29 sg:pub.10.1038/sj.cdd.4401978
30 sg:pub.10.1038/sj.onc.1207692
31 sg:pub.10.1186/1478-811x-9-13
32 schema:datePublished 2016-10-19
33 schema:datePublishedReg 2016-10-19
34 schema:description Previous studies have attributed the anticancer activity of amsacrine to its inhibitory effect on topoisomerase II. However, 9-aminoacridine derivatives, which have the same structural scaffold as amsacrine, induce cancer cell apoptosis by altering the expression of BCL2 family proteins. Therefore, in the present study, we assessed whether BCL2 family proteins mediated the cytotoxic effects of amsacrine on human leukemia U937 cells. Amsacrine-induced apoptosis of U937 cells was characterized by caspase-9 and caspase-3 activation, increased intracellular Ca2+ concentration, mitochondrial depolarization, and MCL1 down-regulation. Amsacrine induced MCL1 down-regulation by decreasing its stability. Further, amsacrine-treated U937 cells showed AKT degradation and Ca2+-mediated ERK inactivation. Blockade of ERK-mediated phosphorylation of MCL1 inhibited the effect of Pin1 on the stabilization of MCL1, and AKT degradation promoted GSK3β-mediated degradation of MCL1. Restoration of ERK phosphorylation and AKT expression abrogated amsacrine-induced MCL1 down-regulation. Moreover, MCL1 over-expression inhibited amsacrine-induced depolarization of mitochondria membrane and increased the viability of amsacrine-treated cells. Taken together, our data indicate that amsacrine abolishes ERK- and Pin1-mediated stabilization of MCL1 and promotes GSK3β-mediated degradation of MCL1, leading to activate mitochondria-mediated apoptosis pathway in U937 cells.
35 schema:genre article
36 schema:isAccessibleForFree false
37 schema:isPartOf N07670a64b95d4ed1aa82e6183d135e4e
38 N7ce0567697e547fc978ec29f32abed67
39 sg:journal.1117236
40 schema:keywords Akt
41 Akt degradation
42 Akt expression
43 BCL2 family proteins
44 Ca2
45 ERK
46 ERK inactivation
47 ERK phosphorylation
48 GSK3β-mediated degradation
49 MCL1
50 Pin1
51 U937 cells
52 activation
53 activity
54 amsacrine
55 anticancer activity
56 apoptosis
57 apoptosis pathway
58 blockade
59 blockade of ERK
60 cancer cell apoptosis
61 caspase-3 activation
62 caspase-9
63 cell apoptosis
64 cells
65 concentration
66 cytotoxic effects
67 data
68 degradation
69 depolarization
70 derivatives
71 effect
72 effect of Pin1
73 expression
74 family proteins
75 human leukemia U937 cells
76 inactivation
77 inhibition
78 inhibition of Akt
79 inhibitory effect
80 intracellular Ca2
81 leukemia U937 cells
82 membrane
83 mitochondria membrane
84 mitochondrial depolarization
85 pathway
86 phosphorylation
87 present study
88 previous studies
89 protein
90 regulation
91 restoration
92 same structural scaffold
93 scaffolds
94 stability
95 stabilization
96 structural scaffold
97 study
98 topoisomerase II
99 viability
100 schema:name Amsacrine-induced apoptosis of human leukemia U937 cells is mediated by the inhibition of AKT- and ERK-induced stabilization of MCL1
101 schema:pagination 406-420
102 schema:productId N5a1b98c9508d48fcb0c8f45ad3373b22
103 N647987cdb9d14e9ebae743987db3152e
104 N75424c4d80d7454f8690afae847a3aa0
105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023771500
106 https://doi.org/10.1007/s10495-016-1307-5
107 schema:sdDatePublished 2022-10-01T06:41
108 schema:sdLicense https://scigraph.springernature.com/explorer/license/
109 schema:sdPublisher Neb922c604a6b4d7f91382bc3d9f46f74
110 schema:url https://doi.org/10.1007/s10495-016-1307-5
111 sgo:license sg:explorer/license/
112 sgo:sdDataset articles
113 rdf:type schema:ScholarlyArticle
114 N014f02f5dede409f8e9ba2b6a2bbcd25 rdf:first sg:person.01043111067.02
115 rdf:rest Ne5ef1270bf534bc3827dca8a8736e5d1
116 N07670a64b95d4ed1aa82e6183d135e4e schema:volumeNumber 22
117 rdf:type schema:PublicationVolume
118 N1e2edde38b8544949021643ac4764fa6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Reactive Oxygen Species
120 rdf:type schema:DefinedTerm
121 N29c95c4b63f546edaaf11f6ab9df8423 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Extracellular Signal-Regulated MAP Kinases
123 rdf:type schema:DefinedTerm
124 N2b1ec4c6c07e4c2a850adc96f69a5b84 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name NIMA-Interacting Peptidylprolyl Isomerase
126 rdf:type schema:DefinedTerm
127 N2b9d6dac45d84cdcbc5d63598afc7cdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Glycogen Synthase Kinase 3 beta
129 rdf:type schema:DefinedTerm
130 N35e02f05b0824f3098b94464704e9a94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Etoposide
132 rdf:type schema:DefinedTerm
133 N3a1c28ce876647ba9fecd2a212bac477 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Proteasome Endopeptidase Complex
135 rdf:type schema:DefinedTerm
136 N3fc2eb42bb5647edadc4f0d316c51dd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Recombinant Proteins
138 rdf:type schema:DefinedTerm
139 N46483b979ef14cdab9f44c825ff5e38e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Amsacrine
141 rdf:type schema:DefinedTerm
142 N47365241b8b847698ac9750134f8cf5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name U937 Cells
144 rdf:type schema:DefinedTerm
145 N5a1b98c9508d48fcb0c8f45ad3373b22 schema:name doi
146 schema:value 10.1007/s10495-016-1307-5
147 rdf:type schema:PropertyValue
148 N647987cdb9d14e9ebae743987db3152e schema:name dimensions_id
149 schema:value pub.1023771500
150 rdf:type schema:PropertyValue
151 N6ba559eb47e64b7fa24841e8b27ed7c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Protein Processing, Post-Translational
153 rdf:type schema:DefinedTerm
154 N6f57e6aea7c94fd0a6a0e3c8feb7ef9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Proto-Oncogene Proteins c-akt
156 rdf:type schema:DefinedTerm
157 N72529d14c24243c48b31cdbceb548244 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Humans
159 rdf:type schema:DefinedTerm
160 N75424c4d80d7454f8690afae847a3aa0 schema:name pubmed_id
161 schema:value 27757735
162 rdf:type schema:PropertyValue
163 N754b8abd7fb34b0b96f7bd8da90021a3 rdf:first sg:person.0770353614.14
164 rdf:rest rdf:nil
165 N7bb75503f71a4388ae8fa1a70cff2917 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name MAP Kinase Kinase 1
167 rdf:type schema:DefinedTerm
168 N7ce0567697e547fc978ec29f32abed67 schema:issueNumber 3
169 rdf:type schema:PublicationIssue
170 N831a5413f8494c4d832087fc972615dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Apoptosis
172 rdf:type schema:DefinedTerm
173 N9aa1aee082f64b11b807e77e52179cdc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Signal Transduction
175 rdf:type schema:DefinedTerm
176 Na7ddbd77f9164c1baa8503e5148a8bb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Antineoplastic Agents
178 rdf:type schema:DefinedTerm
179 Nb69ab4e98d1343afa0d0b21bf9f959b9 rdf:first sg:person.01152152662.22
180 rdf:rest N754b8abd7fb34b0b96f7bd8da90021a3
181 Nbf1e150ec90944a09dac8484d17a4ed7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Protein Stability
183 rdf:type schema:DefinedTerm
184 Nce4671c5c7884941a3fe68883aacd4b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Phosphorylation
186 rdf:type schema:DefinedTerm
187 Ncf4e8ea209db467298fd36f9d4c81900 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name MAP Kinase Signaling System
189 rdf:type schema:DefinedTerm
190 Ne4614481637d4e6c9e51abb7499ac6a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Calcium
192 rdf:type schema:DefinedTerm
193 Ne5ef1270bf534bc3827dca8a8736e5d1 rdf:first sg:person.0726732663.35
194 rdf:rest Nb69ab4e98d1343afa0d0b21bf9f959b9
195 Neb922c604a6b4d7f91382bc3d9f46f74 schema:name Springer Nature - SN SciGraph project
196 rdf:type schema:Organization
197 Nf439febb9510487db5569c9817067961 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Myeloid Cell Leukemia Sequence 1 Protein
199 rdf:type schema:DefinedTerm
200 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
201 schema:name Biological Sciences
202 rdf:type schema:DefinedTerm
203 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
204 schema:name Biochemistry and Cell Biology
205 rdf:type schema:DefinedTerm
206 sg:journal.1117236 schema:issn 1360-8185
207 1573-675X
208 schema:name Apoptosis
209 schema:publisher Springer Nature
210 rdf:type schema:Periodical
211 sg:person.01043111067.02 schema:affiliation grid-institutes:grid.412036.2
212 schema:familyName Lee
213 schema:givenName Yuan-Chin
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043111067.02
215 rdf:type schema:Person
216 sg:person.01152152662.22 schema:affiliation grid-institutes:grid.412036.2
217 schema:familyName Huang
218 schema:givenName Chia-Hui
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152152662.22
220 rdf:type schema:Person
221 sg:person.0726732663.35 schema:affiliation grid-institutes:grid.412036.2
222 schema:familyName Chen
223 schema:givenName Ying-Jung
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726732663.35
225 rdf:type schema:Person
226 sg:person.0770353614.14 schema:affiliation grid-institutes:grid.412019.f
227 schema:familyName Chang
228 schema:givenName Long-Sen
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770353614.14
230 rdf:type schema:Person
231 sg:pub.10.1023/a:1009616228304 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051832738
232 https://doi.org/10.1023/a:1009616228304
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nrm2308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038545301
235 https://doi.org/10.1038/nrm2308
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/nrm3722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001448882
238 https://doi.org/10.1038/nrm3722
239 rdf:type schema:CreativeWork
240 sg:pub.10.1038/onc.2008.460 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034135194
241 https://doi.org/10.1038/onc.2008.460
242 rdf:type schema:CreativeWork
243 sg:pub.10.1038/sj.cdd.4401978 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032305419
244 https://doi.org/10.1038/sj.cdd.4401978
245 rdf:type schema:CreativeWork
246 sg:pub.10.1038/sj.onc.1207692 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040174526
247 https://doi.org/10.1038/sj.onc.1207692
248 rdf:type schema:CreativeWork
249 sg:pub.10.1186/1478-811x-9-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051680347
250 https://doi.org/10.1186/1478-811x-9-13
251 rdf:type schema:CreativeWork
252 grid-institutes:grid.412019.f schema:alternateName Department of Biotechnology, Kaohsiung Medical University, 807, Kaohsiung, Taiwan
253 schema:name Department of Biotechnology, Kaohsiung Medical University, 807, Kaohsiung, Taiwan
254 Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan
255 rdf:type schema:Organization
256 grid-institutes:grid.412036.2 schema:alternateName Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan
257 schema:name Institute of Biomedical Sciences, National Sun Yat-Sen University, 804, Kaohsiung, Taiwan
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...