Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR–RFLP of kdr ... View Full Text


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Article Info

DATE

2019-02

AUTHORS

Jitka Stara, Stano Pekar, Marta Nesvorna, Tomas Erban, Hana Vinsova, Jan Kopecky, Ivo Doskocil, Martin Kamler, Jan Hubert

ABSTRACT

Varroa destructor is the major cause of honey bee (Apis mellifera) colony losses. Mite control is limited to several miticides. The overuse of tau-fluvalinate has resulted in resistance via a knockdown resistance (kdr) mutation in the sodium channel gene NaVChs (L925V/I/M). In this study, we used the discriminating concentration of tau-fluvalinate (0.25 µg/mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (NaVCh), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites. Resistance values corresponding to the densitometry of the resistant allele were related to mite survival. In the vial test, the survival of the control group was significantly higher (70.4%) than that of the tau-fluvalinate-treated group (34.3%). Mite survival in the vial test was significantly correlated with the mean proportion of resistance values. Individuals that died after tau-fluvalinate application exhibited an average resistance value of 0.0783, whereas individuals that survived exhibited an average resistance of 0.400. The concentration of tau-fluvalinate in the vials was checked using high performance liquid chromatography under different temperatures and exposure times, and indicates that the stability of tau-fluvalinate stored in the refrigerator (4 ± 1 °C) is at least 14 days. PCR-RFLP of the NaVCh gene fragment verified that the vial test is a suitable, rapid, and cost-effective method for the identification of tau-fluvalinate resistance based on kdr mutation in V. destructor in apiaries. More... »

PAGES

161-171

References to SciGraph publications

  • 2018-06. New PCR–RFLP diagnostics methodology for detecting Varroa destructor resistant to synthetic pyrethroids in JOURNAL OF PEST SCIENCE
  • 1998. Monitoring Varroa jacobsoni resistance to pyrethroids in western Europe in APIDOLOGIE
  • 2002-07. Decline in the proportion of mites resistant to fluvalinate in a population of Varroa destructor not treated with pyrethroids in APIDOLOGIE
  • 2016-05. Comparison of tau-fluvalinate, acrinathrin, and amitraz effects on susceptible and resistant populations of Varroa destructor in a vial test in EXPERIMENTAL AND APPLIED ACAROLOGY
  • 1995. The resistance of Varroa jacobsoni Oud to pyrethroids: a laboratory assay in APIDOLOGIE
  • 2000-05. Control of Varroa jacobsoni Oud. resistant to fluvalinate and amitraz using coumaphos in APIDOLOGIE
  • 2018-06. A single mutation is driving resistance to pyrethroids in European populations of the parasitic mite, Varroa destructor in JOURNAL OF PEST SCIENCE
  • 1999. Detection of resistance in US Varroa jacobsoni Oud. (Mesostigmata: Varroidae) to the acaricide fluvalinate in APIDOLOGIE
  • 1997. Fluvalinate resistance of Varroa jacobsoni Oudemans (Acari: Varroidae) in Mediterranean apiaries of France in APIDOLOGIE
  • 2011-05. Predicting pesticide fate in the hive (part 1): experimentally determined τ-fluvalinate residues in bees, honey and wax in APIDOLOGIE
  • 1999. The resistance of Varroa jacobsoni Oud. to acaricides in APIDOLOGIE
  • 1996. Determination of the LC50 in the mite Varroa jacobsoni of the active substances in Perizin® and Cekafix® in APIDOLOGIE
  • 2015-11. A new detection method for a newly revealed mechanism of pyrethroid resistance development in Varroa destructor in PARASITOLOGY RESEARCH
  • 2013-03. Proteolysis on the body surface of pyrethroid-sensitive and resistant Varroa destructor in ACTA PARASITOLOGICA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10493-019-00353-9

    DOI

    http://dx.doi.org/10.1007/s10493-019-00353-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112400494

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30810851


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