A novel strategy to enhance angiogenesis in vivo using the small VEGF-binding peptide PR1P View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-08

AUTHORS

Avner Adini, Irit Adini, Zai-long Chi, Ratmir Derda, Amy E. Birsner, Benjamin D. Matthews, Robert J. D’Amato

ABSTRACT

Therapeutic angiogenesis is an experimental frontier in vascular biology that seeks to deliver angiogenic growth factors to ischemic or injured tissues to promote targeted formation of new blood vessels as an alternative approach to surgical revascularization procedures. Vascular endothelial growth factor (VEGF) is a potent angiogenic signal protein that is locally upregulated at sites of tissue injury. However, therapies aimed at increasing VEGF levels experimentally by injecting VEGF gene or protein failed to improve outcomes in human trials in part due to its short half-life and systemic toxicity. We recently designed a novel 12-amino acid peptide (PR1P) whose sequence was derived from an extracellular VEGF-binding domain of the pro-angiogenic glycoprotein prominin-1. In this study, we characterized the molecular binding properties of this novel potential therapeutic for targeted angiogenesis and provided the foundation for its use as an angiogenic molecule that can potentiate endogenous VEGF. We showed that PR1P bound VEGF directly and enhanced VEGF binding to endothelial cells and to VEGF receptors VEGFR2 and neuropilin-1. PR1P increased angiogenesis in the murine corneal micropocket assay when combined with VEGF, but had no activity without added VEGF. In addition, PR1P also enhanced angiogenesis in murine choroidal neovascularization and wound-healing models and augmented reperfusion in a murine hind-limb ischemia model. Together our data suggest that PR1P enhanced angiogenesis by potentiating the activity of endogenous VEGF. In so doing, this novel therapy takes advantage of endogenous VEGF gradients generated in injured tissues and may improve the efficacy of and avoid systemic toxicity seen with previous VEGF therapies. More... »

PAGES

399-408

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10456-017-9556-7

DOI

http://dx.doi.org/10.1007/s10456-017-9556-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084767110

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28397127


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Angiogenesis Inducing Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ischemia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neovascularization, Physiologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Fragments", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Perfusion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vascular Endothelial Growth Factor A", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA", 
            "Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Adini", 
        "givenName": "Avner", 
        "id": "sg:person.01207765422.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207765422.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Adini", 
        "givenName": "Irit", 
        "id": "sg:person.01076307441.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076307441.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chi", 
        "givenName": "Zai-long", 
        "id": "sg:person.012536421377.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012536421377.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Alberta", 
          "id": "https://www.grid.ac/institutes/grid.17089.37", 
          "name": [
            "Department of Chemistry, University of Alberta, Edmonton, AB, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Derda", 
        "givenName": "Ratmir", 
        "id": "sg:person.01114114477.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114114477.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Birsner", 
        "givenName": "Amy E.", 
        "id": "sg:person.01223100751.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01223100751.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA", 
            "Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matthews", 
        "givenName": "Benjamin D.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Harvard University", 
          "id": "https://www.grid.ac/institutes/grid.38142.3c", 
          "name": [
            "Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, Harvard Medical School, 02115, Boston, MA, USA", 
            "Department of Ophthalmology, Harvard Medical School, 02115, Boston, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "D\u2019Amato", 
        "givenName": "Robert J.", 
        "id": "sg:person.0603360341.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603360341.41"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1161/01.cir.102.8.898", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000196400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/gt.2011.66", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003966131", 
          "https://doi.org/10.1038/gt.2011.66"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-59259-453-5_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007969689", 
          "https://doi.org/10.1007/978-1-59259-453-5_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1247/csf.26.25", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009161956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1046/j.1523-1747.2000.00036.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011185890", 
          "https://doi.org/10.1046/j.1523-1747.2000.00036.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci35891", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011371661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1023403928385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015581600", 
          "https://doi.org/10.1023/a:1023403928385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10456-012-9323-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016007982", 
          "https://doi.org/10.1007/s10456-012-9323-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jss.2005.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019095233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/wsbm.92", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019704559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jss.2008.04.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019942638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000019540.41697.60", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020525306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.08-124321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022978652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1582-4934.2006.tb00290.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027504951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01739641", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027664151", 
          "https://doi.org/10.1007/bf01739641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01739641", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027664151", 
          "https://doi.org/10.1007/bf01739641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05249", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029773877", 
          "https://doi.org/10.1038/nature05249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05249", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029773877", 
          "https://doi.org/10.1038/nature05249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0603-669", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031340920", 
          "https://doi.org/10.1038/nm0603-669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0603-669", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031340920", 
          "https://doi.org/10.1038/nm0603-669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1167/iovs.09-4561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031827376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2007.368", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041299540", 
          "https://doi.org/10.1038/nprot.2007.368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.2002.2013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043199390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(02)03545-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045890389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jvs.2004.11.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047142965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.cir.0000061911.47710.8a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051232689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/gt.2012.17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052126353", 
          "https://doi.org/10.1038/gt.2012.17"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4062/biomolther.2013.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052274821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.2006.17.683", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059272810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/wound.2013.0517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059327923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/er.2003-0027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064285794"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7326/0003-4819-132-11-200006060-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1073703786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074713871", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076984953", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077934148", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078332010", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082909404", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-08", 
    "datePublishedReg": "2017-08-01", 
    "description": "Therapeutic angiogenesis is an experimental frontier in vascular biology that seeks to deliver angiogenic growth factors to ischemic or injured tissues to promote targeted formation of new blood vessels as an alternative approach to surgical revascularization procedures. Vascular endothelial growth factor (VEGF) is a potent angiogenic signal protein that is locally upregulated at sites of tissue injury. However, therapies aimed at increasing VEGF levels experimentally by injecting VEGF gene or protein failed to improve outcomes in human trials in part due to its short half-life and systemic toxicity. We recently designed a novel 12-amino acid peptide (PR1P) whose sequence was derived from an extracellular VEGF-binding domain of the pro-angiogenic glycoprotein prominin-1. In this study, we characterized the molecular binding properties of this novel potential therapeutic for targeted angiogenesis and provided the foundation for its use as an angiogenic molecule that can potentiate endogenous VEGF. We showed that PR1P bound VEGF directly and enhanced VEGF binding to endothelial cells and to VEGF receptors VEGFR2 and neuropilin-1. PR1P increased angiogenesis in the murine corneal micropocket assay when combined with VEGF, but had no activity without added VEGF. In addition, PR1P also enhanced angiogenesis in murine choroidal neovascularization and wound-healing models and augmented reperfusion in a murine hind-limb ischemia model. Together our data suggest that PR1P enhanced angiogenesis by potentiating the activity of endogenous VEGF. In so doing, this novel therapy takes advantage of endogenous VEGF gradients generated in injured tissues and may improve the efficacy of and avoid systemic toxicity seen with previous VEGF therapies.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10456-017-9556-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1119026", 
        "issn": [
          "0969-6970", 
          "1573-7209"
        ], 
        "name": "Angiogenesis", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "20"
      }
    ], 
    "name": "A novel strategy to enhance angiogenesis in vivo using the small VEGF-binding peptide PR1P", 
    "pagination": "399-408", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bae9676848e71332986a05fbded45194c44c0d8f5861212110a3350b230ecd1e"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28397127"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9814575"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10456-017-9556-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084767110"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10456-017-9556-7", 
      "https://app.dimensions.ai/details/publication/pub.1084767110"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113670_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs10456-017-9556-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10456-017-9556-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10456-017-9556-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10456-017-9556-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10456-017-9556-7'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      75 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10456-017-9556-7 schema:about N1e8592b1e51b481a8c355ee791a73687
2 N26c53113509545f881994e0cec5569c5
3 N5d0513d18a984ff7b66db8a23089be33
4 N63d418a06f774eaea9892a399afad276
5 N6415ea3c9d3d4a99b1cb8b29679e3bc0
6 N7fdb55e766484c3db16feaed81cf2a1c
7 N8a362ab0d4204ec6ae9368c22726f2c1
8 N8f419ce45f254aa9a27aa60b2a619fbb
9 N968f8b6670cf473081e50c6bf8b57697
10 N96d58b95f4124364aff2ef0cbbf25cae
11 Neaef8c2331c649d3b722a7f1990a1178
12 Nee60195abc48449baa53c154c7595bd1
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N0e7f68fb65aa4015a7994cbdb920d37e
16 schema:citation sg:pub.10.1007/978-1-59259-453-5_8
17 sg:pub.10.1007/bf01739641
18 sg:pub.10.1007/s10456-012-9323-8
19 sg:pub.10.1023/a:1023403928385
20 sg:pub.10.1038/gt.2011.66
21 sg:pub.10.1038/gt.2012.17
22 sg:pub.10.1038/nature05249
23 sg:pub.10.1038/nm0603-669
24 sg:pub.10.1038/nprot.2007.368
25 sg:pub.10.1046/j.1523-1747.2000.00036.x
26 https://app.dimensions.ai/details/publication/pub.1074713871
27 https://app.dimensions.ai/details/publication/pub.1076984953
28 https://app.dimensions.ai/details/publication/pub.1077934148
29 https://app.dimensions.ai/details/publication/pub.1078332010
30 https://app.dimensions.ai/details/publication/pub.1082909404
31 https://doi.org/10.1002/wsbm.92
32 https://doi.org/10.1006/jmcc.2002.2013
33 https://doi.org/10.1016/j.jss.2005.12.003
34 https://doi.org/10.1016/j.jss.2008.04.023
35 https://doi.org/10.1016/j.jvs.2004.11.012
36 https://doi.org/10.1016/s0014-5793(02)03545-7
37 https://doi.org/10.1089/hum.2006.17.683
38 https://doi.org/10.1089/wound.2013.0517
39 https://doi.org/10.1096/fj.08-124321
40 https://doi.org/10.1111/j.1582-4934.2006.tb00290.x
41 https://doi.org/10.1161/01.cir.0000061911.47710.8a
42 https://doi.org/10.1161/01.cir.102.8.898
43 https://doi.org/10.1161/01.res.0000019540.41697.60
44 https://doi.org/10.1167/iovs.09-4561
45 https://doi.org/10.1172/jci35891
46 https://doi.org/10.1210/er.2003-0027
47 https://doi.org/10.1247/csf.26.25
48 https://doi.org/10.4062/biomolther.2013.113
49 https://doi.org/10.7326/0003-4819-132-11-200006060-00005
50 schema:datePublished 2017-08
51 schema:datePublishedReg 2017-08-01
52 schema:description Therapeutic angiogenesis is an experimental frontier in vascular biology that seeks to deliver angiogenic growth factors to ischemic or injured tissues to promote targeted formation of new blood vessels as an alternative approach to surgical revascularization procedures. Vascular endothelial growth factor (VEGF) is a potent angiogenic signal protein that is locally upregulated at sites of tissue injury. However, therapies aimed at increasing VEGF levels experimentally by injecting VEGF gene or protein failed to improve outcomes in human trials in part due to its short half-life and systemic toxicity. We recently designed a novel 12-amino acid peptide (PR1P) whose sequence was derived from an extracellular VEGF-binding domain of the pro-angiogenic glycoprotein prominin-1. In this study, we characterized the molecular binding properties of this novel potential therapeutic for targeted angiogenesis and provided the foundation for its use as an angiogenic molecule that can potentiate endogenous VEGF. We showed that PR1P bound VEGF directly and enhanced VEGF binding to endothelial cells and to VEGF receptors VEGFR2 and neuropilin-1. PR1P increased angiogenesis in the murine corneal micropocket assay when combined with VEGF, but had no activity without added VEGF. In addition, PR1P also enhanced angiogenesis in murine choroidal neovascularization and wound-healing models and augmented reperfusion in a murine hind-limb ischemia model. Together our data suggest that PR1P enhanced angiogenesis by potentiating the activity of endogenous VEGF. In so doing, this novel therapy takes advantage of endogenous VEGF gradients generated in injured tissues and may improve the efficacy of and avoid systemic toxicity seen with previous VEGF therapies.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree false
56 schema:isPartOf N7fe8d9f1892d451e8d4680e359e7c8a4
57 Ne631111efa1f491586d7ffeaa923368c
58 sg:journal.1119026
59 schema:name A novel strategy to enhance angiogenesis in vivo using the small VEGF-binding peptide PR1P
60 schema:pagination 399-408
61 schema:productId N3046d1a808434bf587c55fec8a332bd6
62 N6a675194a77640c588acb5b4a42464d0
63 N930a5c5bf52d4b549da8b048918338ca
64 Nd1c2b1dea5454becba72f28cb812077d
65 Nfddd6b989f0f444fbecee2a364174502
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084767110
67 https://doi.org/10.1007/s10456-017-9556-7
68 schema:sdDatePublished 2019-04-11T10:36
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N1ad10bb6fcc946b2bc8572e077e9deb1
71 schema:url https://link.springer.com/10.1007%2Fs10456-017-9556-7
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N0e7f68fb65aa4015a7994cbdb920d37e rdf:first sg:person.01207765422.54
76 rdf:rest Nc062edac18034818b29fa3189a4c9d40
77 N1ad10bb6fcc946b2bc8572e077e9deb1 schema:name Springer Nature - SN SciGraph project
78 rdf:type schema:Organization
79 N1c09c438981b43a6983d8a70be292b5d schema:affiliation N96a9f42dbd744be587c1576d4e6df2b1
80 schema:familyName Matthews
81 schema:givenName Benjamin D.
82 rdf:type schema:Person
83 N1e8592b1e51b481a8c355ee791a73687 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Protein Binding
85 rdf:type schema:DefinedTerm
86 N1f0b0955b04a4fa69010d7c635347c55 rdf:first N1c09c438981b43a6983d8a70be292b5d
87 rdf:rest Nb01d47b0ec444622a64b7e6cd19de4eb
88 N26c53113509545f881994e0cec5569c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Animals
90 rdf:type schema:DefinedTerm
91 N29a2f1896ea5411fad305a453a5f1016 rdf:first sg:person.012536421377.62
92 rdf:rest Nc4291c899c1d4474b17474db87da8d61
93 N3046d1a808434bf587c55fec8a332bd6 schema:name dimensions_id
94 schema:value pub.1084767110
95 rdf:type schema:PropertyValue
96 N42f3ac18c4b248d692ab9967f8715a6d schema:name Vascular Biology Program, Department of Surgery, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
97 rdf:type schema:Organization
98 N5d0513d18a984ff7b66db8a23089be33 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Perfusion
100 rdf:type schema:DefinedTerm
101 N63d418a06f774eaea9892a399afad276 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Peptides
103 rdf:type schema:DefinedTerm
104 N6415ea3c9d3d4a99b1cb8b29679e3bc0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Peptide Fragments
106 rdf:type schema:DefinedTerm
107 N6a675194a77640c588acb5b4a42464d0 schema:name readcube_id
108 schema:value bae9676848e71332986a05fbded45194c44c0d8f5861212110a3350b230ecd1e
109 rdf:type schema:PropertyValue
110 N6d2157d3e01647a681d9e04137f84893 schema:name Vascular Biology Program, Department of Surgery, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
111 rdf:type schema:Organization
112 N7fdb55e766484c3db16feaed81cf2a1c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Ischemia
114 rdf:type schema:DefinedTerm
115 N7fe8d9f1892d451e8d4680e359e7c8a4 schema:issueNumber 3
116 rdf:type schema:PublicationIssue
117 N82ab6f699003407b938b92106deedafc schema:name Department of Medicine, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
118 Vascular Biology Program, Department of Surgery, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
119 rdf:type schema:Organization
120 N8a362ab0d4204ec6ae9368c22726f2c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Female
122 rdf:type schema:DefinedTerm
123 N8f419ce45f254aa9a27aa60b2a619fbb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Neovascularization, Physiologic
125 rdf:type schema:DefinedTerm
126 N930a5c5bf52d4b549da8b048918338ca schema:name nlm_unique_id
127 schema:value 9814575
128 rdf:type schema:PropertyValue
129 N937bf962da4a4161811826c9150b578d schema:name Vascular Biology Program, Department of Surgery, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
130 rdf:type schema:Organization
131 N968f8b6670cf473081e50c6bf8b57697 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Mice, Inbred C57BL
133 rdf:type schema:DefinedTerm
134 N96a9f42dbd744be587c1576d4e6df2b1 schema:name Department of Medicine, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
135 Vascular Biology Program, Department of Surgery, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
136 rdf:type schema:Organization
137 N96d58b95f4124364aff2ef0cbbf25cae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Humans
139 rdf:type schema:DefinedTerm
140 Nb01d47b0ec444622a64b7e6cd19de4eb rdf:first sg:person.0603360341.41
141 rdf:rest rdf:nil
142 Nc062edac18034818b29fa3189a4c9d40 rdf:first sg:person.01076307441.22
143 rdf:rest N29a2f1896ea5411fad305a453a5f1016
144 Nc4291c899c1d4474b17474db87da8d61 rdf:first sg:person.01114114477.64
145 rdf:rest Neb12bc73efc44a1ca068c5ba265cc1fe
146 Nd1c2b1dea5454becba72f28cb812077d schema:name pubmed_id
147 schema:value 28397127
148 rdf:type schema:PropertyValue
149 Ne631111efa1f491586d7ffeaa923368c schema:volumeNumber 20
150 rdf:type schema:PublicationVolume
151 Neaef8c2331c649d3b722a7f1990a1178 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Angiogenesis Inducing Agents
153 rdf:type schema:DefinedTerm
154 Neb12bc73efc44a1ca068c5ba265cc1fe rdf:first sg:person.01223100751.10
155 rdf:rest N1f0b0955b04a4fa69010d7c635347c55
156 Nee60195abc48449baa53c154c7595bd1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Vascular Endothelial Growth Factor A
158 rdf:type schema:DefinedTerm
159 Nfddd6b989f0f444fbecee2a364174502 schema:name doi
160 schema:value 10.1007/s10456-017-9556-7
161 rdf:type schema:PropertyValue
162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biological Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
166 schema:name Biochemistry and Cell Biology
167 rdf:type schema:DefinedTerm
168 sg:journal.1119026 schema:issn 0969-6970
169 1573-7209
170 schema:name Angiogenesis
171 rdf:type schema:Periodical
172 sg:person.01076307441.22 schema:affiliation N42f3ac18c4b248d692ab9967f8715a6d
173 schema:familyName Adini
174 schema:givenName Irit
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076307441.22
176 rdf:type schema:Person
177 sg:person.01114114477.64 schema:affiliation https://www.grid.ac/institutes/grid.17089.37
178 schema:familyName Derda
179 schema:givenName Ratmir
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114114477.64
181 rdf:type schema:Person
182 sg:person.01207765422.54 schema:affiliation N82ab6f699003407b938b92106deedafc
183 schema:familyName Adini
184 schema:givenName Avner
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207765422.54
186 rdf:type schema:Person
187 sg:person.01223100751.10 schema:affiliation N937bf962da4a4161811826c9150b578d
188 schema:familyName Birsner
189 schema:givenName Amy E.
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01223100751.10
191 rdf:type schema:Person
192 sg:person.012536421377.62 schema:affiliation N6d2157d3e01647a681d9e04137f84893
193 schema:familyName Chi
194 schema:givenName Zai-long
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012536421377.62
196 rdf:type schema:Person
197 sg:person.0603360341.41 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
198 schema:familyName D’Amato
199 schema:givenName Robert J.
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603360341.41
201 rdf:type schema:Person
202 sg:pub.10.1007/978-1-59259-453-5_8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007969689
203 https://doi.org/10.1007/978-1-59259-453-5_8
204 rdf:type schema:CreativeWork
205 sg:pub.10.1007/bf01739641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027664151
206 https://doi.org/10.1007/bf01739641
207 rdf:type schema:CreativeWork
208 sg:pub.10.1007/s10456-012-9323-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016007982
209 https://doi.org/10.1007/s10456-012-9323-8
210 rdf:type schema:CreativeWork
211 sg:pub.10.1023/a:1023403928385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015581600
212 https://doi.org/10.1023/a:1023403928385
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/gt.2011.66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003966131
215 https://doi.org/10.1038/gt.2011.66
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/gt.2012.17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052126353
218 https://doi.org/10.1038/gt.2012.17
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/nature05249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029773877
221 https://doi.org/10.1038/nature05249
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/nm0603-669 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031340920
224 https://doi.org/10.1038/nm0603-669
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/nprot.2007.368 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041299540
227 https://doi.org/10.1038/nprot.2007.368
228 rdf:type schema:CreativeWork
229 sg:pub.10.1046/j.1523-1747.2000.00036.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011185890
230 https://doi.org/10.1046/j.1523-1747.2000.00036.x
231 rdf:type schema:CreativeWork
232 https://app.dimensions.ai/details/publication/pub.1074713871 schema:CreativeWork
233 https://app.dimensions.ai/details/publication/pub.1076984953 schema:CreativeWork
234 https://app.dimensions.ai/details/publication/pub.1077934148 schema:CreativeWork
235 https://app.dimensions.ai/details/publication/pub.1078332010 schema:CreativeWork
236 https://app.dimensions.ai/details/publication/pub.1082909404 schema:CreativeWork
237 https://doi.org/10.1002/wsbm.92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019704559
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1006/jmcc.2002.2013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043199390
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.jss.2005.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019095233
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.jss.2008.04.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019942638
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.jvs.2004.11.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047142965
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/s0014-5793(02)03545-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045890389
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1089/hum.2006.17.683 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272810
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1089/wound.2013.0517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059327923
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1096/fj.08-124321 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022978652
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1111/j.1582-4934.2006.tb00290.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027504951
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1161/01.cir.0000061911.47710.8a schema:sameAs https://app.dimensions.ai/details/publication/pub.1051232689
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1161/01.cir.102.8.898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000196400
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1161/01.res.0000019540.41697.60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020525306
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1167/iovs.09-4561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031827376
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1172/jci35891 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011371661
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1210/er.2003-0027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064285794
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1247/csf.26.25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009161956
270 rdf:type schema:CreativeWork
271 https://doi.org/10.4062/biomolther.2013.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052274821
272 rdf:type schema:CreativeWork
273 https://doi.org/10.7326/0003-4819-132-11-200006060-00005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1073703786
274 rdf:type schema:CreativeWork
275 https://www.grid.ac/institutes/grid.17089.37 schema:alternateName University of Alberta
276 schema:name Department of Chemistry, University of Alberta, Edmonton, AB, Canada
277 rdf:type schema:Organization
278 https://www.grid.ac/institutes/grid.38142.3c schema:alternateName Harvard University
279 schema:name Department of Ophthalmology, Harvard Medical School, 02115, Boston, MA, USA
280 Vascular Biology Program, Department of Surgery, Boston Children’s Hospital, Harvard Medical School, 02115, Boston, MA, USA
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...