Microbial hitchhikers on intercontinental dust: high-throughput sequencing to catalogue microbes in small sand samples View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-03

AUTHORS

Adriana Giongo, Jocelyne Favet, Ales Lapanje, Kelsey A. Gano, Suzanne Kennedy, Austin G. Davis-Richardson, Christopher Brown, Andreas Beck, William G. Farmerie, Arlette Cattaneo, David B. Crabb, Yin-Yin Aung, Renate Kort, Hans-Jürgen Brumsack, Bernhard Schnetger, William J. Broughton, Anna A. Gorbushina, Eric W. Triplett

ABSTRACT

Microbiological studies on the intercontinental transport of dust are confounded by the difficulty of obtaining sufficient material for analysis. Axenic samples of dust collected at high altitudes or historic specimens in museums are often so small and precious that the material can only be sacrificed when positive results are assured. With this in mind, we evaluated current methods and developed new ones in an attempt to catalogue all microbes present in small dust or sand samples. The methods used included classical microbiological approaches in which sand extracts were plated out on a variety of different media, polymerase chain reaction (PCR)-based amplification of 16S/18S rRNA sequences followed by construction of clone libraries, PCR amplification of 16S rRNA sequences followed by high-throughput sequencing (HtS) of the products and direct HtS of DNA extracted from the sand. A representative sand sample collected at Bahaï Wadi in the desert of the Republic of Chad was used. HtS with or without amplification showed the most promise and can be performed on ≤100 ng DNA. Since living microbes are often required, current best practices would involve geochemical and microscopic characterisation of the sample, followed by DNA isolation and direct HtS. Once the microbial content of the sample has been deciphered, growth conditions (including media) can be tailored to isolate the micro-organisms of interest. More... »

PAGES

71-84

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10453-012-9264-0

DOI

http://dx.doi.org/10.1007/s10453-012-9264-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027904064


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Giongo", 
        "givenName": "Adriana", 
        "id": "sg:person.01113221153.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113221153.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Geneva", 
          "id": "https://www.grid.ac/institutes/grid.8591.5", 
          "name": [
            "Universit\u00e9 de Gen\u00e8ve, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Favet", 
        "givenName": "Jocelyne", 
        "id": "sg:person.0707007074.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707007074.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Physical Biology, Toplarniska 19, 1000, Ljubljana, Slovenia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lapanje", 
        "givenName": "Ales", 
        "id": "sg:person.01154200725.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154200725.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gano", 
        "givenName": "Kelsey A.", 
        "id": "sg:person.01072431277.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072431277.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "MO BIO Laboratories, Inc., 2746 Loker Ave. West, 92010, Carlsbad, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kennedy", 
        "givenName": "Suzanne", 
        "id": "sg:person.01071350674.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071350674.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davis-Richardson", 
        "givenName": "Austin G.", 
        "id": "sg:person.01024316077.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024316077.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brown", 
        "givenName": "Christopher", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Lichenology and Bryology, Botanische Staatssammlung M\u00fcnchen, Menzinger Stra\u00dfe 67, 80638, Munich, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beck", 
        "givenName": "Andreas", 
        "id": "sg:person.01027402034.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027402034.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, 2033 Mowry Road, 32610, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Farmerie", 
        "givenName": "William G.", 
        "id": "sg:person.0575364545.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575364545.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Geneva", 
          "id": "https://www.grid.ac/institutes/grid.8591.5", 
          "name": [
            "Universit\u00e9 de Gen\u00e8ve, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cattaneo", 
        "givenName": "Arlette", 
        "id": "sg:person.01004257645.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01004257645.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crabb", 
        "givenName": "David B.", 
        "id": "sg:person.01227447553.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227447553.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Geneva", 
          "id": "https://www.grid.ac/institutes/grid.8591.5", 
          "name": [
            "Universit\u00e9 de Gen\u00e8ve, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aung", 
        "givenName": "Yin-Yin", 
        "id": "sg:person.01137464074.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137464074.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Carl von Ossietzky University of Oldenburg", 
          "id": "https://www.grid.ac/institutes/grid.5560.6", 
          "name": [
            "Elektronenmikroskopie, Carl von Ossietzky Universit\u00e4t, Oldenburg, Carl-von-Ossietzky str. 9-11, 26111, Oldenburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kort", 
        "givenName": "Renate", 
        "id": "sg:person.01370141074.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370141074.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Carl von Ossietzky University of Oldenburg", 
          "id": "https://www.grid.ac/institutes/grid.5560.6", 
          "name": [
            "Microbiogeochemie, ICBM, Carl von Ossietzky Universit\u00e4t, Oldenburg, Carl-von-Ossietzky str. 9-11, 26111, Oldenburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brumsack", 
        "givenName": "Hans-J\u00fcrgen", 
        "id": "sg:person.0576725034.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576725034.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Carl von Ossietzky University of Oldenburg", 
          "id": "https://www.grid.ac/institutes/grid.5560.6", 
          "name": [
            "Microbiogeochemie, ICBM, Carl von Ossietzky Universit\u00e4t, Oldenburg, Carl-von-Ossietzky str. 9-11, 26111, Oldenburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schnetger", 
        "givenName": "Bernhard", 
        "id": "sg:person.0645040234.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645040234.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Geneva", 
          "id": "https://www.grid.ac/institutes/grid.8591.5", 
          "name": [
            "Universit\u00e9 de Gen\u00e8ve, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland", 
            "Abteilung Material und Umwelt, Bundesanstalt f\u00fcr Material-forschung, und -pr\u00fcfung, Fabeckstrasse 62 A, 14195, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Broughton", 
        "givenName": "William J.", 
        "id": "sg:person.01364017155.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364017155.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Freie Universit\u00e4t Berlin", 
          "id": "https://www.grid.ac/institutes/grid.14095.39", 
          "name": [
            "Universit\u00e9 de Gen\u00e8ve, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland", 
            "Abteilung Material und Umwelt, Bundesanstalt f\u00fcr Material-forschung, und -pr\u00fcfung, Fabeckstrasse 62 A, 14195, Berlin, Germany", 
            "Fachbereich Biologie, Chemie und Pharmazie & Geowissenschaften, Freie Universit\u00e4t Berlin, Malteserstrasse 74-100, 12249, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gorbushina", 
        "givenName": "Anna A.", 
        "id": "sg:person.0704675400.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704675400.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Triplett", 
        "givenName": "Eric W.", 
        "id": "sg:person.01207666013.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207666013.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.3390/d2071015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000307020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0303-2647(78)90023-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005696076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0303-2647(78)90023-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005696076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.earscirev.2006.06.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006545737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1440-1835.2010.00579.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009568861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1440-1835.2010.00579.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009568861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.1390403", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014635681"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.01526-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014766903"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ibiod.2003.07.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020343326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2012.152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023561351", 
          "https://doi.org/10.1038/ismej.2012.152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1251075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026778829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1251075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026778829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tree.2006.07.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031629347"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031640134", 
          "https://doi.org/10.1038/nrmicro1160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031640134", 
          "https://doi.org/10.1038/nrmicro1160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031640134", 
          "https://doi.org/10.1038/nrmicro1160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10453-010-9173-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033578497", 
          "https://doi.org/10.1007/s10453-010-9173-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.00039-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034554429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10453-011-9230-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036246667", 
          "https://doi.org/10.1007/s10453-011-9230-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.atmosenv.2007.01.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038551242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(99)00154-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038625209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.aeolia.2011.02.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039743600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/434816a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040879530", 
          "https://doi.org/10.1038/434816a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/434816a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040879530", 
          "https://doi.org/10.1038/434816a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkn879", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044918953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.envpol.2010.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045958676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.soilbio.2010.02.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049545348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2010.16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050994807", 
          "https://doi.org/10.1038/ismej.2010.16"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-2920.2007.01461.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051265957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002160050614", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051702407", 
          "https://doi.org/10.1007/s002160050614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi.1999.12.4.293", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060080067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080013201", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-03", 
    "datePublishedReg": "2013-03-01", 
    "description": "Microbiological studies on the intercontinental transport of dust are confounded by the difficulty of obtaining sufficient material for analysis. Axenic samples of dust collected at high altitudes or historic specimens in museums are often so small and precious that the material can only be sacrificed when positive results are assured. With this in mind, we evaluated current methods and developed new ones in an attempt to catalogue all microbes present in small dust or sand samples. The methods used included classical microbiological approaches in which sand extracts were plated out on a variety of different media, polymerase chain reaction (PCR)-based amplification of 16S/18S rRNA sequences followed by construction of clone libraries, PCR amplification of 16S rRNA sequences followed by high-throughput sequencing (HtS) of the products and direct HtS of DNA extracted from the sand. A representative sand sample collected at Baha\u00ef Wadi in the desert of the Republic of Chad was used. HtS with or without amplification showed the most promise and can be performed on \u2264100 ng DNA. Since living microbes are often required, current best practices would involve geochemical and microscopic characterisation of the sample, followed by DNA isolation and direct HtS. Once the microbial content of the sample has been deciphered, growth conditions (including media) can be tailored to isolate the micro-organisms of interest.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10453-012-9264-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3055441", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1133886", 
        "issn": [
          "0393-5965", 
          "1573-3025"
        ], 
        "name": "Aerobiologia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "29"
      }
    ], 
    "name": "Microbial hitchhikers on intercontinental dust: high-throughput sequencing to catalogue microbes in small sand samples", 
    "pagination": "71-84", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "84ed0041a0ecd77c10d0f5711d08b44a00be6b87f654b1ac9983bd68ce22bb07"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10453-012-9264-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027904064"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10453-012-9264-0", 
      "https://app.dimensions.ai/details/publication/pub.1027904064"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000513.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs10453-012-9264-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10453-012-9264-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10453-012-9264-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10453-012-9264-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10453-012-9264-0'


 

This table displays all metadata directly associated to this object as RDF triples.

285 TRIPLES      21 PREDICATES      53 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10453-012-9264-0 schema:about anzsrc-for:06
2 anzsrc-for:0605
3 schema:author Nedb578786a9b4763a77f8e4d80c2604d
4 schema:citation sg:pub.10.1007/s002160050614
5 sg:pub.10.1007/s10453-010-9173-z
6 sg:pub.10.1007/s10453-011-9230-2
7 sg:pub.10.1038/434816a
8 sg:pub.10.1038/ismej.2010.16
9 sg:pub.10.1038/ismej.2012.152
10 sg:pub.10.1038/nrmicro1160
11 https://app.dimensions.ai/details/publication/pub.1080013201
12 https://doi.org/10.1016/0303-2647(78)90023-0
13 https://doi.org/10.1016/j.aeolia.2011.02.001
14 https://doi.org/10.1016/j.atmosenv.2007.01.023
15 https://doi.org/10.1016/j.earscirev.2006.06.004
16 https://doi.org/10.1016/j.envpol.2010.07.001
17 https://doi.org/10.1016/j.ibiod.2003.07.003
18 https://doi.org/10.1016/j.soilbio.2010.02.019
19 https://doi.org/10.1016/j.tree.2006.07.004
20 https://doi.org/10.1016/s0168-1656(99)00154-6
21 https://doi.org/10.1042/bj1251075
22 https://doi.org/10.1093/nar/gkn879
23 https://doi.org/10.1094/mpmi.1999.12.4.293
24 https://doi.org/10.1101/gr.1390403
25 https://doi.org/10.1111/j.1440-1835.2010.00579.x
26 https://doi.org/10.1111/j.1462-2920.2007.01461.x
27 https://doi.org/10.1128/aem.01526-10
28 https://doi.org/10.1128/cmr.00039-06
29 https://doi.org/10.3390/d2071015
30 schema:datePublished 2013-03
31 schema:datePublishedReg 2013-03-01
32 schema:description Microbiological studies on the intercontinental transport of dust are confounded by the difficulty of obtaining sufficient material for analysis. Axenic samples of dust collected at high altitudes or historic specimens in museums are often so small and precious that the material can only be sacrificed when positive results are assured. With this in mind, we evaluated current methods and developed new ones in an attempt to catalogue all microbes present in small dust or sand samples. The methods used included classical microbiological approaches in which sand extracts were plated out on a variety of different media, polymerase chain reaction (PCR)-based amplification of 16S/18S rRNA sequences followed by construction of clone libraries, PCR amplification of 16S rRNA sequences followed by high-throughput sequencing (HtS) of the products and direct HtS of DNA extracted from the sand. A representative sand sample collected at Bahaï Wadi in the desert of the Republic of Chad was used. HtS with or without amplification showed the most promise and can be performed on ≤100 ng DNA. Since living microbes are often required, current best practices would involve geochemical and microscopic characterisation of the sample, followed by DNA isolation and direct HtS. Once the microbial content of the sample has been deciphered, growth conditions (including media) can be tailored to isolate the micro-organisms of interest.
33 schema:genre research_article
34 schema:inLanguage en
35 schema:isAccessibleForFree true
36 schema:isPartOf N21eff173193e44219c1a838aa63995e2
37 N9a8cfcac81d6420fbad61f26994e40ba
38 sg:journal.1133886
39 schema:name Microbial hitchhikers on intercontinental dust: high-throughput sequencing to catalogue microbes in small sand samples
40 schema:pagination 71-84
41 schema:productId N7f385a9e072d482385ab8d90a20e95bf
42 Nc74c645ef3e94331bf1d3492b4679538
43 Nd874dd8fd6384919915de42d6313d281
44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027904064
45 https://doi.org/10.1007/s10453-012-9264-0
46 schema:sdDatePublished 2019-04-10T17:32
47 schema:sdLicense https://scigraph.springernature.com/explorer/license/
48 schema:sdPublisher Ndcd583cdc3294514aecd2303fb332013
49 schema:url http://link.springer.com/10.1007%2Fs10453-012-9264-0
50 sgo:license sg:explorer/license/
51 sgo:sdDataset articles
52 rdf:type schema:ScholarlyArticle
53 N089d629bfcb8424e9c4abb83795f6c0d rdf:first sg:person.01364017155.92
54 rdf:rest Nfe29e2bcd3ac475f8bc9c6c8eefe0035
55 N0d07b14bd83a40a49b1b816709aff42c schema:affiliation https://www.grid.ac/institutes/grid.15276.37
56 schema:familyName Brown
57 schema:givenName Christopher
58 rdf:type schema:Person
59 N10c1c544989246e1a84df60b0a9ca49c schema:name Department of Lichenology and Bryology, Botanische Staatssammlung München, Menzinger Straße 67, 80638, Munich, Germany
60 rdf:type schema:Organization
61 N1cfc23dceadb472492a84ea16056500f rdf:first sg:person.01227447553.36
62 rdf:rest Ncde716e3eb48450dbe66a7eb1e82b735
63 N21eff173193e44219c1a838aa63995e2 schema:volumeNumber 29
64 rdf:type schema:PublicationVolume
65 N293d1229bafa4c31b6b2f2b6c892f9c2 rdf:first sg:person.01004257645.86
66 rdf:rest N1cfc23dceadb472492a84ea16056500f
67 N3e580e2a8cca425e9b4f30cfb6657218 rdf:first sg:person.0576725034.96
68 rdf:rest Nebb80a05083749bc9e1be29ca9b1f9dd
69 N435c348f817748f39f62e0ad5c0ce930 schema:name MO BIO Laboratories, Inc., 2746 Loker Ave. West, 92010, Carlsbad, CA, USA
70 rdf:type schema:Organization
71 N5380eb5ba2a74e13bc8d3c4f710464be rdf:first sg:person.01071350674.20
72 rdf:rest N96a04a1c4cdf427bb71f89c86e173ed1
73 N5a6cd80338fa4268829570fd4162cd41 rdf:first sg:person.01027402034.57
74 rdf:rest N875ed3f10f9f436db0f2375cc3529446
75 N5c9b1f3d939d4d63a3ebad2330649e38 rdf:first sg:person.01370141074.27
76 rdf:rest N3e580e2a8cca425e9b4f30cfb6657218
77 N61a9e04a88d3444fa002ec02b80b59af rdf:first N0d07b14bd83a40a49b1b816709aff42c
78 rdf:rest N5a6cd80338fa4268829570fd4162cd41
79 N7f385a9e072d482385ab8d90a20e95bf schema:name readcube_id
80 schema:value 84ed0041a0ecd77c10d0f5711d08b44a00be6b87f654b1ac9983bd68ce22bb07
81 rdf:type schema:PropertyValue
82 N875ed3f10f9f436db0f2375cc3529446 rdf:first sg:person.0575364545.05
83 rdf:rest N293d1229bafa4c31b6b2f2b6c892f9c2
84 N96092b7c652649da932fc95a61308f7f rdf:first sg:person.01072431277.90
85 rdf:rest N5380eb5ba2a74e13bc8d3c4f710464be
86 N96a04a1c4cdf427bb71f89c86e173ed1 rdf:first sg:person.01024316077.25
87 rdf:rest N61a9e04a88d3444fa002ec02b80b59af
88 N9a8cfcac81d6420fbad61f26994e40ba schema:issueNumber 1
89 rdf:type schema:PublicationIssue
90 Na3c0b99137f146108545b9499bf64f7d rdf:first sg:person.0707007074.06
91 rdf:rest Nf23bd6e136894b5cbcd1ccd04f7ba0ec
92 Nc74c645ef3e94331bf1d3492b4679538 schema:name dimensions_id
93 schema:value pub.1027904064
94 rdf:type schema:PropertyValue
95 Ncde716e3eb48450dbe66a7eb1e82b735 rdf:first sg:person.01137464074.01
96 rdf:rest N5c9b1f3d939d4d63a3ebad2330649e38
97 Nd874dd8fd6384919915de42d6313d281 schema:name doi
98 schema:value 10.1007/s10453-012-9264-0
99 rdf:type schema:PropertyValue
100 Ndcd583cdc3294514aecd2303fb332013 schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 Ne22fd5e6006745fab6352047f00b10b4 rdf:first sg:person.01207666013.58
103 rdf:rest rdf:nil
104 Nebb80a05083749bc9e1be29ca9b1f9dd rdf:first sg:person.0645040234.36
105 rdf:rest N089d629bfcb8424e9c4abb83795f6c0d
106 Nedb578786a9b4763a77f8e4d80c2604d rdf:first sg:person.01113221153.00
107 rdf:rest Na3c0b99137f146108545b9499bf64f7d
108 Nedd6d63f790141eb86d02b298f703984 schema:name Institute of Physical Biology, Toplarniska 19, 1000, Ljubljana, Slovenia
109 rdf:type schema:Organization
110 Nf23bd6e136894b5cbcd1ccd04f7ba0ec rdf:first sg:person.01154200725.62
111 rdf:rest N96092b7c652649da932fc95a61308f7f
112 Nfe29e2bcd3ac475f8bc9c6c8eefe0035 rdf:first sg:person.0704675400.84
113 rdf:rest Ne22fd5e6006745fab6352047f00b10b4
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
118 schema:name Microbiology
119 rdf:type schema:DefinedTerm
120 sg:grant.3055441 http://pending.schema.org/fundedItem sg:pub.10.1007/s10453-012-9264-0
121 rdf:type schema:MonetaryGrant
122 sg:journal.1133886 schema:issn 0393-5965
123 1573-3025
124 schema:name Aerobiologia
125 rdf:type schema:Periodical
126 sg:person.01004257645.86 schema:affiliation https://www.grid.ac/institutes/grid.8591.5
127 schema:familyName Cattaneo
128 schema:givenName Arlette
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01004257645.86
130 rdf:type schema:Person
131 sg:person.01024316077.25 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
132 schema:familyName Davis-Richardson
133 schema:givenName Austin G.
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024316077.25
135 rdf:type schema:Person
136 sg:person.01027402034.57 schema:affiliation N10c1c544989246e1a84df60b0a9ca49c
137 schema:familyName Beck
138 schema:givenName Andreas
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027402034.57
140 rdf:type schema:Person
141 sg:person.01071350674.20 schema:affiliation N435c348f817748f39f62e0ad5c0ce930
142 schema:familyName Kennedy
143 schema:givenName Suzanne
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071350674.20
145 rdf:type schema:Person
146 sg:person.01072431277.90 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
147 schema:familyName Gano
148 schema:givenName Kelsey A.
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072431277.90
150 rdf:type schema:Person
151 sg:person.01113221153.00 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
152 schema:familyName Giongo
153 schema:givenName Adriana
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113221153.00
155 rdf:type schema:Person
156 sg:person.01137464074.01 schema:affiliation https://www.grid.ac/institutes/grid.8591.5
157 schema:familyName Aung
158 schema:givenName Yin-Yin
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137464074.01
160 rdf:type schema:Person
161 sg:person.01154200725.62 schema:affiliation Nedd6d63f790141eb86d02b298f703984
162 schema:familyName Lapanje
163 schema:givenName Ales
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154200725.62
165 rdf:type schema:Person
166 sg:person.01207666013.58 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
167 schema:familyName Triplett
168 schema:givenName Eric W.
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207666013.58
170 rdf:type schema:Person
171 sg:person.01227447553.36 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
172 schema:familyName Crabb
173 schema:givenName David B.
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227447553.36
175 rdf:type schema:Person
176 sg:person.01364017155.92 schema:affiliation https://www.grid.ac/institutes/grid.8591.5
177 schema:familyName Broughton
178 schema:givenName William J.
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364017155.92
180 rdf:type schema:Person
181 sg:person.01370141074.27 schema:affiliation https://www.grid.ac/institutes/grid.5560.6
182 schema:familyName Kort
183 schema:givenName Renate
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370141074.27
185 rdf:type schema:Person
186 sg:person.0575364545.05 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
187 schema:familyName Farmerie
188 schema:givenName William G.
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575364545.05
190 rdf:type schema:Person
191 sg:person.0576725034.96 schema:affiliation https://www.grid.ac/institutes/grid.5560.6
192 schema:familyName Brumsack
193 schema:givenName Hans-Jürgen
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576725034.96
195 rdf:type schema:Person
196 sg:person.0645040234.36 schema:affiliation https://www.grid.ac/institutes/grid.5560.6
197 schema:familyName Schnetger
198 schema:givenName Bernhard
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645040234.36
200 rdf:type schema:Person
201 sg:person.0704675400.84 schema:affiliation https://www.grid.ac/institutes/grid.14095.39
202 schema:familyName Gorbushina
203 schema:givenName Anna A.
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704675400.84
205 rdf:type schema:Person
206 sg:person.0707007074.06 schema:affiliation https://www.grid.ac/institutes/grid.8591.5
207 schema:familyName Favet
208 schema:givenName Jocelyne
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707007074.06
210 rdf:type schema:Person
211 sg:pub.10.1007/s002160050614 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051702407
212 https://doi.org/10.1007/s002160050614
213 rdf:type schema:CreativeWork
214 sg:pub.10.1007/s10453-010-9173-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1033578497
215 https://doi.org/10.1007/s10453-010-9173-z
216 rdf:type schema:CreativeWork
217 sg:pub.10.1007/s10453-011-9230-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036246667
218 https://doi.org/10.1007/s10453-011-9230-2
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/434816a schema:sameAs https://app.dimensions.ai/details/publication/pub.1040879530
221 https://doi.org/10.1038/434816a
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/ismej.2010.16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050994807
224 https://doi.org/10.1038/ismej.2010.16
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/ismej.2012.152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023561351
227 https://doi.org/10.1038/ismej.2012.152
228 rdf:type schema:CreativeWork
229 sg:pub.10.1038/nrmicro1160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031640134
230 https://doi.org/10.1038/nrmicro1160
231 rdf:type schema:CreativeWork
232 https://app.dimensions.ai/details/publication/pub.1080013201 schema:CreativeWork
233 https://doi.org/10.1016/0303-2647(78)90023-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005696076
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/j.aeolia.2011.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039743600
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/j.atmosenv.2007.01.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038551242
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/j.earscirev.2006.06.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006545737
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.envpol.2010.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045958676
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.ibiod.2003.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020343326
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.soilbio.2010.02.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049545348
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.tree.2006.07.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031629347
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/s0168-1656(99)00154-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038625209
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1042/bj1251075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026778829
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1093/nar/gkn879 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044918953
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1094/mpmi.1999.12.4.293 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060080067
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1101/gr.1390403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014635681
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1111/j.1440-1835.2010.00579.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009568861
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1111/j.1462-2920.2007.01461.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051265957
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1128/aem.01526-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014766903
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1128/cmr.00039-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034554429
266 rdf:type schema:CreativeWork
267 https://doi.org/10.3390/d2071015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000307020
268 rdf:type schema:CreativeWork
269 https://www.grid.ac/institutes/grid.14095.39 schema:alternateName Freie Universität Berlin
270 schema:name Abteilung Material und Umwelt, Bundesanstalt für Material-forschung, und -prüfung, Fabeckstrasse 62 A, 14195, Berlin, Germany
271 Fachbereich Biologie, Chemie und Pharmazie & Geowissenschaften, Freie Universität Berlin, Malteserstrasse 74-100, 12249, Berlin, Germany
272 Université de Genève, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland
273 rdf:type schema:Organization
274 https://www.grid.ac/institutes/grid.15276.37 schema:alternateName University of Florida
275 schema:name Department of Microbiology and Cell Science, University of Florida, Museum Road, 32611-0700, Gainesville, FL, USA
276 Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, 2033 Mowry Road, 32610, Gainesville, FL, USA
277 rdf:type schema:Organization
278 https://www.grid.ac/institutes/grid.5560.6 schema:alternateName Carl von Ossietzky University of Oldenburg
279 schema:name Elektronenmikroskopie, Carl von Ossietzky Universität, Oldenburg, Carl-von-Ossietzky str. 9-11, 26111, Oldenburg, Germany
280 Microbiogeochemie, ICBM, Carl von Ossietzky Universität, Oldenburg, Carl-von-Ossietzky str. 9-11, 26111, Oldenburg, Germany
281 rdf:type schema:Organization
282 https://www.grid.ac/institutes/grid.8591.5 schema:alternateName University of Geneva
283 schema:name Abteilung Material und Umwelt, Bundesanstalt für Material-forschung, und -prüfung, Fabeckstrasse 62 A, 14195, Berlin, Germany
284 Université de Genève, Sciences III, 30 quai Ernest-Ansermet, 1211, Geneve 4, Switzerland
285 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...