Oligosaccharide model of the vascular endothelial glycocalyx in physiological flow View Full Text


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Article Info

DATE

2018-02

AUTHORS

Maria Pikoula, Matthew B. Tessier, Robert J. Woods, Yiannis Ventikos

ABSTRACT

Experiments have consistently revealed the pivotal role of the endothelial glycocalyx layer in vasoregulation and the layer's contribution to mechanotransduction pathways. However, the exact mechanism by which the glycocalyx mediates fluid shear stress remains elusive. This study employs atomic-scale molecular simulations with the aim of investigating the conformational and orientation properties of highly flexible oligosaccharide components of the glycocalyx and their suitability as transduction molecules under hydrodynamic loading. Fluid flow was shown to have nearly no effect on the conformation populations explored by the oligosaccharide, in comparison with static (diffusion) conditions. However, the glycan exhibited a significant orientation change, when compared to simple diffusion, aligning itself with the flow direction. It is the tethered end of the glycan, an asparagine amino acid, which experienced conformational changes as a result of this flow-induced bias. Our results suggest that shear flow through the layer can have an impact on the conformational properties of saccharide-decorated transmembrane proteins, thus acting as a mechanosensor. More... »

PAGES

21

References to SciGraph publications

  • 2013-06. Heparan Sulfate Regrowth Profiles Under Laminar Shear Flow Following Enzymatic Degradation in CELLULAR AND MOLECULAR BIOENGINEERING
  • 2008-04. Effects of Disturbed Flow on Endothelial Cells in ANNALS OF BIOMEDICAL ENGINEERING
  • 2002-09. Molecular dynamics simulations of biomolecules in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2007-02. Emerging glycomics technologies in NATURE CHEMICAL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10404-018-2037-5

    DOI

    http://dx.doi.org/10.1007/s10404-018-2037-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1100691446

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29568255


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