A droplet microfluidic approach to single-stream nucleic acid isolation and mutation detection View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-08

AUTHORS

Dong Jin Shin, Yi Zhang, Tza-Huei Wang

ABSTRACT

In this work, a droplet microfluidic platform for genetic mutation detection from crude biosample is described. Single-stream integration of nucleic acid isolation and amplification is realized on a simple fluidic cartridge. Subsequent DNA melting curve is employed with signal normalizing algorithm to differentiate heterozygous K-ras codon 12 c.25G>A mutant from the wildtype. This technique showcases an alternative to modular bench-top approaches for genetic mutation screening, which is of interest to decentralized diagnostic platforms. More... »

PAGES

425-430

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10404-013-1305-7

DOI

http://dx.doi.org/10.1007/s10404-013-1305-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012838472

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25386112


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