Corrosion casts of big bubbles formed during deep anterior lamellar keratoplasty View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-07-19

AUTHORS

Sepehr Feizi, Mozhgan Rezaei Kanavi, Davood Kharaghani, Sahar Balagholi, Masoumeh Meskinfam, Mohammad Ali Javadi

ABSTRACT

PurposeTo characterize the walls of big bubbles formed during deep anterior lamellar keratoplasty (DALK) using the corrosion casting technique.MethodsFresh corneoscleral buttons with normal transparency and without any known eye diseases (n = 11) were obtained from 11 human donors. A 20-gauge needle was used to inject a solution of 20 % polyvinyl alcohol (PVA) immediately beneath the corneal endothelium to form big bubbles in eight corneoscleral buttons. In the second experiment on three corneoscleral buttons, a big bubble was first formed by air injection beneath the endothelium. Thereafter, 20 % PVA was injected into the bubble space. Scanning electron microscopy was used to characterize the surfaces of the casts, which replicated the walls of the big bubbles.ResultsA type-1 bubble was formed in all corneas. In one cornea, one type-1 bubble was initially formed centrally, and while it was enlarged, an eccentric type-2 bubble appeared. Scanning electron microscopy showed that the casts of type-1 bubbles had two distinct surfaces. The anterior surface demonstrated several holes or pits, depending on the material used for the bubble formation, whereas the posterior surface exhibited an uneven surface. The anterior and posterior surfaces of the type-2 cast were more or less similar. A communication measuring 531.9 µm in length and 171.4 µm in diameter was found between the two bubbles.ConclusionsThe corrosion casting technique provides a permanent three-dimensional record of the potential spaces and barriers in the posterior corneal stroma, which explains several features associated with big-bubble DALK. More... »

PAGES

492-499

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10384-016-0465-x

DOI

http://dx.doi.org/10.1007/s10384-016-0465-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040954231

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27435994


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