Experimental Design in Ion Chromatography: Effect of the Organic Modifier and Complexing Agent on the Retention of Alkaline and Alkaline ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-06

AUTHORS

Fabrizio Ruggieri, Angelo Antonio D’Archivio, Martina Foschi, Maria Anna Maggi

ABSTRACT

A Box–Behnken experimental design combined with response surface methodology was applied to investigate the effect of the eluent composition on the retention of common cations in ion chromatography. In particular, we modelled the retention factors of Na+, K+, NH4+, Ca++ and Mg++ and the chromatogram resolution. Response surfaces were evaluated to assess interactive effects of nitric acid, pyridine-2,6-dicarboxylic acid and percentage of acetonitrile in the eluent on the above parameters. Nitric acid concentration and acetonitrile content appeared as the most and the least influential components of the eluent in determining the retention factors of individual ions or the global resolution of the chromatogram, while the concentration of the complexing agent exhibits an intermediate role. As expected, acidification of the mobile phase produces a decrease in the retention factors and a progressive worsening of resolution, and this effect can be modulated by the concentration of the complexing agent. The proposed approach seems suitable to investigate the retention phenomena in ion chromatography under application of complex eluent phases. More... »

PAGES

853-860

Journal

TITLE

Chromatographia

ISSUE

6

VOLUME

80

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10337-017-3284-4

DOI

http://dx.doi.org/10.1007/s10337-017-3284-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084024403


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