Biological control of rice blast by the epiphytic bacterium Erwinia ananas transformed with a chitinolytic enzyme gene from an antagonistic ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-08

AUTHORS

Nobutaka Someya, Shinichi Numata, Masami Nakajima, Akira Hasebe, Tadaaki Hibi, Katsumi Akutsu

ABSTRACT

The gene chiA, encoding for the endochitinase ChiA, was cloned from Serratia marcescens strain B2, a tomato epiphytic bacterium, and introduced into the epiphytic bacterium Erwinia ananas NR-1, isolated from rice phylloplane. The gene chiA was introduced under the control of two types of promoter into a broad-host-range plasmid vector. The vector contained various fragments with promoter activity isolated from E. ananas chromosomal DNA. The constructed vectors were designated pchiA-V1pcf9 and pchiA-V1pcf53 for their respective promoters. E. ananas NR-1 transformed with either of these vectors produced and secreted ChiA. The antifungal activity of ChiA produced by transformed E. ananas NR-1 was demonstrated in vitro by the inhibition of Pyricularia oryzae germ tube elongation such as bursting of the hyphal tip. Transformed E. ananas NR-1 suppressed the incidence of rice blast caused by P. oryzae under greenhouse conditions; however, the magnitude of the suppressive effect depended on which promoter was used. Both transformants and the nontransformant E. ananas NR-1 survived on rice phylloplane. It is expected that the rice epiphytic bacterium E. ananas NR-1 carrying a chitinolytic enzyme gene is an efficient biological control agent against rice blast. More... »

PAGES

276-282

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10327-003-0043-1

DOI

http://dx.doi.org/10.1007/s10327-003-0043-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037316359


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410773.6", 
          "name": [
            "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Someya", 
        "givenName": "Nobutaka", 
        "id": "sg:person.01366220123.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366220123.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410773.6", 
          "name": [
            "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Numata", 
        "givenName": "Shinichi", 
        "id": "sg:person.013765507733.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013765507733.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410773.6", 
          "name": [
            "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakajima", 
        "givenName": "Masami", 
        "id": "sg:person.014556156227.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014556156227.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Agro-Environmental Sciences, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410826.9", 
          "name": [
            "National Institute for Agro-Environmental Sciences, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hasebe", 
        "givenName": "Akira", 
        "id": "sg:person.0643650266.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643650266.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hibi", 
        "givenName": "Tadaaki", 
        "id": "sg:person.015162370531.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015162370531.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410773.6", 
          "name": [
            "Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akutsu", 
        "givenName": "Katsumi", 
        "id": "sg:person.01070241277.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070241277.92"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2003-08", 
    "datePublishedReg": "2003-08-01", 
    "description": "The gene chiA, encoding for the endochitinase ChiA, was cloned from Serratia marcescens strain B2, a tomato epiphytic bacterium, and introduced into the epiphytic bacterium Erwinia ananas NR-1, isolated from rice phylloplane. The gene chiA was introduced under the control of two types of promoter into a broad-host-range plasmid vector. The vector contained various fragments with promoter activity isolated from E. ananas chromosomal DNA. The constructed vectors were designated pchiA-V1pcf9 and pchiA-V1pcf53 for their respective promoters. E. ananas NR-1 transformed with either of these vectors produced and secreted ChiA. The antifungal activity of ChiA produced by transformed E. ananas NR-1 was demonstrated in vitro by the inhibition of Pyricularia oryzae germ tube elongation such as bursting of the hyphal tip. Transformed E. ananas NR-1 suppressed the incidence of rice blast caused by P. oryzae under greenhouse conditions; however, the magnitude of the suppressive effect depended on which promoter was used. Both transformants and the nontransformant E. ananas NR-1 survived on rice phylloplane. It is expected that the rice epiphytic bacterium E. ananas NR-1 carrying a chitinolytic enzyme gene is an efficient biological control agent against rice blast.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s10327-003-0043-1", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1312372", 
        "issn": [
          "1345-2630", 
          "1610-739X"
        ], 
        "name": "Journal of General Plant Pathology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "69"
      }
    ], 
    "keywords": [
      "rice blast", 
      "range plasmid vector", 
      "rice phylloplane", 
      "NR-1", 
      "enzyme genes", 
      "efficient biological control agent", 
      "biological control agents", 
      "types of promoters", 
      "plasmid vector", 
      "biological control", 
      "epiphytic bacterium", 
      "germ tube elongation", 
      "chromosomal DNA", 
      "Serratia marcescens", 
      "hyphal tips", 
      "respective promoters", 
      "promoter activity", 
      "P. oryzae", 
      "control agents", 
      "greenhouse conditions", 
      "tube elongation", 
      "antagonistic bacterium", 
      "promoter", 
      "Erwinia ananas", 
      "phylloplane", 
      "genes", 
      "chia", 
      "bacterium", 
      "vector", 
      "antifungal activity", 
      "ChiA.", 
      "transformants", 
      "Erwinia", 
      "marcescens", 
      "oryzae", 
      "DNA", 
      "Ananas", 
      "blasts", 
      "suppressive effect", 
      "activity", 
      "fragments", 
      "elongation", 
      "inhibition", 
      "tip", 
      "B2", 
      "agents", 
      "control", 
      "e.", 
      "types", 
      "conditions", 
      "magnitude", 
      "effect", 
      "bursting", 
      "incidence"
    ], 
    "name": "Biological control of rice blast by the epiphytic bacterium Erwinia ananas transformed with a chitinolytic enzyme gene from an antagonistic bacterium, Serratia marcescens strain B2", 
    "pagination": "276-282", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037316359"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10327-003-0043-1"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10327-003-0043-1", 
      "https://app.dimensions.ai/details/publication/pub.1037316359"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-11-24T20:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_364.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s10327-003-0043-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10327-003-0043-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10327-003-0043-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10327-003-0043-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10327-003-0043-1'


 

This table displays all metadata directly associated to this object as RDF triples.

152 TRIPLES      20 PREDICATES      79 URIs      71 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10327-003-0043-1 schema:about anzsrc-for:06
2 anzsrc-for:0605
3 schema:author Nfe19b0ecc98742ffb265d6729f7e9b38
4 schema:datePublished 2003-08
5 schema:datePublishedReg 2003-08-01
6 schema:description The gene chiA, encoding for the endochitinase ChiA, was cloned from Serratia marcescens strain B2, a tomato epiphytic bacterium, and introduced into the epiphytic bacterium Erwinia ananas NR-1, isolated from rice phylloplane. The gene chiA was introduced under the control of two types of promoter into a broad-host-range plasmid vector. The vector contained various fragments with promoter activity isolated from E. ananas chromosomal DNA. The constructed vectors were designated pchiA-V1pcf9 and pchiA-V1pcf53 for their respective promoters. E. ananas NR-1 transformed with either of these vectors produced and secreted ChiA. The antifungal activity of ChiA produced by transformed E. ananas NR-1 was demonstrated in vitro by the inhibition of Pyricularia oryzae germ tube elongation such as bursting of the hyphal tip. Transformed E. ananas NR-1 suppressed the incidence of rice blast caused by P. oryzae under greenhouse conditions; however, the magnitude of the suppressive effect depended on which promoter was used. Both transformants and the nontransformant E. ananas NR-1 survived on rice phylloplane. It is expected that the rice epiphytic bacterium E. ananas NR-1 carrying a chitinolytic enzyme gene is an efficient biological control agent against rice blast.
7 schema:genre article
8 schema:isAccessibleForFree false
9 schema:isPartOf N5d6784ee3c5543b596a34c0c9a8a3eb6
10 N70ac68429c4a4e7e99ba696ac5c2339d
11 sg:journal.1312372
12 schema:keywords Ananas
13 B2
14 ChiA.
15 DNA
16 Erwinia
17 Erwinia ananas
18 NR-1
19 P. oryzae
20 Serratia marcescens
21 activity
22 agents
23 antagonistic bacterium
24 antifungal activity
25 bacterium
26 biological control
27 biological control agents
28 blasts
29 bursting
30 chia
31 chromosomal DNA
32 conditions
33 control
34 control agents
35 e.
36 effect
37 efficient biological control agent
38 elongation
39 enzyme genes
40 epiphytic bacterium
41 fragments
42 genes
43 germ tube elongation
44 greenhouse conditions
45 hyphal tips
46 incidence
47 inhibition
48 magnitude
49 marcescens
50 oryzae
51 phylloplane
52 plasmid vector
53 promoter
54 promoter activity
55 range plasmid vector
56 respective promoters
57 rice blast
58 rice phylloplane
59 suppressive effect
60 tip
61 transformants
62 tube elongation
63 types
64 types of promoters
65 vector
66 schema:name Biological control of rice blast by the epiphytic bacterium Erwinia ananas transformed with a chitinolytic enzyme gene from an antagonistic bacterium, Serratia marcescens strain B2
67 schema:pagination 276-282
68 schema:productId N9ccd0d9f6cbe490ea52605e644e3b77a
69 Ne5a791b9d2bc4ac881bfe470a5cd7d5d
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037316359
71 https://doi.org/10.1007/s10327-003-0043-1
72 schema:sdDatePublished 2022-11-24T20:50
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N9d9aebec1d8443e5910f4502bd14312e
75 schema:url https://doi.org/10.1007/s10327-003-0043-1
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N403a2eeb2b984cc7a8f4bb6e73f0112c rdf:first sg:person.013765507733.75
80 rdf:rest Nc47b24d6551e428a899d8ae90dfcf55c
81 N4d7aff62c4f7493baf1e4f465d4b6cf1 rdf:first sg:person.015162370531.59
82 rdf:rest Nc5efcfcb58644e06b4baac71d53ac812
83 N5d6784ee3c5543b596a34c0c9a8a3eb6 schema:issueNumber 4
84 rdf:type schema:PublicationIssue
85 N70ac68429c4a4e7e99ba696ac5c2339d schema:volumeNumber 69
86 rdf:type schema:PublicationVolume
87 N9ccd0d9f6cbe490ea52605e644e3b77a schema:name dimensions_id
88 schema:value pub.1037316359
89 rdf:type schema:PropertyValue
90 N9d9aebec1d8443e5910f4502bd14312e schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 Nc47b24d6551e428a899d8ae90dfcf55c rdf:first sg:person.014556156227.33
93 rdf:rest Nd9cd8329e8d845f4b9c911df5021da40
94 Nc5efcfcb58644e06b4baac71d53ac812 rdf:first sg:person.01070241277.92
95 rdf:rest rdf:nil
96 Nd9cd8329e8d845f4b9c911df5021da40 rdf:first sg:person.0643650266.82
97 rdf:rest N4d7aff62c4f7493baf1e4f465d4b6cf1
98 Ne5a791b9d2bc4ac881bfe470a5cd7d5d schema:name doi
99 schema:value 10.1007/s10327-003-0043-1
100 rdf:type schema:PropertyValue
101 Nfe19b0ecc98742ffb265d6729f7e9b38 rdf:first sg:person.01366220123.61
102 rdf:rest N403a2eeb2b984cc7a8f4bb6e73f0112c
103 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
104 schema:name Biological Sciences
105 rdf:type schema:DefinedTerm
106 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
107 schema:name Microbiology
108 rdf:type schema:DefinedTerm
109 sg:journal.1312372 schema:issn 1345-2630
110 1610-739X
111 schema:name Journal of General Plant Pathology
112 schema:publisher Springer Nature
113 rdf:type schema:Periodical
114 sg:person.01070241277.92 schema:affiliation grid-institutes:grid.410773.6
115 schema:familyName Akutsu
116 schema:givenName Katsumi
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070241277.92
118 rdf:type schema:Person
119 sg:person.01366220123.61 schema:affiliation grid-institutes:grid.410773.6
120 schema:familyName Someya
121 schema:givenName Nobutaka
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366220123.61
123 rdf:type schema:Person
124 sg:person.013765507733.75 schema:affiliation grid-institutes:grid.410773.6
125 schema:familyName Numata
126 schema:givenName Shinichi
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013765507733.75
128 rdf:type schema:Person
129 sg:person.014556156227.33 schema:affiliation grid-institutes:grid.410773.6
130 schema:familyName Nakajima
131 schema:givenName Masami
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014556156227.33
133 rdf:type schema:Person
134 sg:person.015162370531.59 schema:affiliation grid-institutes:grid.26999.3d
135 schema:familyName Hibi
136 schema:givenName Tadaaki
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015162370531.59
138 rdf:type schema:Person
139 sg:person.0643650266.82 schema:affiliation grid-institutes:grid.410826.9
140 schema:familyName Hasebe
141 schema:givenName Akira
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643650266.82
143 rdf:type schema:Person
144 grid-institutes:grid.26999.3d schema:alternateName Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan
145 schema:name Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan
146 rdf:type schema:Organization
147 grid-institutes:grid.410773.6 schema:alternateName Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan
148 schema:name Faculty of Agriculture, Ibaraki University, 300-0393, Ami-machi, Ibaraki, Japan
149 rdf:type schema:Organization
150 grid-institutes:grid.410826.9 schema:alternateName National Institute for Agro-Environmental Sciences, Tsukuba, Japan
151 schema:name National Institute for Agro-Environmental Sciences, Tsukuba, Japan
152 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...