Effect of dissolved oxygen on l-methionine production from glycerol by Escherichia coli W3110BL using metabolic flux analysis method View Full Text


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Article Info

DATE

2020-03-01

AUTHORS

Kun Niu, Yue-Ying Xu, Wang-Jie Wu, Hai-Yan Zhou, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

l-Methionine is an essential amino acid in humans, which plays an important role in the synthesis of some important amino acids and proteins. In this work, metabolic flux of batch fermentation of l-methionine with recombinant Escherichia coli W3110BL was analyzed using the flux balance analysis method, which estimated the intracellular flux distributions under different dissolved oxygen conditions. The results revealed the producing l-methionine flux of 4.8 mmol/(g cell·h) [based on the glycerol uptake flux of 100 mmol/(g cell·h)] was obtained at 30% dissolved oxygen level which was higher than that of other dissolved oxygen levels. The carbon fluxes for synthesizing l-methionine were mainly obtained from the pathway of phosphoenolpyruvate to oxaloacetic acid [15.6 mmol/(g cell·h)] but not from the TCA cycle. Hence, increasing the flow from phosphoenolpyruvate to oxaloacetic acid by enhancing the enzyme activity of phosphoenolpyruvate carboxylase might be conducive to the production of l-methionine. Additionally, pentose phosphate pathway could provide a large amount of reducing power NADPH for the synthesis of amino acids and the flux could increase from 41 mmol/(g cell·h) to 51 mmol/(g cell·h) when changing the dissolved oxygen levels, thus meeting the requirement of NADPH for l-methionine production and biomass synthesis. Therefore, the following modification of the strains should based on the improvement of the key pathway and the NAD(P)/NAD(P)H metabolism. More... »

PAGES

287-297

References to SciGraph publications

  • 2011-02-16. Diversity of metabolic shift in response to oxygen deprivation in Corynebacterium glutamicum and its close relatives in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2013. Encyclopedia of Systems Biology in NONE
  • 2019-01-02. High-yield production of l-serine from glycerol by engineered Escherichia coli in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2008-10-25. Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli in CURRENT MICROBIOLOGY
  • 2018-07-14. Two-stage oxygen supply strategy based on energy metabolism analysis for improving acetic acid production by Acetobacter pasteurianus in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2013-10-19. Flux balance analysis of Chlorella sp. FC2 IITG under photoautotrophic and heterotrophic growth conditions in PHOTOSYNTHESIS RESEARCH
  • 2017-01-10. Recent advances in biotechnological applications of alcohol dehydrogenases in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2018-01-22. Metabolic flux analysis and the NAD(P)H/NAD(P)+ ratios in chemostat cultures of Azotobacter vinelandii in MICROBIAL CELL FACTORIES
  • 2015-04. Identifying a gene knockout strategy using a hybrid of the bat algorithm and flux balance analysis to enhance the production of succinate and lactate in Escherichia coli in BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
  • 2017-07-14. Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations in BIOTECHNOLOGY FOR BIOFUELS
  • 2009-05-21. 13C-based metabolic flux analysis in NATURE PROTOCOLS
  • 2013-07-19. Expression of NAD(H) Kinase and Glucose-6-Phosphate Dehydrogenase Improve NADPH Supply and l-isoleucine Biosynthesis in Corynebacterium glutamicum ssp. lactofermentum in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2018-07-09. Metabolic engineering of E. coli for the production of O-succinyl-l-homoserine with high yield in 3 BIOTECH
  • 2016-11-14. Metabolic engineering of Escherichia coli W3110 for the production of l-methionine in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2014-11-08. Methionine production—a critical review in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2019-02-19. Enhanced L-methionine production by genetically engineered Escherichia coli through fermentation optimization in 3 BIOTECH
  • 2014-02. Enhancing the carbon flux and NADPH supply to increase L-isoleucine production in Corynebacterium glutamicum in BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
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    34 schema:description Abstractl-Methionine is an essential amino acid in humans, which plays an important role in the synthesis of some important amino acids and proteins. In this work, metabolic flux of batch fermentation of l-methionine with recombinant Escherichia coli W3110BL was analyzed using the flux balance analysis method, which estimated the intracellular flux distributions under different dissolved oxygen conditions. The results revealed the producing l-methionine flux of 4.8 mmol/(g cell·h) [based on the glycerol uptake flux of 100 mmol/(g cell·h)] was obtained at 30% dissolved oxygen level which was higher than that of other dissolved oxygen levels. The carbon fluxes for synthesizing l-methionine were mainly obtained from the pathway of phosphoenolpyruvate to oxaloacetic acid [15.6 mmol/(g cell·h)] but not from the TCA cycle. Hence, increasing the flow from phosphoenolpyruvate to oxaloacetic acid by enhancing the enzyme activity of phosphoenolpyruvate carboxylase might be conducive to the production of l-methionine. Additionally, pentose phosphate pathway could provide a large amount of reducing power NADPH for the synthesis of amino acids and the flux could increase from 41 mmol/(g cell·h) to 51 mmol/(g cell·h) when changing the dissolved oxygen levels, thus meeting the requirement of NADPH for l-methionine production and biomass synthesis. Therefore, the following modification of the strains should based on the improvement of the key pathway and the NAD(P)/NAD(P)H metabolism.
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    42 Escherichia coli W3110BL
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