Improved l-ornithine production in Corynebacterium crenatum by introducing an artificial linear transacetylation pathway View Full Text


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Article Info

DATE

2018-06

AUTHORS

Qunfeng Shu, Meijuan Xu, Jing Li, Taowei Yang, Xian Zhang, Zhenghong Xu, Zhiming Rao

ABSTRACT

L-Ornithine is a non-protein amino acid with extensive applications in the food and pharmaceutical industries. In this study, we performed metabolic pathway engineering of an L-arginine hyper-producing strain of Corynebacterium crenatum for L-ornithine production. First, we amplified the L-ornithine biosynthetic pathway flux by blocking the competing branch of the pathway. To enhance L-ornithine synthesis, we performed site-directed mutagenesis of the ornithine-binding sites to solve the problem of L-ornithine feedback inhibition for ornithine acetyltransferase. Alternatively, the genes argA from Escherichia coli and argE from Serratia marcescens, encoding the enzymes N-acetyl glutamate synthase and N-acetyl-L-ornithine deacetylase, respectively, were introduced into Corynebacterium crenatum to mimic the linear pathway of L-ornithine biosynthesis. Fermentation of the resulting strain in a 5-L bioreactor allowed a dramatically increased production of L-ornithine, 40.4 g/L, with an overall productivity of 0.673 g/L/h over 60 h. This demonstrates that an increased level of transacetylation is beneficial for L-ornithine biosynthesis. More... »

PAGES

393-404

References to SciGraph publications

  • 2017-02. Reengineering of the feedback-inhibition enzyme N-acetyl-l-glutamate kinase to enhance l-arginine production in Corynebacterium crenatum in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2013-10. Metabolic engineering of Corynebacterium glutamicum for increasing the production of l-ornithine by increasing NADPH availability in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2014-02. Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2013-12. Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine in BMC BIOTECHNOLOGY
  • 2015-12. Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine in NATURE COMMUNICATIONS
  • 2016-01. Controlling the transcription levels of argGH redistributed l-arginine metabolic flux in N-acetylglutamate kinase and ArgR-deregulated Corynebacterium crenatum in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2002-11. Efficient Electrotransformation of Corynebacterium diphtheriae with a Mini-Replicon Derived from the Corynebacterium glutamicum Plasmid pGA1 in CURRENT MICROBIOLOGY
  • 1983-07. Increased L-ornithine production by an arg mutant of Acinetobacter lwoffi in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2016-09. Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production in SCIENTIFIC REPORTS
  • 2017-12. Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production in MICROBIAL CELL FACTORIES
  • 2013-07. Characterization of 582 natural and synthetic terminators and quantification of their design constraints in NATURE METHODS
  • 2013-06. The Role of ARGR Repressor Regulation on L-arginine Production in Corynebacterium crenatum in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2006-06. Tricine–SDS-PAGE in NATURE PROTOCOLS
  • 2006-11. Genetic manipulation of a primary metabolic pathway for l-ornithine production in Escherichia coli in BIOTECHNOLOGY LETTERS
  • 2015-12. Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum in MICROBIAL CELL FACTORIES
  • 2013-04. Precise and reliable gene expression via standard transcription and translation initiation elements in NATURE METHODS
  • 2002-10. Molecular Evolution of the Lysine Biosynthetic Pathways in JOURNAL OF MOLECULAR EVOLUTION
  • 2009-10. Automated design of synthetic ribosome binding sites to control protein expression in NATURE BIOTECHNOLOGY
  • 2013-12. Identification of a Histidine Metal Ligand in the argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli in SPRINGERPLUS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10295-018-2037-1

    DOI

    http://dx.doi.org/10.1007/s10295-018-2037-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1103812050

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29728854


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