An integrated workflow for phenazine-modifying enzyme characterization View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-07

AUTHORS

R. Cameron Coates, Benjamin P. Bowen, Ernst Oberortner, Linda Thomashow, Michalis Hadjithomas, Zhiying Zhao, Jing Ke, Leslie Silva, Katherine Louie, Gaoyan Wang, David Robinson, Angela Tarver, Matthew Hamilton, Andrea Lubbe, Meghan Feltcher, Jeffery L. Dangl, Amrita Pati, David Weller, Trent R. Northen, Jan-Fang Cheng, Nigel J. Mouncey, Samuel Deutsch, Yasuo Yoshikuni

ABSTRACT

Increasing availability of new genomes and putative biosynthetic gene clusters (BGCs) has extended the opportunity to access novel chemical diversity for agriculture, medicine, environmental and industrial purposes. However, functional characterization of BGCs through heterologous expression is limited because expression may require complex regulatory mechanisms, specific folding or activation. We developed an integrated workflow for BGC characterization that integrates pathway identification, modular design, DNA synthesis, assembly and characterization. This workflow was applied to characterize multiple phenazine-modifying enzymes. Phenazine pathways are useful for this workflow because all phenazines are derived from a core scaffold for modification by diverse modifying enzymes (PhzM, PhzS, PhzH, and PhzO) that produce characterized compounds. We expressed refactored synthetic modules of previously uncharacterized phenazine BGCs heterologously in Escherichia coli and were able to identify metabolic intermediates they produced, including a previously unidentified metabolite. These results demonstrate how this approach can accelerate functional characterization of BGCs. More... »

PAGES

567-577

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-018-2025-5

DOI

http://dx.doi.org/10.1007/s10295-018-2025-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101546332

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29546662


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Coates", 
        "givenName": "R. Cameron", 
        "id": "sg:person.0732603767.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732603767.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lawrence Berkeley National Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.184769.5", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA", 
            "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bowen", 
        "givenName": "Benjamin P.", 
        "id": "sg:person.014566571137.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014566571137.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oberortner", 
        "givenName": "Ernst", 
        "id": "sg:person.01175117242.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175117242.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Washington State University", 
          "id": "https://www.grid.ac/institutes/grid.30064.31", 
          "name": [
            "USDA Agricultural Research Service, Wheat Health, Genetics and Quality, Washington State University, Pullman, WA, USA", 
            "Department of Plant Pathology, Washington State University, Pullman, WA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thomashow", 
        "givenName": "Linda", 
        "id": "sg:person.01140147531.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140147531.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hadjithomas", 
        "givenName": "Michalis", 
        "id": "sg:person.01276427560.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276427560.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhao", 
        "givenName": "Zhiying", 
        "id": "sg:person.01037171745.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037171745.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ke", 
        "givenName": "Jing", 
        "id": "sg:person.015031152411.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015031152411.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Silva", 
        "givenName": "Leslie", 
        "id": "sg:person.01074264157.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074264157.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Louie", 
        "givenName": "Katherine", 
        "id": "sg:person.01063033426.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063033426.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Gaoyan", 
        "id": "sg:person.013032330413.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013032330413.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Robinson", 
        "givenName": "David", 
        "id": "sg:person.010132775321.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010132775321.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tarver", 
        "givenName": "Angela", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hamilton", 
        "givenName": "Matthew", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lawrence Berkeley National Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.184769.5", 
          "name": [
            "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lubbe", 
        "givenName": "Andrea", 
        "id": "sg:person.016073642773.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016073642773.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of North Carolina at Chapel Hill", 
          "id": "https://www.grid.ac/institutes/grid.10698.36", 
          "name": [
            "Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Feltcher", 
        "givenName": "Meghan", 
        "id": "sg:person.0761117604.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761117604.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of North Carolina at Chapel Hill", 
          "id": "https://www.grid.ac/institutes/grid.10698.36", 
          "name": [
            "Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA", 
            "Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA", 
            "Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA", 
            "Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA", 
            "Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dangl", 
        "givenName": "Jeffery L.", 
        "id": "sg:person.015727674137.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015727674137.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pati", 
        "givenName": "Amrita", 
        "id": "sg:person.01104755463.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104755463.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Washington State University", 
          "id": "https://www.grid.ac/institutes/grid.30064.31", 
          "name": [
            "USDA Agricultural Research Service, Wheat Health, Genetics and Quality, Washington State University, Pullman, WA, USA", 
            "Department of Plant Pathology, Washington State University, Pullman, WA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weller", 
        "givenName": "David", 
        "id": "sg:person.01206262731.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206262731.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lawrence Berkeley National Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.184769.5", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA", 
            "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Northen", 
        "givenName": "Trent R.", 
        "id": "sg:person.01076170224.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076170224.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lawrence Berkeley National Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.184769.5", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA", 
            "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheng", 
        "givenName": "Jan-Fang", 
        "id": "sg:person.012533550217.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012533550217.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joint Genome Institute", 
          "id": "https://www.grid.ac/institutes/grid.451309.a", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mouncey", 
        "givenName": "Nigel J.", 
        "id": "sg:person.01144422167.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144422167.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lawrence Berkeley National Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.184769.5", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA", 
            "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA", 
            "Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Deutsch", 
        "givenName": "Samuel", 
        "id": "sg:person.01265042244.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265042244.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lawrence Berkeley National Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.184769.5", 
          "name": [
            "US DOE Joint Genome Institute, Walnut Creek, CA, USA", 
            "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA", 
            "Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoshikuni", 
        "givenName": "Yasuo", 
        "id": "sg:person.0714310565.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714310565.84"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.1222159110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000743942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.183.1.318-327.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002145874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.9.4034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002873189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro.2015.24", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005279381", 
          "https://doi.org/10.1038/nrmicro.2015.24"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2014.084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010581475", 
          "https://doi.org/10.1038/nprot.2014.084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/sb2000116", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011545005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms8832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012104624", 
          "https://doi.org/10.1038/ncomms8832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.phyto.44.013106.145710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013154308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02009-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020443656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/mmi.12566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023205996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/jr9370001704", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024139593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chembiol.2012.07.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025566658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.183.21.6454-6465.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034214640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.120163297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039276805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-40573-0_7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039872915", 
          "https://doi.org/10.1007/978-3-642-40573-0_7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1318", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040405189", 
          "https://doi.org/10.1038/nmeth.1318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1318", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040405189", 
          "https://doi.org/10.1038/nmeth.1318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-40573-0_10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040974317", 
          "https://doi.org/10.1007/978-3-642-40573-0_10"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hlca.192400701122", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048519528"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mbio.00932-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049896070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2014.08.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050106796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acssynbio.6b00200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055140892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi-5-330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060079261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi.2001.14.8.1006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060080440"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077070669", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083255199", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2017.02.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084068245"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-07", 
    "datePublishedReg": "2018-07-01", 
    "description": "Increasing availability of new genomes and putative biosynthetic gene clusters (BGCs) has extended the opportunity to access novel chemical diversity for agriculture, medicine, environmental and industrial purposes. However, functional characterization of BGCs through heterologous expression is limited because expression may require complex regulatory mechanisms, specific folding or activation. We developed an integrated workflow for BGC characterization that integrates pathway identification, modular design, DNA synthesis, assembly and characterization. This workflow was applied to characterize multiple phenazine-modifying enzymes. Phenazine pathways are useful for this workflow because all phenazines are derived from a core scaffold for modification by diverse modifying enzymes (PhzM, PhzS, PhzH, and PhzO) that produce characterized compounds. We expressed refactored synthetic modules of previously uncharacterized phenazine BGCs heterologously in Escherichia coli and were able to identify metabolic intermediates they produced, including a previously unidentified metabolite. These results demonstrate how this approach can accelerate functional characterization of BGCs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10295-018-2025-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1096599", 
        "issn": [
          "1367-5435", 
          "1476-5535"
        ], 
        "name": "Journal of Industrial Microbiology & Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "45"
      }
    ], 
    "name": "An integrated workflow for phenazine-modifying enzyme characterization", 
    "pagination": "567-577", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c4e75c5d97ea950970ce1790ff3749bebbc279826e49091dbe35b4bc5bad5daa"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29546662"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9705544"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10295-018-2025-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1101546332"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10295-018-2025-5", 
      "https://app.dimensions.ai/details/publication/pub.1101546332"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29207_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs10295-018-2025-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10295-018-2025-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10295-018-2025-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-018-2025-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-018-2025-5'


 

This table displays all metadata directly associated to this object as RDF triples.

319 TRIPLES      21 PREDICATES      55 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10295-018-2025-5 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Nf51d7950f5da4569adecbf07dd9f6876
4 schema:citation sg:pub.10.1007/978-3-642-40573-0_10
5 sg:pub.10.1007/978-3-642-40573-0_7
6 sg:pub.10.1038/ncomms8832
7 sg:pub.10.1038/nmeth.1318
8 sg:pub.10.1038/nprot.2014.084
9 sg:pub.10.1038/nrmicro.2015.24
10 https://app.dimensions.ai/details/publication/pub.1077070669
11 https://app.dimensions.ai/details/publication/pub.1083255199
12 https://doi.org/10.1002/hlca.192400701122
13 https://doi.org/10.1016/j.chembiol.2012.07.025
14 https://doi.org/10.1016/j.copbio.2017.02.008
15 https://doi.org/10.1016/j.sbi.2014.08.013
16 https://doi.org/10.1021/acssynbio.6b00200
17 https://doi.org/10.1021/sb2000116
18 https://doi.org/10.1039/jr9370001704
19 https://doi.org/10.1073/pnas.120163297
20 https://doi.org/10.1073/pnas.1222159110
21 https://doi.org/10.1073/pnas.91.9.4034
22 https://doi.org/10.1094/mpmi-5-330
23 https://doi.org/10.1094/mpmi.2001.14.8.1006
24 https://doi.org/10.1111/mmi.12566
25 https://doi.org/10.1128/aem.02009-09
26 https://doi.org/10.1128/jb.183.1.318-327.2001
27 https://doi.org/10.1128/jb.183.21.6454-6465.2001
28 https://doi.org/10.1128/mbio.00932-15
29 https://doi.org/10.1146/annurev.phyto.44.013106.145710
30 schema:datePublished 2018-07
31 schema:datePublishedReg 2018-07-01
32 schema:description Increasing availability of new genomes and putative biosynthetic gene clusters (BGCs) has extended the opportunity to access novel chemical diversity for agriculture, medicine, environmental and industrial purposes. However, functional characterization of BGCs through heterologous expression is limited because expression may require complex regulatory mechanisms, specific folding or activation. We developed an integrated workflow for BGC characterization that integrates pathway identification, modular design, DNA synthesis, assembly and characterization. This workflow was applied to characterize multiple phenazine-modifying enzymes. Phenazine pathways are useful for this workflow because all phenazines are derived from a core scaffold for modification by diverse modifying enzymes (PhzM, PhzS, PhzH, and PhzO) that produce characterized compounds. We expressed refactored synthetic modules of previously uncharacterized phenazine BGCs heterologously in Escherichia coli and were able to identify metabolic intermediates they produced, including a previously unidentified metabolite. These results demonstrate how this approach can accelerate functional characterization of BGCs.
33 schema:genre research_article
34 schema:inLanguage en
35 schema:isAccessibleForFree true
36 schema:isPartOf N3fb2d0f97ea5421d8aff26d896fb0269
37 N70f944e2569d47b689443ff293b37583
38 sg:journal.1096599
39 schema:name An integrated workflow for phenazine-modifying enzyme characterization
40 schema:pagination 567-577
41 schema:productId N0bebe3dedd234560806dc63b09b88f7f
42 N8c444cc2f7eb4fd1845e4a79d11d199a
43 Ncabbe4454ee84e1b8e000ceb660e7061
44 Ndc11cdd37b6c44959334323ee9fb7d5c
45 Nf3555c6ec1f74079917d8a6787e4812a
46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101546332
47 https://doi.org/10.1007/s10295-018-2025-5
48 schema:sdDatePublished 2019-04-11T11:55
49 schema:sdLicense https://scigraph.springernature.com/explorer/license/
50 schema:sdPublisher N49dabbf215e84aa58e855172ac4761e5
51 schema:url https://link.springer.com/10.1007%2Fs10295-018-2025-5
52 sgo:license sg:explorer/license/
53 sgo:sdDataset articles
54 rdf:type schema:ScholarlyArticle
55 N04542f614321439386dc2a0820dac176 rdf:first sg:person.016073642773.55
56 rdf:rest Na938a58e42bf4da3aebdaf4ff31036fb
57 N0481bf1ef353465d96857a65524cefac rdf:first Nb62e3d22bea040539f14f10b7301d15b
58 rdf:rest Nad924e5d438e46cca17b49ff282b87d9
59 N0971964e76394c51865dcc473fbcb84f schema:affiliation https://www.grid.ac/institutes/grid.451309.a
60 schema:familyName Hamilton
61 schema:givenName Matthew
62 rdf:type schema:Person
63 N0bebe3dedd234560806dc63b09b88f7f schema:name nlm_unique_id
64 schema:value 9705544
65 rdf:type schema:PropertyValue
66 N0dc785ce3d2f45e5b76e00629af04019 rdf:first sg:person.01140147531.37
67 rdf:rest N730dc1caf9f94754a6361d89ffd95cee
68 N1cdba6b1dbea4c0c9a53b259b0923083 rdf:first sg:person.010132775321.64
69 rdf:rest N0481bf1ef353465d96857a65524cefac
70 N36ff44e9da6c41cba863b85d58c41fa1 rdf:first sg:person.012533550217.36
71 rdf:rest N8b140c9829274b57918eeec78cc5553f
72 N3fb2d0f97ea5421d8aff26d896fb0269 schema:issueNumber 7
73 rdf:type schema:PublicationIssue
74 N49dabbf215e84aa58e855172ac4761e5 schema:name Springer Nature - SN SciGraph project
75 rdf:type schema:Organization
76 N55b6c6a80e804cb0ab4fb600fd89467e rdf:first sg:person.01265042244.14
77 rdf:rest Nbd4ac933c3ea494a899b151bc90a5b56
78 N62e17b837e8c4153a0fa529f51854b7e rdf:first sg:person.01104755463.41
79 rdf:rest Nfb7fd06d5c0b4c7393ca8dcbea8a627f
80 N70f944e2569d47b689443ff293b37583 schema:volumeNumber 45
81 rdf:type schema:PublicationVolume
82 N730dc1caf9f94754a6361d89ffd95cee rdf:first sg:person.01276427560.04
83 rdf:rest Nbdbaef2a63194d608254c2966dd64ac8
84 N796c941af9ee4e4fa1598b82bfc8cb8d rdf:first sg:person.01074264157.06
85 rdf:rest Nb29c2f4806a540a4b19b2fab06dae168
86 N8b140c9829274b57918eeec78cc5553f rdf:first sg:person.01144422167.54
87 rdf:rest N55b6c6a80e804cb0ab4fb600fd89467e
88 N8c444cc2f7eb4fd1845e4a79d11d199a schema:name pubmed_id
89 schema:value 29546662
90 rdf:type schema:PropertyValue
91 N9d250c80272542e79dec641b88738e5f rdf:first sg:person.014566571137.39
92 rdf:rest Nd56b0926639845398904322a5949f472
93 Na938a58e42bf4da3aebdaf4ff31036fb rdf:first sg:person.0761117604.03
94 rdf:rest Nd03edc6385954396ac27563c76a35763
95 Nad924e5d438e46cca17b49ff282b87d9 rdf:first N0971964e76394c51865dcc473fbcb84f
96 rdf:rest N04542f614321439386dc2a0820dac176
97 Naec10129fcee4c94867a3a12f6687fef rdf:first sg:person.01076170224.01
98 rdf:rest N36ff44e9da6c41cba863b85d58c41fa1
99 Nb29c2f4806a540a4b19b2fab06dae168 rdf:first sg:person.01063033426.28
100 rdf:rest Ncc1d40c6057944238dfb930bc78ea786
101 Nb62e3d22bea040539f14f10b7301d15b schema:affiliation https://www.grid.ac/institutes/grid.451309.a
102 schema:familyName Tarver
103 schema:givenName Angela
104 rdf:type schema:Person
105 Nbd4ac933c3ea494a899b151bc90a5b56 rdf:first sg:person.0714310565.84
106 rdf:rest rdf:nil
107 Nbdbaef2a63194d608254c2966dd64ac8 rdf:first sg:person.01037171745.50
108 rdf:rest Nc1e97d1538b64ca3875b767c61e2af0e
109 Nc1e97d1538b64ca3875b767c61e2af0e rdf:first sg:person.015031152411.61
110 rdf:rest N796c941af9ee4e4fa1598b82bfc8cb8d
111 Ncabbe4454ee84e1b8e000ceb660e7061 schema:name dimensions_id
112 schema:value pub.1101546332
113 rdf:type schema:PropertyValue
114 Ncc1d40c6057944238dfb930bc78ea786 rdf:first sg:person.013032330413.47
115 rdf:rest N1cdba6b1dbea4c0c9a53b259b0923083
116 Nd03edc6385954396ac27563c76a35763 rdf:first sg:person.015727674137.17
117 rdf:rest N62e17b837e8c4153a0fa529f51854b7e
118 Nd56b0926639845398904322a5949f472 rdf:first sg:person.01175117242.17
119 rdf:rest N0dc785ce3d2f45e5b76e00629af04019
120 Ndc11cdd37b6c44959334323ee9fb7d5c schema:name doi
121 schema:value 10.1007/s10295-018-2025-5
122 rdf:type schema:PropertyValue
123 Nf3555c6ec1f74079917d8a6787e4812a schema:name readcube_id
124 schema:value c4e75c5d97ea950970ce1790ff3749bebbc279826e49091dbe35b4bc5bad5daa
125 rdf:type schema:PropertyValue
126 Nf51d7950f5da4569adecbf07dd9f6876 rdf:first sg:person.0732603767.71
127 rdf:rest N9d250c80272542e79dec641b88738e5f
128 Nfb7fd06d5c0b4c7393ca8dcbea8a627f rdf:first sg:person.01206262731.42
129 rdf:rest Naec10129fcee4c94867a3a12f6687fef
130 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
131 schema:name Biological Sciences
132 rdf:type schema:DefinedTerm
133 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
134 schema:name Biochemistry and Cell Biology
135 rdf:type schema:DefinedTerm
136 sg:journal.1096599 schema:issn 1367-5435
137 1476-5535
138 schema:name Journal of Industrial Microbiology & Biotechnology
139 rdf:type schema:Periodical
140 sg:person.010132775321.64 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
141 schema:familyName Robinson
142 schema:givenName David
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010132775321.64
144 rdf:type schema:Person
145 sg:person.01037171745.50 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
146 schema:familyName Zhao
147 schema:givenName Zhiying
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037171745.50
149 rdf:type schema:Person
150 sg:person.01063033426.28 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
151 schema:familyName Louie
152 schema:givenName Katherine
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063033426.28
154 rdf:type schema:Person
155 sg:person.01074264157.06 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
156 schema:familyName Silva
157 schema:givenName Leslie
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074264157.06
159 rdf:type schema:Person
160 sg:person.01076170224.01 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
161 schema:familyName Northen
162 schema:givenName Trent R.
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076170224.01
164 rdf:type schema:Person
165 sg:person.01104755463.41 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
166 schema:familyName Pati
167 schema:givenName Amrita
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104755463.41
169 rdf:type schema:Person
170 sg:person.01140147531.37 schema:affiliation https://www.grid.ac/institutes/grid.30064.31
171 schema:familyName Thomashow
172 schema:givenName Linda
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140147531.37
174 rdf:type schema:Person
175 sg:person.01144422167.54 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
176 schema:familyName Mouncey
177 schema:givenName Nigel J.
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144422167.54
179 rdf:type schema:Person
180 sg:person.01175117242.17 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
181 schema:familyName Oberortner
182 schema:givenName Ernst
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175117242.17
184 rdf:type schema:Person
185 sg:person.01206262731.42 schema:affiliation https://www.grid.ac/institutes/grid.30064.31
186 schema:familyName Weller
187 schema:givenName David
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206262731.42
189 rdf:type schema:Person
190 sg:person.012533550217.36 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
191 schema:familyName Cheng
192 schema:givenName Jan-Fang
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012533550217.36
194 rdf:type schema:Person
195 sg:person.01265042244.14 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
196 schema:familyName Deutsch
197 schema:givenName Samuel
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265042244.14
199 rdf:type schema:Person
200 sg:person.01276427560.04 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
201 schema:familyName Hadjithomas
202 schema:givenName Michalis
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276427560.04
204 rdf:type schema:Person
205 sg:person.013032330413.47 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
206 schema:familyName Wang
207 schema:givenName Gaoyan
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013032330413.47
209 rdf:type schema:Person
210 sg:person.014566571137.39 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
211 schema:familyName Bowen
212 schema:givenName Benjamin P.
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014566571137.39
214 rdf:type schema:Person
215 sg:person.015031152411.61 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
216 schema:familyName Ke
217 schema:givenName Jing
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015031152411.61
219 rdf:type schema:Person
220 sg:person.015727674137.17 schema:affiliation https://www.grid.ac/institutes/grid.10698.36
221 schema:familyName Dangl
222 schema:givenName Jeffery L.
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015727674137.17
224 rdf:type schema:Person
225 sg:person.016073642773.55 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
226 schema:familyName Lubbe
227 schema:givenName Andrea
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016073642773.55
229 rdf:type schema:Person
230 sg:person.0714310565.84 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
231 schema:familyName Yoshikuni
232 schema:givenName Yasuo
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714310565.84
234 rdf:type schema:Person
235 sg:person.0732603767.71 schema:affiliation https://www.grid.ac/institutes/grid.451309.a
236 schema:familyName Coates
237 schema:givenName R. Cameron
238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732603767.71
239 rdf:type schema:Person
240 sg:person.0761117604.03 schema:affiliation https://www.grid.ac/institutes/grid.10698.36
241 schema:familyName Feltcher
242 schema:givenName Meghan
243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761117604.03
244 rdf:type schema:Person
245 sg:pub.10.1007/978-3-642-40573-0_10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040974317
246 https://doi.org/10.1007/978-3-642-40573-0_10
247 rdf:type schema:CreativeWork
248 sg:pub.10.1007/978-3-642-40573-0_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039872915
249 https://doi.org/10.1007/978-3-642-40573-0_7
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/ncomms8832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012104624
252 https://doi.org/10.1038/ncomms8832
253 rdf:type schema:CreativeWork
254 sg:pub.10.1038/nmeth.1318 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040405189
255 https://doi.org/10.1038/nmeth.1318
256 rdf:type schema:CreativeWork
257 sg:pub.10.1038/nprot.2014.084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010581475
258 https://doi.org/10.1038/nprot.2014.084
259 rdf:type schema:CreativeWork
260 sg:pub.10.1038/nrmicro.2015.24 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005279381
261 https://doi.org/10.1038/nrmicro.2015.24
262 rdf:type schema:CreativeWork
263 https://app.dimensions.ai/details/publication/pub.1077070669 schema:CreativeWork
264 https://app.dimensions.ai/details/publication/pub.1083255199 schema:CreativeWork
265 https://doi.org/10.1002/hlca.192400701122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048519528
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/j.chembiol.2012.07.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025566658
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/j.copbio.2017.02.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084068245
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/j.sbi.2014.08.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050106796
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1021/acssynbio.6b00200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055140892
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1021/sb2000116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011545005
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1039/jr9370001704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024139593
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1073/pnas.120163297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039276805
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1073/pnas.1222159110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000743942
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1073/pnas.91.9.4034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002873189
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1094/mpmi-5-330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060079261
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1094/mpmi.2001.14.8.1006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060080440
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1111/mmi.12566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023205996
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1128/aem.02009-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020443656
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1128/jb.183.1.318-327.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002145874
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1128/jb.183.21.6454-6465.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034214640
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1128/mbio.00932-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049896070
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1146/annurev.phyto.44.013106.145710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013154308
300 rdf:type schema:CreativeWork
301 https://www.grid.ac/institutes/grid.10698.36 schema:alternateName University of North Carolina at Chapel Hill
302 schema:name Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
303 Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
304 Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
305 Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
306 Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
307 rdf:type schema:Organization
308 https://www.grid.ac/institutes/grid.184769.5 schema:alternateName Lawrence Berkeley National Laboratory
309 schema:name Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
310 Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
311 US DOE Joint Genome Institute, Walnut Creek, CA, USA
312 rdf:type schema:Organization
313 https://www.grid.ac/institutes/grid.30064.31 schema:alternateName Washington State University
314 schema:name Department of Plant Pathology, Washington State University, Pullman, WA, USA
315 USDA Agricultural Research Service, Wheat Health, Genetics and Quality, Washington State University, Pullman, WA, USA
316 rdf:type schema:Organization
317 https://www.grid.ac/institutes/grid.451309.a schema:alternateName Joint Genome Institute
318 schema:name US DOE Joint Genome Institute, Walnut Creek, CA, USA
319 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...