Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-06-27

AUTHORS

Shuliang Gao, Yangyang Tong, Zhiqiang Wen, Li Zhu, Mei Ge, Daijie Chen, Yu Jiang, Sheng Yang

ABSTRACT

Yarrowia lipolytica is categorized as a generally recognized as safe (GRAS) organism and is a heavily documented, unconventional yeast that has been widely incorporated into multiple industrial fields to produce valuable biochemicals. This study describes the construction of a CRISPR-Cas9 system for genome editing in Y. lipolytica using a single plasmid (pCAS1yl or pCAS2yl) to transport Cas9 and relevant guide RNA expression cassettes, with or without donor DNA, to target genes. Two Cas9 target genes, TRP1 and PEX10, were repaired by non-homologous end-joining (NHEJ) or homologous recombination, with maximal efficiencies in Y. lipolytica of 85.6 % for the wild-type strain and 94.1 % for the ku70/ku80 double-deficient strain, within 4 days. Simultaneous double and triple multigene editing was achieved with pCAS1yl by NHEJ, with efficiencies of 36.7 or 19.3 %, respectively, and the pCASyl system was successfully expanded to different Y. lipolytica breeding strains. This timesaving method will enable and improve synthetic biology, metabolic engineering and functional genomic studies of Y. lipolytica. More... »

PAGES

1085-1093

References to SciGraph publications

  • 2012-12-09. Efficient homologous recombination with short length flanking fragments in Ku70 deficient Yarrowia lipolytica strains in BIOTECHNOLOGY LETTERS
  • 2012-02-15. RNA-guided genetic silencing systems in bacteria and archaea in NATURE
  • 2013-07-21. Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica in NATURE BIOTECHNOLOGY
  • 2012-05-19. Bioengineering of Oleaginous Yeast Yarrowia lipolytica for Lycopene Production in MICROBIAL CAROTENOIDS FROM FUNGI
  • 2014-09-13. One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica in BIOTECHNOLOGY LETTERS
  • 2011-03-30. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III in NATURE
  • 2014-10-29. Implementation of the CRISPR-Cas9 system in fission yeast in NATURE COMMUNICATIONS
  • 2006-08-23. Mechanism of homologous recombination: mediators and helicases take on regulatory functions in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2014-01-20. Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production in NATURE COMMUNICATIONS
  • 1994-07. Multiple-copy integration in the yeast Yarrowia lipolytica in CURRENT GENETICS
  • 2015-05-12. Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system in CELL DISCOVERY
  • 2013-10-29. The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori in CELL RESEARCH
  • 2013-01-29. RNA-guided editing of bacterial genomes using CRISPR-Cas systems in NATURE BIOTECHNOLOGY
  • 2013-02-20. Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining in CURRENT GENETICS
  • Journal

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10295-016-1789-8

    DOI

    http://dx.doi.org/10.1007/s10295-016-1789-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1008193065

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27349768


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CRISPR-Cas Systems", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA End-Joining Repair", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Editing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Fungal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Fungal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinational DNA Repair", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Yarrowia", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/grid.28056.39", 
              "name": [
                "School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gao", 
            "givenName": "Shuliang", 
            "id": "sg:person.0622366666.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622366666.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Rd, 200234, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/grid.412531.0", 
              "name": [
                "College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Rd, 200234, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tong", 
            "givenName": "Yangyang", 
            "id": "sg:person.014371142351.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014371142351.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/grid.419092.7", 
              "name": [
                "CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wen", 
            "givenName": "Zhiqiang", 
            "id": "sg:person.016532754121.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016532754121.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, 200240, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, 200240, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhu", 
            "givenName": "Li", 
            "id": "sg:person.0741177770.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741177770.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, 200240, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, 200240, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ge", 
            "givenName": "Mei", 
            "id": "sg:person.01012230614.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012230614.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Road, 200040, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/grid.419098.d", 
              "name": [
                "School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China", 
                "Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Road, 200040, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Daijie", 
            "id": "sg:person.0747444707.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747444707.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, 201201, Shanghai, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China", 
                "Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, 201201, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jiang", 
            "givenName": "Yu", 
            "id": "sg:person.01332533554.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332533554.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), 200 North Zhongshan Road, 211816, Nanjing, China", 
              "id": "http://www.grid.ac/institutes/grid.484516.a", 
              "name": [
                "CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China", 
                "Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, 201201, Shanghai, China", 
                "Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), 200 North Zhongshan Road, 211816, Nanjing, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Sheng", 
            "id": "sg:person.011767670320.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011767670320.37"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/978-1-61779-918-1_9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032108959", 
              "https://doi.org/10.1007/978-1-61779-918-1_9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2508", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019888616", 
              "https://doi.org/10.1038/nbt.2508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.146", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007939040", 
              "https://doi.org/10.1038/cr.2013.146"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00326302", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022204649", 
              "https://doi.org/10.1007/bf00326302"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-014-1634-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037821486", 
              "https://doi.org/10.1007/s10529-014-1634-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2622", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008231493", 
              "https://doi.org/10.1038/nbt.2622"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-012-1107-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046289453", 
              "https://doi.org/10.1007/s10529-012-1107-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/celldisc.2015.7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029390352", 
              "https://doi.org/10.1038/celldisc.2015.7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00294-013-0389-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049065515", 
              "https://doi.org/10.1007/s00294-013-0389-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09886", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030591890", 
              "https://doi.org/10.1038/nature09886"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm2008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000968951", 
              "https://doi.org/10.1038/nrm2008"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10886", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053669571", 
              "https://doi.org/10.1038/nature10886"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms4131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040969309", 
              "https://doi.org/10.1038/ncomms4131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms6344", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023944154", 
              "https://doi.org/10.1038/ncomms6344"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-06-27", 
        "datePublishedReg": "2016-06-27", 
        "description": "Yarrowia lipolytica is categorized as a generally recognized as safe (GRAS) organism and is a heavily documented, unconventional yeast that has been widely incorporated into multiple industrial fields to produce valuable biochemicals. This study describes the construction of a CRISPR-Cas9 system for genome editing in Y. lipolytica using a single plasmid (pCAS1yl or pCAS2yl) to transport Cas9 and relevant guide RNA expression cassettes, with or without donor DNA, to target genes. Two Cas9 target genes, TRP1 and PEX10, were repaired by non-homologous end-joining (NHEJ) or homologous recombination, with maximal efficiencies in Y. lipolytica of 85.6\u00a0% for the wild-type strain and 94.1\u00a0% for the ku70/ku80 double-deficient strain, within 4\u00a0days. Simultaneous double and triple multigene editing was achieved with pCAS1yl by NHEJ, with efficiencies of 36.7 or 19.3\u00a0%, respectively, and the pCASyl system was successfully expanded to different Y. lipolytica breeding strains. This timesaving method will enable and improve synthetic biology, metabolic engineering and functional genomic studies of Y. lipolytica.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s10295-016-1789-8", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1412877", 
            "issn": [
              "1367-5435", 
              "1476-5535"
            ], 
            "name": "Journal of Industrial Microbiology & Biotechnology", 
            "publisher": "Oxford University Press (OUP)", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "8", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "43"
          }
        ], 
        "keywords": [
          "Y. lipolytica", 
          "functional genomic studies", 
          "multiplex gene editing", 
          "wild-type strain", 
          "RNA expression cassettes", 
          "genomic studies", 
          "genome editing", 
          "metabolic engineering", 
          "homologous recombination", 
          "target genes", 
          "synthetic biology", 
          "safe organisms", 
          "unconventional yeast", 
          "Yarrowia lipolytica", 
          "gene editing", 
          "expression cassette", 
          "donor DNA", 
          "multigene editing", 
          "valuable biochemicals", 
          "lipolytica", 
          "single plasmid", 
          "CRISPR", 
          "genes", 
          "editing", 
          "multiple industrial fields", 
          "genome", 
          "PEX10", 
          "NHEJ", 
          "Cas9", 
          "breeding", 
          "TRP1", 
          "yeast", 
          "organisms", 
          "biology", 
          "DNA", 
          "strains", 
          "plasmid", 
          "cassette", 
          "maximal efficiency", 
          "recombination", 
          "biochemical", 
          "timesaving method", 
          "industrial fields", 
          "efficiency", 
          "study", 
          "engineering", 
          "system", 
          "days", 
          "method", 
          "field", 
          "construction"
        ], 
        "name": "Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system", 
        "pagination": "1085-1093", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1008193065"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s10295-016-1789-8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "27349768"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s10295-016-1789-8", 
          "https://app.dimensions.ai/details/publication/pub.1008193065"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:35", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_711.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s10295-016-1789-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1789-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1789-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1789-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1789-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    266 TRIPLES      21 PREDICATES      97 URIs      75 LITERALS      14 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s10295-016-1789-8 schema:about N0e31b56515fb4a7d82e309c60bd2bad7
    2 N0e907d0da65e4e669bfb00f0efffdaed
    3 N241877a5a567457f905b1cb048eef20b
    4 N6355845cacbb4cf2b26c6d50ef9b957b
    5 N6e7ec8ddf54e4fe2ab097776bca1024c
    6 Nbc45b26c2a7849a4b55a899731e62b88
    7 Nc02966c4591a4c26ab0f2ef6c5e8364a
    8 anzsrc-for:06
    9 anzsrc-for:0604
    10 schema:author Na90e0d6afae84b53843392fca4c0d65c
    11 schema:citation sg:pub.10.1007/978-1-61779-918-1_9
    12 sg:pub.10.1007/bf00326302
    13 sg:pub.10.1007/s00294-013-0389-7
    14 sg:pub.10.1007/s10529-012-1107-0
    15 sg:pub.10.1007/s10529-014-1634-y
    16 sg:pub.10.1038/celldisc.2015.7
    17 sg:pub.10.1038/cr.2013.146
    18 sg:pub.10.1038/nature09886
    19 sg:pub.10.1038/nature10886
    20 sg:pub.10.1038/nbt.2508
    21 sg:pub.10.1038/nbt.2622
    22 sg:pub.10.1038/ncomms4131
    23 sg:pub.10.1038/ncomms6344
    24 sg:pub.10.1038/nrm2008
    25 schema:datePublished 2016-06-27
    26 schema:datePublishedReg 2016-06-27
    27 schema:description Yarrowia lipolytica is categorized as a generally recognized as safe (GRAS) organism and is a heavily documented, unconventional yeast that has been widely incorporated into multiple industrial fields to produce valuable biochemicals. This study describes the construction of a CRISPR-Cas9 system for genome editing in Y. lipolytica using a single plasmid (pCAS1yl or pCAS2yl) to transport Cas9 and relevant guide RNA expression cassettes, with or without donor DNA, to target genes. Two Cas9 target genes, TRP1 and PEX10, were repaired by non-homologous end-joining (NHEJ) or homologous recombination, with maximal efficiencies in Y. lipolytica of 85.6 % for the wild-type strain and 94.1 % for the ku70/ku80 double-deficient strain, within 4 days. Simultaneous double and triple multigene editing was achieved with pCAS1yl by NHEJ, with efficiencies of 36.7 or 19.3 %, respectively, and the pCASyl system was successfully expanded to different Y. lipolytica breeding strains. This timesaving method will enable and improve synthetic biology, metabolic engineering and functional genomic studies of Y. lipolytica.
    28 schema:genre article
    29 schema:isAccessibleForFree false
    30 schema:isPartOf N0089b1b7e36f4a8e8fd9d6436e4c4893
    31 Nb6812816937d4ac78c14f8e3ec19650c
    32 sg:journal.1412877
    33 schema:keywords CRISPR
    34 Cas9
    35 DNA
    36 NHEJ
    37 PEX10
    38 RNA expression cassettes
    39 TRP1
    40 Y. lipolytica
    41 Yarrowia lipolytica
    42 biochemical
    43 biology
    44 breeding
    45 cassette
    46 construction
    47 days
    48 donor DNA
    49 editing
    50 efficiency
    51 engineering
    52 expression cassette
    53 field
    54 functional genomic studies
    55 gene editing
    56 genes
    57 genome
    58 genome editing
    59 genomic studies
    60 homologous recombination
    61 industrial fields
    62 lipolytica
    63 maximal efficiency
    64 metabolic engineering
    65 method
    66 multigene editing
    67 multiple industrial fields
    68 multiplex gene editing
    69 organisms
    70 plasmid
    71 recombination
    72 safe organisms
    73 single plasmid
    74 strains
    75 study
    76 synthetic biology
    77 system
    78 target genes
    79 timesaving method
    80 unconventional yeast
    81 valuable biochemicals
    82 wild-type strain
    83 yeast
    84 schema:name Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system
    85 schema:pagination 1085-1093
    86 schema:productId N2125ac862e4849568de535e8fe90cbcf
    87 N47f8a06d44a84f66a8012bf96a50c780
    88 N8c149fd8dc0d4113a2551437b8e275f8
    89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008193065
    90 https://doi.org/10.1007/s10295-016-1789-8
    91 schema:sdDatePublished 2022-12-01T06:35
    92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    93 schema:sdPublisher Na26e6dd1036a494dbb3dbadcce46160d
    94 schema:url https://doi.org/10.1007/s10295-016-1789-8
    95 sgo:license sg:explorer/license/
    96 sgo:sdDataset articles
    97 rdf:type schema:ScholarlyArticle
    98 N0089b1b7e36f4a8e8fd9d6436e4c4893 schema:issueNumber 8
    99 rdf:type schema:PublicationIssue
    100 N042f3d385d7c4bd6a6f36ba767ea7bf2 rdf:first sg:person.016532754121.62
    101 rdf:rest Nbad743dc1b48495c9ffdab1e62a72307
    102 N0e31b56515fb4a7d82e309c60bd2bad7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name DNA End-Joining Repair
    104 rdf:type schema:DefinedTerm
    105 N0e907d0da65e4e669bfb00f0efffdaed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name CRISPR-Cas Systems
    107 rdf:type schema:DefinedTerm
    108 N2125ac862e4849568de535e8fe90cbcf schema:name dimensions_id
    109 schema:value pub.1008193065
    110 rdf:type schema:PropertyValue
    111 N241877a5a567457f905b1cb048eef20b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Gene Editing
    113 rdf:type schema:DefinedTerm
    114 N2ed4bed41a934929a3e53b0d2a684ee4 rdf:first sg:person.01012230614.39
    115 rdf:rest Nf44684dec7d7437f847a758f735ee809
    116 N3debd877cc6f4316920a9c575117c88d rdf:first sg:person.014371142351.79
    117 rdf:rest N042f3d385d7c4bd6a6f36ba767ea7bf2
    118 N47f8a06d44a84f66a8012bf96a50c780 schema:name pubmed_id
    119 schema:value 27349768
    120 rdf:type schema:PropertyValue
    121 N6355845cacbb4cf2b26c6d50ef9b957b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Genes, Fungal
    123 rdf:type schema:DefinedTerm
    124 N6dad3d2c939441c49d2e805990659d08 rdf:first sg:person.011767670320.37
    125 rdf:rest rdf:nil
    126 N6e7ec8ddf54e4fe2ab097776bca1024c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Genome, Fungal
    128 rdf:type schema:DefinedTerm
    129 N7c2a1187353f482294f9090321410221 rdf:first sg:person.01332533554.43
    130 rdf:rest N6dad3d2c939441c49d2e805990659d08
    131 N8c149fd8dc0d4113a2551437b8e275f8 schema:name doi
    132 schema:value 10.1007/s10295-016-1789-8
    133 rdf:type schema:PropertyValue
    134 Na26e6dd1036a494dbb3dbadcce46160d schema:name Springer Nature - SN SciGraph project
    135 rdf:type schema:Organization
    136 Na90e0d6afae84b53843392fca4c0d65c rdf:first sg:person.0622366666.54
    137 rdf:rest N3debd877cc6f4316920a9c575117c88d
    138 Nb6812816937d4ac78c14f8e3ec19650c schema:volumeNumber 43
    139 rdf:type schema:PublicationVolume
    140 Nbad743dc1b48495c9ffdab1e62a72307 rdf:first sg:person.0741177770.42
    141 rdf:rest N2ed4bed41a934929a3e53b0d2a684ee4
    142 Nbc45b26c2a7849a4b55a899731e62b88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Recombinational DNA Repair
    144 rdf:type schema:DefinedTerm
    145 Nc02966c4591a4c26ab0f2ef6c5e8364a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Yarrowia
    147 rdf:type schema:DefinedTerm
    148 Nf44684dec7d7437f847a758f735ee809 rdf:first sg:person.0747444707.01
    149 rdf:rest N7c2a1187353f482294f9090321410221
    150 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    151 schema:name Biological Sciences
    152 rdf:type schema:DefinedTerm
    153 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    154 schema:name Genetics
    155 rdf:type schema:DefinedTerm
    156 sg:journal.1412877 schema:issn 1367-5435
    157 1476-5535
    158 schema:name Journal of Industrial Microbiology & Biotechnology
    159 schema:publisher Oxford University Press (OUP)
    160 rdf:type schema:Periodical
    161 sg:person.01012230614.39 schema:affiliation grid-institutes:None
    162 schema:familyName Ge
    163 schema:givenName Mei
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012230614.39
    165 rdf:type schema:Person
    166 sg:person.011767670320.37 schema:affiliation grid-institutes:grid.484516.a
    167 schema:familyName Yang
    168 schema:givenName Sheng
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011767670320.37
    170 rdf:type schema:Person
    171 sg:person.01332533554.43 schema:affiliation grid-institutes:None
    172 schema:familyName Jiang
    173 schema:givenName Yu
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332533554.43
    175 rdf:type schema:Person
    176 sg:person.014371142351.79 schema:affiliation grid-institutes:grid.412531.0
    177 schema:familyName Tong
    178 schema:givenName Yangyang
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014371142351.79
    180 rdf:type schema:Person
    181 sg:person.016532754121.62 schema:affiliation grid-institutes:grid.419092.7
    182 schema:familyName Wen
    183 schema:givenName Zhiqiang
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016532754121.62
    185 rdf:type schema:Person
    186 sg:person.0622366666.54 schema:affiliation grid-institutes:grid.28056.39
    187 schema:familyName Gao
    188 schema:givenName Shuliang
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622366666.54
    190 rdf:type schema:Person
    191 sg:person.0741177770.42 schema:affiliation grid-institutes:None
    192 schema:familyName Zhu
    193 schema:givenName Li
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741177770.42
    195 rdf:type schema:Person
    196 sg:person.0747444707.01 schema:affiliation grid-institutes:grid.419098.d
    197 schema:familyName Chen
    198 schema:givenName Daijie
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747444707.01
    200 rdf:type schema:Person
    201 sg:pub.10.1007/978-1-61779-918-1_9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032108959
    202 https://doi.org/10.1007/978-1-61779-918-1_9
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1007/bf00326302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022204649
    205 https://doi.org/10.1007/bf00326302
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1007/s00294-013-0389-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049065515
    208 https://doi.org/10.1007/s00294-013-0389-7
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1007/s10529-012-1107-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046289453
    211 https://doi.org/10.1007/s10529-012-1107-0
    212 rdf:type schema:CreativeWork
    213 sg:pub.10.1007/s10529-014-1634-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1037821486
    214 https://doi.org/10.1007/s10529-014-1634-y
    215 rdf:type schema:CreativeWork
    216 sg:pub.10.1038/celldisc.2015.7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029390352
    217 https://doi.org/10.1038/celldisc.2015.7
    218 rdf:type schema:CreativeWork
    219 sg:pub.10.1038/cr.2013.146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007939040
    220 https://doi.org/10.1038/cr.2013.146
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1038/nature09886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030591890
    223 https://doi.org/10.1038/nature09886
    224 rdf:type schema:CreativeWork
    225 sg:pub.10.1038/nature10886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053669571
    226 https://doi.org/10.1038/nature10886
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1038/nbt.2508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019888616
    229 https://doi.org/10.1038/nbt.2508
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1038/nbt.2622 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008231493
    232 https://doi.org/10.1038/nbt.2622
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1038/ncomms4131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040969309
    235 https://doi.org/10.1038/ncomms4131
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1038/ncomms6344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023944154
    238 https://doi.org/10.1038/ncomms6344
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1038/nrm2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000968951
    241 https://doi.org/10.1038/nrm2008
    242 rdf:type schema:CreativeWork
    243 grid-institutes:None schema:alternateName Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, 200240, Shanghai, China
    244 Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, 201201, Shanghai, China
    245 schema:name CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China
    246 Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, 200240, Shanghai, China
    247 Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, 201201, Shanghai, China
    248 rdf:type schema:Organization
    249 grid-institutes:grid.28056.39 schema:alternateName School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China
    250 schema:name School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China
    251 rdf:type schema:Organization
    252 grid-institutes:grid.412531.0 schema:alternateName College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Rd, 200234, Shanghai, China
    253 schema:name College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Rd, 200234, Shanghai, China
    254 rdf:type schema:Organization
    255 grid-institutes:grid.419092.7 schema:alternateName CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China
    256 schema:name CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China
    257 rdf:type schema:Organization
    258 grid-institutes:grid.419098.d schema:alternateName Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Road, 200040, Shanghai, China
    259 schema:name School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China
    260 Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Road, 200040, Shanghai, China
    261 rdf:type schema:Organization
    262 grid-institutes:grid.484516.a schema:alternateName Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), 200 North Zhongshan Road, 211816, Nanjing, China
    263 schema:name CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China
    264 Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), 200 North Zhongshan Road, 211816, Nanjing, China
    265 Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, 201201, Shanghai, China
    266 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...