Heterologous expression and characterization of a new heme-catalase in Bacillus subtilis 168 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-06

AUTHORS

Tuyishime Philibert, Zhiming Rao, Taowei Yang, Junping Zhou, Genshu Huang, Komera Irene, Niyomukiza Samuel

ABSTRACT

Reactive oxygen species (ROS) is an inherent consequence to all aerobically living organisms that might lead to the cells being lethal and susceptible to oxidative stress. Bacillus pumilus is characterized by high-resistance oxidative stress that stimulated our interest to investigate the heterologous expression and characterization of heme-catalase as potential biocatalyst. Results indicated that recombinant enzyme significantly exhibited the high catalytic activity of 55,784 U/mg expressed in Bacillus subtilis 168 and 98.097 µmol/min/mg peroxidatic activity, the apparent K m of catalytic activity was 59.6 ± 13 mM with higher turnover rate (K cat = 322.651 × 10(3) s(-1)). The pH dependence of catalatic and peroxidatic activity was pH 7.0 and pH 4.5 respectively with temperature dependence of 40 °C and the recombinant heme-catalase exhibited a strong Fe(2+) preference. It was further revealed that catalase KatX2 improved the resistance oxidative stress of B. subtilis. These findings suggest that this B. pumilus heme-catalase can be considered among the industrially relevant biocatalysts due to its exceptional catalytic rate and high stability and it can be a potential candidate for the improvement of oxidative resistance of industrially produced strains. More... »

PAGES

729-740

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-016-1758-2

DOI

http://dx.doi.org/10.1007/s10295-016-1758-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017163917

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27016935


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Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1758-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1758-2'


 

This table displays all metadata directly associated to this object as RDF triples.

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