Immobilization of nitrilase on bioinspired silica for efficient synthesis of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-05

AUTHORS

Li-Qun Jin, Dong-Jing Guo, Zong-Tong Li, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

In this paper, a simple and effective method using sodium metasilicate as precursor and amine as additive was first reported to immobilize recombinant nitrilase, for efficient production of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile. High immobilization recovery of enzyme activity (above 90 %) was achieved. The immobilized enzyme displayed better thermal stability, pH stability and shelf life compared to free nitrilase. Moreover, it showed excellent reusability and could be recycled up to 16 batches without significant loss in activity. 200 mM 2-hydroxy-4-(methylthio) butanenitrile was completely converted by the immobilized enzyme within 30 min, and the accumulation amount of 2-hydroxy-4-(methylthio) butanoic acid reached 130 mmol/g of immobilized beads after 16 batches. These encouraging results demonstrated the efficiency of the new technology for nitrilase immobilization, which has great potential in preparation of 2-hydroxy-4-(methylthio) butanoic acid. More... »

PAGES

585-593

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-016-1747-5

DOI

http://dx.doi.org/10.1007/s10295-016-1747-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045714915

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26886758


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0912", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Materials Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aminohydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biocatalysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Butyrates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Stability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzymes, Immobilized", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Equipment Reuse", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitriles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Silicon Dioxide", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Zhejiang University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jin", 
        "givenName": "Li-Qun", 
        "id": "sg:person.01105203476.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105203476.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zhejiang University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guo", 
        "givenName": "Dong-Jing", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zhejiang University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Zong-Tong", 
        "id": "sg:person.01023330634.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023330634.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zhejiang University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Zhi-Qiang", 
        "id": "sg:person.01256734541.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zhejiang University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Yu-Guo", 
        "id": "sg:person.01144225405.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1039/c4cc06021k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011794946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000337055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013772071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cctc.201402877", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014863663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibtech.2008.06.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025935196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-11-142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026363248", 
          "https://doi.org/10.1186/1475-2859-11-142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2015.02.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027470155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-004-0189-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035204228", 
          "https://doi.org/10.1007/s10295-004-0189-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-002-1062-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045415936", 
          "https://doi.org/10.1007/s00253-002-1062-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-014-1490-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045942958", 
          "https://doi.org/10.1007/s10295-014-1490-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00449-014-1258-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046086340", 
          "https://doi.org/10.1007/s00449-014-1258-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/adsc.200303016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046127595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c2tb00462c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047124608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2010.03.084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047738915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2005.06.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048141802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcatb.2005.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049004492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00449-014-1137-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049795704", 
          "https://doi.org/10.1007/s00449-014-1137-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/adsc.201500039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050278514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.foodchem.2013.04.081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052719383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jsfa.4434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053728385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cm0102483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055408474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cm0102483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055408474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf048827q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055902899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf048827q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055902899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/op9000065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056289314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/op9000065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056289314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1385272033486486", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069171579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1385272819666150601211349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069173500"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-05", 
    "datePublishedReg": "2016-05-01", 
    "description": "In this paper, a simple and effective method using sodium metasilicate as precursor and amine as additive was first reported to immobilize recombinant nitrilase, for efficient production of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile. High immobilization recovery of enzyme activity (above 90 %) was achieved. The immobilized enzyme displayed better thermal stability, pH stability and shelf life compared to free nitrilase. Moreover, it showed excellent reusability and could be recycled up to 16 batches without significant loss in activity. 200 mM 2-hydroxy-4-(methylthio) butanenitrile was completely converted by the immobilized enzyme within 30 min, and the accumulation amount of 2-hydroxy-4-(methylthio) butanoic acid reached 130 mmol/g of immobilized beads after 16 batches. These encouraging results demonstrated the efficiency of the new technology for nitrilase immobilization, which has great potential in preparation of 2-hydroxy-4-(methylthio) butanoic acid.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10295-016-1747-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096599", 
        "issn": [
          "1367-5435", 
          "1476-5535"
        ], 
        "name": "Journal of Industrial Microbiology & Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "43"
      }
    ], 
    "name": "Immobilization of nitrilase on bioinspired silica for efficient synthesis of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile", 
    "pagination": "585-593", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d9c28da472afc487b4f6b1ba51fa310d5be94340e56107ac9b944d531ad266cd"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26886758"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9705544"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10295-016-1747-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045714915"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10295-016-1747-5", 
      "https://app.dimensions.ai/details/publication/pub.1045714915"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000491.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10295-016-1747-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1747-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1747-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1747-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-016-1747-5'


 

This table displays all metadata directly associated to this object as RDF triples.

219 TRIPLES      21 PREDICATES      64 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10295-016-1747-5 schema:about N00b20893ce9946cfbe858722f0c27ccf
2 N0d26397fd63f490391d5dd26a2815359
3 N317f286888f142589876c4e9248c8cd0
4 N341c150a1a884f25beb0b2d75945df13
5 N5db8443a341f47df827e21b986842165
6 N6114e1118ff44cc488ccd113578707a7
7 N61adf372a49543b5894d60963732cc5a
8 N96bbe4e34eb74c858308659cb2116c11
9 N9fa0f64dc44e4167b6a39c684beff978
10 Nea6669daa35a47aabe186eb41bdf95f5
11 Nf2ce72f154854beea2b6402f1bb57883
12 anzsrc-for:09
13 anzsrc-for:0912
14 schema:author N46cfda2acdbd457ea851656495669d65
15 schema:citation sg:pub.10.1007/s00253-002-1062-0
16 sg:pub.10.1007/s00449-014-1137-1
17 sg:pub.10.1007/s00449-014-1258-6
18 sg:pub.10.1007/s10295-004-0189-7
19 sg:pub.10.1007/s10295-014-1490-8
20 sg:pub.10.1186/1475-2859-11-142
21 https://doi.org/10.1002/adsc.200303016
22 https://doi.org/10.1002/adsc.201500039
23 https://doi.org/10.1002/cctc.201402877
24 https://doi.org/10.1002/jsfa.4434
25 https://doi.org/10.1016/j.biortech.2010.03.084
26 https://doi.org/10.1016/j.biortech.2015.02.038
27 https://doi.org/10.1016/j.foodchem.2013.04.081
28 https://doi.org/10.1016/j.jbiotec.2005.06.018
29 https://doi.org/10.1016/j.molcatb.2005.07.001
30 https://doi.org/10.1016/j.tibtech.2008.06.009
31 https://doi.org/10.1021/cm0102483
32 https://doi.org/10.1021/jf048827q
33 https://doi.org/10.1021/op9000065
34 https://doi.org/10.1039/c2tb00462c
35 https://doi.org/10.1039/c4cc06021k
36 https://doi.org/10.1159/000337055
37 https://doi.org/10.2174/1385272033486486
38 https://doi.org/10.2174/1385272819666150601211349
39 schema:datePublished 2016-05
40 schema:datePublishedReg 2016-05-01
41 schema:description In this paper, a simple and effective method using sodium metasilicate as precursor and amine as additive was first reported to immobilize recombinant nitrilase, for efficient production of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile. High immobilization recovery of enzyme activity (above 90 %) was achieved. The immobilized enzyme displayed better thermal stability, pH stability and shelf life compared to free nitrilase. Moreover, it showed excellent reusability and could be recycled up to 16 batches without significant loss in activity. 200 mM 2-hydroxy-4-(methylthio) butanenitrile was completely converted by the immobilized enzyme within 30 min, and the accumulation amount of 2-hydroxy-4-(methylthio) butanoic acid reached 130 mmol/g of immobilized beads after 16 batches. These encouraging results demonstrated the efficiency of the new technology for nitrilase immobilization, which has great potential in preparation of 2-hydroxy-4-(methylthio) butanoic acid.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree false
45 schema:isPartOf N498b46a507374d94b00a096d167e76d8
46 Ne9a48d9d52954af3aa3fc2b9a6139bed
47 sg:journal.1096599
48 schema:name Immobilization of nitrilase on bioinspired silica for efficient synthesis of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile
49 schema:pagination 585-593
50 schema:productId N0d37882ae7fb4db79c1b4b2c5f4d6586
51 N7d9c6734f36049a6b5a1f1a9fa4f91f2
52 Na670486d55924145850dd99f817e33d0
53 Nbbf558bc9b52405696c2a592f6dcb892
54 Nd41070b17b274e9da99e35669d94f371
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045714915
56 https://doi.org/10.1007/s10295-016-1747-5
57 schema:sdDatePublished 2019-04-10T19:04
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher N0cf574ce0040473895c6622f87a073c3
60 schema:url http://link.springer.com/10.1007/s10295-016-1747-5
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N00b20893ce9946cfbe858722f0c27ccf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Equipment Reuse
66 rdf:type schema:DefinedTerm
67 N0cf574ce0040473895c6622f87a073c3 schema:name Springer Nature - SN SciGraph project
68 rdf:type schema:Organization
69 N0d26397fd63f490391d5dd26a2815359 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Biocatalysis
71 rdf:type schema:DefinedTerm
72 N0d37882ae7fb4db79c1b4b2c5f4d6586 schema:name nlm_unique_id
73 schema:value 9705544
74 rdf:type schema:PropertyValue
75 N1385da2b2d5c42cfb645318398b201ce rdf:first sg:person.01023330634.44
76 rdf:rest N4dd680633a5e457397914e28170700f4
77 N317f286888f142589876c4e9248c8cd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Temperature
79 rdf:type schema:DefinedTerm
80 N341c150a1a884f25beb0b2d75945df13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Silicon Dioxide
82 rdf:type schema:DefinedTerm
83 N46cfda2acdbd457ea851656495669d65 rdf:first sg:person.01105203476.08
84 rdf:rest N7b1904f3a5a74c8f89b9ddec3db6fabb
85 N498b46a507374d94b00a096d167e76d8 schema:volumeNumber 43
86 rdf:type schema:PublicationVolume
87 N4dd680633a5e457397914e28170700f4 rdf:first sg:person.01256734541.85
88 rdf:rest N671e786d4f3b44ceaff7b3a32f3d1db9
89 N5db8443a341f47df827e21b986842165 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Enzymes, Immobilized
91 rdf:type schema:DefinedTerm
92 N6114e1118ff44cc488ccd113578707a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Nitriles
94 rdf:type schema:DefinedTerm
95 N61adf372a49543b5894d60963732cc5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Enzyme Stability
97 rdf:type schema:DefinedTerm
98 N671e786d4f3b44ceaff7b3a32f3d1db9 rdf:first sg:person.01144225405.06
99 rdf:rest rdf:nil
100 N7b1904f3a5a74c8f89b9ddec3db6fabb rdf:first Na8ec1744a817489ebdf9dda7f621b136
101 rdf:rest N1385da2b2d5c42cfb645318398b201ce
102 N7d9c6734f36049a6b5a1f1a9fa4f91f2 schema:name pubmed_id
103 schema:value 26886758
104 rdf:type schema:PropertyValue
105 N96bbe4e34eb74c858308659cb2116c11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Butyrates
107 rdf:type schema:DefinedTerm
108 N9fa0f64dc44e4167b6a39c684beff978 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Hydrogen-Ion Concentration
110 rdf:type schema:DefinedTerm
111 Na670486d55924145850dd99f817e33d0 schema:name dimensions_id
112 schema:value pub.1045714915
113 rdf:type schema:PropertyValue
114 Na8ec1744a817489ebdf9dda7f621b136 schema:affiliation https://www.grid.ac/institutes/grid.469325.f
115 schema:familyName Guo
116 schema:givenName Dong-Jing
117 rdf:type schema:Person
118 Nbbf558bc9b52405696c2a592f6dcb892 schema:name readcube_id
119 schema:value d9c28da472afc487b4f6b1ba51fa310d5be94340e56107ac9b944d531ad266cd
120 rdf:type schema:PropertyValue
121 Nd41070b17b274e9da99e35669d94f371 schema:name doi
122 schema:value 10.1007/s10295-016-1747-5
123 rdf:type schema:PropertyValue
124 Ne9a48d9d52954af3aa3fc2b9a6139bed schema:issueNumber 5
125 rdf:type schema:PublicationIssue
126 Nea6669daa35a47aabe186eb41bdf95f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Recombinant Proteins
128 rdf:type schema:DefinedTerm
129 Nf2ce72f154854beea2b6402f1bb57883 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Aminohydrolases
131 rdf:type schema:DefinedTerm
132 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
133 schema:name Engineering
134 rdf:type schema:DefinedTerm
135 anzsrc-for:0912 schema:inDefinedTermSet anzsrc-for:
136 schema:name Materials Engineering
137 rdf:type schema:DefinedTerm
138 sg:journal.1096599 schema:issn 1367-5435
139 1476-5535
140 schema:name Journal of Industrial Microbiology & Biotechnology
141 rdf:type schema:Periodical
142 sg:person.01023330634.44 schema:affiliation https://www.grid.ac/institutes/grid.469325.f
143 schema:familyName Li
144 schema:givenName Zong-Tong
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023330634.44
146 rdf:type schema:Person
147 sg:person.01105203476.08 schema:affiliation https://www.grid.ac/institutes/grid.469325.f
148 schema:familyName Jin
149 schema:givenName Li-Qun
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105203476.08
151 rdf:type schema:Person
152 sg:person.01144225405.06 schema:affiliation https://www.grid.ac/institutes/grid.469325.f
153 schema:familyName Zheng
154 schema:givenName Yu-Guo
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
156 rdf:type schema:Person
157 sg:person.01256734541.85 schema:affiliation https://www.grid.ac/institutes/grid.469325.f
158 schema:familyName Liu
159 schema:givenName Zhi-Qiang
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85
161 rdf:type schema:Person
162 sg:pub.10.1007/s00253-002-1062-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045415936
163 https://doi.org/10.1007/s00253-002-1062-0
164 rdf:type schema:CreativeWork
165 sg:pub.10.1007/s00449-014-1137-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049795704
166 https://doi.org/10.1007/s00449-014-1137-1
167 rdf:type schema:CreativeWork
168 sg:pub.10.1007/s00449-014-1258-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046086340
169 https://doi.org/10.1007/s00449-014-1258-6
170 rdf:type schema:CreativeWork
171 sg:pub.10.1007/s10295-004-0189-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035204228
172 https://doi.org/10.1007/s10295-004-0189-7
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/s10295-014-1490-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045942958
175 https://doi.org/10.1007/s10295-014-1490-8
176 rdf:type schema:CreativeWork
177 sg:pub.10.1186/1475-2859-11-142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026363248
178 https://doi.org/10.1186/1475-2859-11-142
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1002/adsc.200303016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046127595
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1002/adsc.201500039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050278514
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1002/cctc.201402877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014863663
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1002/jsfa.4434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053728385
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.biortech.2010.03.084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047738915
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.biortech.2015.02.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027470155
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.foodchem.2013.04.081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052719383
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.jbiotec.2005.06.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048141802
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.molcatb.2005.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049004492
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.tibtech.2008.06.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025935196
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1021/cm0102483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055408474
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1021/jf048827q schema:sameAs https://app.dimensions.ai/details/publication/pub.1055902899
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1021/op9000065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056289314
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1039/c2tb00462c schema:sameAs https://app.dimensions.ai/details/publication/pub.1047124608
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1039/c4cc06021k schema:sameAs https://app.dimensions.ai/details/publication/pub.1011794946
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1159/000337055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013772071
211 rdf:type schema:CreativeWork
212 https://doi.org/10.2174/1385272033486486 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069171579
213 rdf:type schema:CreativeWork
214 https://doi.org/10.2174/1385272819666150601211349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069173500
215 rdf:type schema:CreativeWork
216 https://www.grid.ac/institutes/grid.469325.f schema:alternateName Zhejiang University of Technology
217 schema:name Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
218 Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
219 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...