Functional manipulations of the tetramycin positive regulatory gene ttmRIV to enhance the production of tetramycin A and nystatin A1 in ... View Full Text


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Article Info

DATE

2015-09

AUTHORS

Hao Cui, Xianpu Ni, Wei Shao, Jian Su, Jiaqi Su, Jun Ren, Huanzhang Xia

ABSTRACT

A putative regulatory gene ttmRIV located in the tetramycin biosynthetic gene cluster was found in Streptomyces ahygroscopicus. In-frame deletion of ttmRIV led to abolishment of tetramycin and significant enhancement of nystatin A1, whose production reached 2.1-fold of the H42 parental strain. Gene complementation by an integrative plasmid carrying ttmRIV restored tetramycin biosynthesis revealed that ttmRIV was indispensable to tetramycin biosynthesis. Gene expression analysis of the H42 strain and its mutant strain ΔttmRIV via reverse transcriptase-PCR of the tetramycin gene cluster demonstrated that the expression levels of most biosynthetic genes were reduced in ΔttmRIV. Results of electrophoretic mobility shift assays showed that TtmRIV bound the putative promoters of several genes in the tetramycin pathway. Thus, TtmRIV is a pathway-specific positive regulator activating the transcription of the tetramycin gene cluster in S. ahygroscopicus. Providing an additional copy of ttmRIV under the control of the ermEp* promoter in the H42 strain boosted tetramycin A production to 3.3-fold. More... »

PAGES

1273-1282

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-015-1660-3

DOI

http://dx.doi.org/10.1007/s10295-015-1660-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043874019

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26233316


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