An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-11-21

AUTHORS

Nitya Nand Sharma, Monica Sharma, Tek Chand Bhalla

ABSTRACT

Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mgDCW−1, 18.7 U mL−1) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits both substrate and product inhibition effects. In a fed batch reaction at 1 L scale using hyperinduced resting cells corresponding to 10 U mL−1 nitrilase activity (1.5 mgDCW mL−1), a total of 123.11 g nicotinic acid was produced at a rate of 24 g h−1 gDCW−1. More... »

PAGES

1235-1243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-010-0902-7

DOI

http://dx.doi.org/10.1007/s10295-010-0902-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002718146

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21104103


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