Ontology type: schema:ScholarlyArticle
2010-07-18
AUTHORSYa-Ping Xue, Sai-Zhen Xu, Zhi-Qiang Liu, Yu-Guo Zheng, Yin-Chu Shen
ABSTRACT(R)-(−)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl–ultraviolet irradiation and low energy N+ ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8 U g−1, in comparison with wild-type strain WT10 of 53.09 U g−1. The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7–8.5. The optimal temperature was 35°C. The highest production rate reached 9.3 mmol h−1 g−1. The results showed that mutant strain A. faecalis ZJUTB10 was a new candidate for efficient R-MA production from (R,S)-mandelonitrile and could potentially be used in industrial production. More... »
PAGES337-345
http://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6
DOIhttp://dx.doi.org/10.1007/s10295-010-0778-6
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1046930955
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/20640871
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Engineering",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0914",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Resources Engineering and Extractive Metallurgy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Acetonitriles",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Alcaligenes faecalis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Aminohydrolases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biocatalysis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biotechnology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hydrogen-Ion Concentration",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hydrolysis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mandelic Acids",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Phylogeny",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Ribosomal, 16S",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Soil Microbiology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Temperature",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Xue",
"givenName": "Ya-Ping",
"id": "sg:person.0744724672.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Xu",
"givenName": "Sai-Zhen",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Liu",
"givenName": "Zhi-Qiang",
"id": "sg:person.01256734541.85",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Zheng",
"givenName": "Yu-Guo",
"id": "sg:person.01144225405.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China",
"id": "http://www.grid.ac/institutes/grid.469325.f",
"name": [
"Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
],
"type": "Organization"
},
"familyName": "Shen",
"givenName": "Yin-Chu",
"id": "sg:person.01072704134.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072704134.44"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s11244-005-3817-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012803549",
"https://doi.org/10.1007/s11244-005-3817-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/10_2008_25",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031952913",
"https://doi.org/10.1007/10_2008_25"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-005-0255-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008519496",
"https://doi.org/10.1007/s00253-005-0255-8"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf01021248",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000352851",
"https://doi.org/10.1007/bf01021248"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00449-005-0013-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043476050",
"https://doi.org/10.1007/s00449-005-0013-4"
],
"type": "CreativeWork"
}
],
"datePublished": "2010-07-18",
"datePublishedReg": "2010-07-18",
"description": "Abstract(R)-(\u2212)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl\u2013ultraviolet irradiation and low energy N+ ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8\u00a0U\u00a0g\u22121, in comparison with wild-type strain WT10 of 53.09\u00a0U\u00a0g\u22121. The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7\u20138.5. The optimal temperature was 35\u00b0C. The highest production rate reached 9.3\u00a0mmol\u00a0h\u22121\u00a0g\u22121. The results showed that mutant strain A. faecalis ZJUTB10 was a new candidate for efficient R-MA production from (R,S)-mandelonitrile and could potentially be used in industrial production.",
"genre": "article",
"id": "sg:pub.10.1007/s10295-010-0778-6",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1412877",
"issn": [
"0169-4146",
"1367-5435"
],
"name": "Journal of Industrial Microbiology & Biotechnology",
"publisher": "Oxford University Press (OUP)",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "38"
}
],
"keywords": [
"ion beam implantation technique",
"enantioselective biocatalytic hydrolysis",
"high production rate",
"industrial applications",
"production rate",
"industrial production",
"implantation technique",
"high productivity",
"reaction conditions",
"temperature",
"biocatalytic hydrolysis",
"low energy",
"broad uses",
"R-MA",
"energy",
"optimal temperature",
"strains",
"applications",
"production",
"more attention",
"irradiation",
"technique",
"conditions",
"work",
"order",
"results",
"comparison",
"hydrolysis",
"optimum",
"new candidates",
"candidates",
"rate",
"important intermediate",
"productivity",
"attention",
"acid",
"Alcaligenes faecalis",
"mandelic acid",
"uses",
"mandelonitrile",
"activity",
"excellent enantioselectivities",
"intermediates",
"nitrilase activity",
"WT10",
"enantioselectivity",
"specific activity",
"faecalis",
"mutant strain",
"mutagenesis"
],
"name": "Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(\u2212)-mandelic acid by a newly isolated mutant strain",
"pagination": "337-345",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1046930955"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s10295-010-0778-6"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"20640871"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s10295-010-0778-6",
"https://app.dimensions.ai/details/publication/pub.1046930955"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:58",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_517.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s10295-010-0778-6"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'
This table displays all metadata directly associated to this object as RDF triples.
214 TRIPLES
21 PREDICATES
94 URIs
81 LITERALS
21 BLANK NODES