Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(−)-mandelic acid by a newly isolated mutant strain View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-07-18

AUTHORS

Ya-Ping Xue, Sai-Zhen Xu, Zhi-Qiang Liu, Yu-Guo Zheng, Yin-Chu Shen

ABSTRACT

(R)-(−)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl–ultraviolet irradiation and low energy N+ ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8 U g−1, in comparison with wild-type strain WT10 of 53.09 U g−1. The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7–8.5. The optimal temperature was 35°C. The highest production rate reached 9.3 mmol h−1 g−1. The results showed that mutant strain A. faecalis ZJUTB10 was a new candidate for efficient R-MA production from (R,S)-mandelonitrile and could potentially be used in industrial production. More... »

PAGES

337-345

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6

DOI

http://dx.doi.org/10.1007/s10295-010-0778-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046930955

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20640871


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