Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(−)-mandelic acid by a newly isolated mutant strain View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-07-18

AUTHORS

Ya-Ping Xue, Sai-Zhen Xu, Zhi-Qiang Liu, Yu-Guo Zheng, Yin-Chu Shen

ABSTRACT

(R)-(−)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl–ultraviolet irradiation and low energy N+ ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8 U g−1, in comparison with wild-type strain WT10 of 53.09 U g−1. The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7–8.5. The optimal temperature was 35°C. The highest production rate reached 9.3 mmol h−1 g−1. The results showed that mutant strain A. faecalis ZJUTB10 was a new candidate for efficient R-MA production from (R,S)-mandelonitrile and could potentially be used in industrial production. More... »

PAGES

337-345

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6

DOI

http://dx.doi.org/10.1007/s10295-010-0778-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046930955

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20640871


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0914", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Resources Engineering and Extractive Metallurgy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetonitriles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alcaligenes faecalis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aminohydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biocatalysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mandelic Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phylogeny", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Ribosomal, 16S", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Soil Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xue", 
        "givenName": "Ya-Ping", 
        "id": "sg:person.0744724672.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Sai-Zhen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Zhi-Qiang", 
        "id": "sg:person.01256734541.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Yu-Guo", 
        "id": "sg:person.01144225405.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shen", 
        "givenName": "Yin-Chu", 
        "id": "sg:person.01072704134.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072704134.44"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s11244-005-3817-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012803549", 
          "https://doi.org/10.1007/s11244-005-3817-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/10_2008_25", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031952913", 
          "https://doi.org/10.1007/10_2008_25"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0255-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008519496", 
          "https://doi.org/10.1007/s00253-005-0255-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01021248", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000352851", 
          "https://doi.org/10.1007/bf01021248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00449-005-0013-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043476050", 
          "https://doi.org/10.1007/s00449-005-0013-4"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-07-18", 
    "datePublishedReg": "2010-07-18", 
    "description": "Abstract(R)-(\u2212)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl\u2013ultraviolet irradiation and low energy N+ ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8\u00a0U\u00a0g\u22121, in comparison with wild-type strain WT10 of 53.09\u00a0U\u00a0g\u22121. The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7\u20138.5. The optimal temperature was 35\u00b0C. The highest production rate reached 9.3\u00a0mmol\u00a0h\u22121\u00a0g\u22121. The results showed that mutant strain A. faecalis ZJUTB10 was a new candidate for efficient R-MA production from (R,S)-mandelonitrile and could potentially be used in industrial production.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s10295-010-0778-6", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1412877", 
        "issn": [
          "0169-4146", 
          "1367-5435"
        ], 
        "name": "Journal of Industrial Microbiology & Biotechnology", 
        "publisher": "Oxford University Press (OUP)", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "38"
      }
    ], 
    "keywords": [
      "ion beam implantation technique", 
      "enantioselective biocatalytic hydrolysis", 
      "high production rate", 
      "industrial applications", 
      "production rate", 
      "industrial production", 
      "implantation technique", 
      "high productivity", 
      "reaction conditions", 
      "temperature", 
      "biocatalytic hydrolysis", 
      "low energy", 
      "broad uses", 
      "R-MA", 
      "energy", 
      "optimal temperature", 
      "strains", 
      "applications", 
      "production", 
      "more attention", 
      "irradiation", 
      "technique", 
      "conditions", 
      "work", 
      "order", 
      "results", 
      "comparison", 
      "hydrolysis", 
      "optimum", 
      "new candidates", 
      "candidates", 
      "rate", 
      "important intermediate", 
      "productivity", 
      "attention", 
      "acid", 
      "Alcaligenes faecalis", 
      "mandelic acid", 
      "uses", 
      "mandelonitrile", 
      "activity", 
      "excellent enantioselectivities", 
      "intermediates", 
      "nitrilase activity", 
      "WT10", 
      "enantioselectivity", 
      "specific activity", 
      "faecalis", 
      "mutant strain", 
      "mutagenesis"
    ], 
    "name": "Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(\u2212)-mandelic acid by a newly isolated mutant strain", 
    "pagination": "337-345", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046930955"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10295-010-0778-6"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20640871"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10295-010-0778-6", 
      "https://app.dimensions.ai/details/publication/pub.1046930955"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_517.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s10295-010-0778-6"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-010-0778-6'


 

This table displays all metadata directly associated to this object as RDF triples.

214 TRIPLES      21 PREDICATES      94 URIs      81 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10295-010-0778-6 schema:about N12ffaaf9318f4e8d9f607a88d9cb2287
2 N1b0f70473b74489398afc619b3e9ff12
3 N307cc63111c84bf98e634ca8e26172c1
4 N4319008dfa02485f977a349b8ec6616e
5 N6280e01e067b4138965562b4d62e9644
6 N6bab3a81271a4506a9ae7cb5db3c8bc6
7 Nb06ff7e9c14d41af82ff0b49f1cf79e6
8 Nbbd91397e1c24473a140b5e98aaa14d0
9 Nc073da743cd14b82b1134ca8093a18c4
10 Nd7bdce3e645a4db3aed3a38d1ba02eee
11 Ne39907e71abc4c2983e96181cc6850ac
12 Ne626bf094127458299c9dd5fd471bc58
13 Ne651469558e34e28ade596e874a84ce9
14 Nf5f5b5107592421497ca21d0b259ab76
15 anzsrc-for:09
16 anzsrc-for:0914
17 schema:author N717443d8f2ad4ec99be117c80dac1889
18 schema:citation sg:pub.10.1007/10_2008_25
19 sg:pub.10.1007/bf01021248
20 sg:pub.10.1007/s00253-005-0255-8
21 sg:pub.10.1007/s00449-005-0013-4
22 sg:pub.10.1007/s11244-005-3817-1
23 schema:datePublished 2010-07-18
24 schema:datePublishedReg 2010-07-18
25 schema:description Abstract(R)-(−)-Mandelic acid (R-MA) is an important intermediate with broad uses. Recently, R-MA production using nitrilase has been gaining more and more attention due to its higher productivity and enantioselectivity. In this work, a new bacterium WT10, which exhibited favorable nitrilase activity and excellent enantioselectivity for production of R-MA by enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile, was isolated and identified as a strain of Alcaligenes faecalis. In order to improve its nitrilase activity for industrial application, the wild-type strain WT10 was further subjected to mutagenesis using a combined LiCl–ultraviolet irradiation and low energy N+ ion beams implantation technique. A valuable mutant strain A. faecalis ZJUTB10 was obtained. The nitrilase specific activity of the mutant strain was greatly improved up to 350.8 U g−1, in comparison with wild-type strain WT10 of 53.09 U g−1. The reaction conditions for R-MA production by mutant strain A. faecalis ZJUTB10 were also optimized. Nitrilase activity in mutant strain showed a broad pH optimum at pH 7.7–8.5. The optimal temperature was 35°C. The highest production rate reached 9.3 mmol h−1 g−1. The results showed that mutant strain A. faecalis ZJUTB10 was a new candidate for efficient R-MA production from (R,S)-mandelonitrile and could potentially be used in industrial production.
26 schema:genre article
27 schema:isAccessibleForFree false
28 schema:isPartOf N5ee61ec276f54ad6abf51f1d9e668b94
29 N944b967424a04aed9c13cf1431b27a3a
30 sg:journal.1412877
31 schema:keywords Alcaligenes faecalis
32 R-MA
33 WT10
34 acid
35 activity
36 applications
37 attention
38 biocatalytic hydrolysis
39 broad uses
40 candidates
41 comparison
42 conditions
43 enantioselective biocatalytic hydrolysis
44 enantioselectivity
45 energy
46 excellent enantioselectivities
47 faecalis
48 high production rate
49 high productivity
50 hydrolysis
51 implantation technique
52 important intermediate
53 industrial applications
54 industrial production
55 intermediates
56 ion beam implantation technique
57 irradiation
58 low energy
59 mandelic acid
60 mandelonitrile
61 more attention
62 mutagenesis
63 mutant strain
64 new candidates
65 nitrilase activity
66 optimal temperature
67 optimum
68 order
69 production
70 production rate
71 productivity
72 rate
73 reaction conditions
74 results
75 specific activity
76 strains
77 technique
78 temperature
79 uses
80 work
81 schema:name Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(−)-mandelic acid by a newly isolated mutant strain
82 schema:pagination 337-345
83 schema:productId N1f30b8f4b1574d19b3f7978462c54d86
84 N897e00880c554f08b06d48f2057cf580
85 Nb47f64a827e24129a9baeb861faa0fcb
86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046930955
87 https://doi.org/10.1007/s10295-010-0778-6
88 schema:sdDatePublished 2022-08-04T16:58
89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
90 schema:sdPublisher Nc788412accee4879a00bb0b988a3759d
91 schema:url https://doi.org/10.1007/s10295-010-0778-6
92 sgo:license sg:explorer/license/
93 sgo:sdDataset articles
94 rdf:type schema:ScholarlyArticle
95 N12ffaaf9318f4e8d9f607a88d9cb2287 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Temperature
97 rdf:type schema:DefinedTerm
98 N1b0f70473b74489398afc619b3e9ff12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Hydrogen-Ion Concentration
100 rdf:type schema:DefinedTerm
101 N1f30b8f4b1574d19b3f7978462c54d86 schema:name doi
102 schema:value 10.1007/s10295-010-0778-6
103 rdf:type schema:PropertyValue
104 N307cc63111c84bf98e634ca8e26172c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Acetonitriles
106 rdf:type schema:DefinedTerm
107 N4319008dfa02485f977a349b8ec6616e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Molecular Sequence Data
109 rdf:type schema:DefinedTerm
110 N5ee61ec276f54ad6abf51f1d9e668b94 schema:volumeNumber 38
111 rdf:type schema:PublicationVolume
112 N6280e01e067b4138965562b4d62e9644 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Aminohydrolases
114 rdf:type schema:DefinedTerm
115 N6bab3a81271a4506a9ae7cb5db3c8bc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Soil Microbiology
117 rdf:type schema:DefinedTerm
118 N717443d8f2ad4ec99be117c80dac1889 rdf:first sg:person.0744724672.04
119 rdf:rest Nfc392827a1a74e73a5e9de243ab35f45
120 N7bb92664cc83424d9d48af0987a74912 rdf:first sg:person.01256734541.85
121 rdf:rest N89e10c1b9c6742099d0cc7c8255d1fe7
122 N897e00880c554f08b06d48f2057cf580 schema:name pubmed_id
123 schema:value 20640871
124 rdf:type schema:PropertyValue
125 N89e10c1b9c6742099d0cc7c8255d1fe7 rdf:first sg:person.01144225405.06
126 rdf:rest N9e68f2d0539942999abaeb0ce48bedf9
127 N944b967424a04aed9c13cf1431b27a3a schema:issueNumber 2
128 rdf:type schema:PublicationIssue
129 N979e8c28e0bc44018b7477957084d38f schema:affiliation grid-institutes:grid.469325.f
130 schema:familyName Xu
131 schema:givenName Sai-Zhen
132 rdf:type schema:Person
133 N9e68f2d0539942999abaeb0ce48bedf9 rdf:first sg:person.01072704134.44
134 rdf:rest rdf:nil
135 Nb06ff7e9c14d41af82ff0b49f1cf79e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Phylogeny
137 rdf:type schema:DefinedTerm
138 Nb47f64a827e24129a9baeb861faa0fcb schema:name dimensions_id
139 schema:value pub.1046930955
140 rdf:type schema:PropertyValue
141 Nbbd91397e1c24473a140b5e98aaa14d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name RNA, Ribosomal, 16S
143 rdf:type schema:DefinedTerm
144 Nc073da743cd14b82b1134ca8093a18c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Hydrolysis
146 rdf:type schema:DefinedTerm
147 Nc788412accee4879a00bb0b988a3759d schema:name Springer Nature - SN SciGraph project
148 rdf:type schema:Organization
149 Nd7bdce3e645a4db3aed3a38d1ba02eee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Mandelic Acids
151 rdf:type schema:DefinedTerm
152 Ne39907e71abc4c2983e96181cc6850ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Biotechnology
154 rdf:type schema:DefinedTerm
155 Ne626bf094127458299c9dd5fd471bc58 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Mutation
157 rdf:type schema:DefinedTerm
158 Ne651469558e34e28ade596e874a84ce9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Biocatalysis
160 rdf:type schema:DefinedTerm
161 Nf5f5b5107592421497ca21d0b259ab76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Alcaligenes faecalis
163 rdf:type schema:DefinedTerm
164 Nfc392827a1a74e73a5e9de243ab35f45 rdf:first N979e8c28e0bc44018b7477957084d38f
165 rdf:rest N7bb92664cc83424d9d48af0987a74912
166 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
167 schema:name Engineering
168 rdf:type schema:DefinedTerm
169 anzsrc-for:0914 schema:inDefinedTermSet anzsrc-for:
170 schema:name Resources Engineering and Extractive Metallurgy
171 rdf:type schema:DefinedTerm
172 sg:journal.1412877 schema:issn 0169-4146
173 1367-5435
174 schema:name Journal of Industrial Microbiology & Biotechnology
175 schema:publisher Oxford University Press (OUP)
176 rdf:type schema:Periodical
177 sg:person.01072704134.44 schema:affiliation grid-institutes:grid.469325.f
178 schema:familyName Shen
179 schema:givenName Yin-Chu
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072704134.44
181 rdf:type schema:Person
182 sg:person.01144225405.06 schema:affiliation grid-institutes:grid.469325.f
183 schema:familyName Zheng
184 schema:givenName Yu-Guo
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
186 rdf:type schema:Person
187 sg:person.01256734541.85 schema:affiliation grid-institutes:grid.469325.f
188 schema:familyName Liu
189 schema:givenName Zhi-Qiang
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85
191 rdf:type schema:Person
192 sg:person.0744724672.04 schema:affiliation grid-institutes:grid.469325.f
193 schema:familyName Xue
194 schema:givenName Ya-Ping
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04
196 rdf:type schema:Person
197 sg:pub.10.1007/10_2008_25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031952913
198 https://doi.org/10.1007/10_2008_25
199 rdf:type schema:CreativeWork
200 sg:pub.10.1007/bf01021248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000352851
201 https://doi.org/10.1007/bf01021248
202 rdf:type schema:CreativeWork
203 sg:pub.10.1007/s00253-005-0255-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008519496
204 https://doi.org/10.1007/s00253-005-0255-8
205 rdf:type schema:CreativeWork
206 sg:pub.10.1007/s00449-005-0013-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043476050
207 https://doi.org/10.1007/s00449-005-0013-4
208 rdf:type schema:CreativeWork
209 sg:pub.10.1007/s11244-005-3817-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012803549
210 https://doi.org/10.1007/s11244-005-3817-1
211 rdf:type schema:CreativeWork
212 grid-institutes:grid.469325.f schema:alternateName Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People’s Republic of China
213 schema:name Institute of Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People’s Republic of China
214 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...