Purification and characterization of chitinases from Paecilomycesvariotii DG-3 parasitizing on Meloidogyne incognita eggs View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-02

AUTHORS

Van-Nam Nguyen, In-Jae Oh, Young-Ju Kim, Kil-Yong Kim, Young-Cheol Kim, Ro-Dong Park

ABSTRACT

Two extracellular chitinases were purified from Paecilomyces variotii DG-3, a chitinase producer and a nematode egg-parasitic fungus, to homogeneity by DEAE Sephadex A-50 and Sephadex G-100 chromatography. The purified enzymes were a monomer with an apparent molecular mass of 32 kDa (Chi32) and 46 kDa (Chi46), respectively, and showed chitinase activity bands with 0.01% glycol chitin as a substrate after SDS-PAGE. The first 20 and 15 N-terminal amino acid sequences of Chi32 and Chi46 were determined to be Asp-Pro-Typ-Gln-Thr-Asn-Val-Val-Tyr-Thr-Gly-Gln-Asp-Phe-Val-Ser-Pro-Asp-Leu-Phe and Asp-Ala-X-X-Tyr-Arg-Ser-Val-Ala-Tyr-Phe-Val-Asn-Trp-Ala, respectively. Optimal temperature and pH of the Chi32 and Chi46 were found to be both 60 degrees C, and 2.5 and 3.0, respectively. Chi32 was almost inhibited by metal ions Ag(+) and Hg(2+) while Chi46 by Hg(2+) and Pb(2+) at a 10 mM concentration but both enzymes were enhanced by 1 mM concentration of Co(2+). On analyzing the hydrolyzates of chitin oligomers [(GlcNAc)( n ), n = 2-6)], it was considered that Chi32 degraded chitin oligomers as an exo-type chitinase while Chi46 as an endo-type chitinase. More... »

PAGES

195

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10295-008-0485-8

DOI

http://dx.doi.org/10.1007/s10295-008-0485-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048615046

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18936994


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0302", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Inorganic Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chitinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Host-Parasite Interactions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hypocreales", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tylenchoidea", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nguyen", 
        "givenName": "Van-Nam", 
        "id": "sg:person.01244206253.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244206253.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oh", 
        "givenName": "In-Jae", 
        "id": "sg:person.01334100633.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334100633.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Young-Ju", 
        "id": "sg:person.01061644453.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061644453.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Kil-Yong", 
        "id": "sg:person.01144400066.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144400066.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Division of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University, 500-757, Gwangju, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Young-Cheol", 
        "id": "sg:person.01163670477.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163670477.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, South Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Park", 
        "givenName": "Ro-Dong", 
        "id": "sg:person.01026766755.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026766755.10"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1271/bbb1961.51.1189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000960547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2800309", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002668698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2800309", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002668698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0183-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002750710", 
          "https://doi.org/10.1007/s00253-005-0183-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0183-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002750710", 
          "https://doi.org/10.1007/s00253-005-0183-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-372180-8.50042-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004812051"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jipa.1999.4843", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005240131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1993.1010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006799025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.resmic.2004.07.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007736406"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2007.05955.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011744769"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0031182000043158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013975928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0031182000043158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013975928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-003-1490-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016763631", 
          "https://doi.org/10.1007/s00253-003-1490-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbapap.2005.01.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017784534"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb1961.35.1154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020765446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00792-003-0338-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021178020", 
          "https://doi.org/10.1007/s00792-003-0338-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00284-005-0412-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025843459", 
          "https://doi.org/10.1007/s00284-005-0412-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00284-005-0412-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025843459", 
          "https://doi.org/10.1007/s00284-005-0412-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biocontrol.2004.07.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026985755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/w05-020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028135799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11274-004-4797-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028581289", 
          "https://doi.org/10.1007/s11274-004-4797-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11274-004-4797-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028581289", 
          "https://doi.org/10.1007/s11274-004-4797-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(89)90653-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028872847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2005.04994.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031413968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2005.04994.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031413968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1008964324425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032143255", 
          "https://doi.org/10.1023/a:1008964324425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/m69-122", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032840353"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(00)00134-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034421903"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1163/1568541041218004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034748522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1163/1568541041218004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034748522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/09583150701668658", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034980465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-004-1877-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035526387", 
          "https://doi.org/10.1007/s00253-004-1877-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-004-1877-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035526387", 
          "https://doi.org/10.1007/s00253-004-1877-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/fgbi.2001.1312", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039849553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(00)00362-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040704567"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00284-006-0078-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041235368", 
          "https://doi.org/10.1007/s00284-006-0078-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00284-006-0078-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041235368", 
          "https://doi.org/10.1007/s00284-006-0078-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pep.2003.07.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041415575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pep.2003.07.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041415575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.1993.tb00004.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043775195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.1993.tb00004.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043775195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.9.3.544", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045440855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.9.3.544", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045440855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(90)82009-q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045878957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00284-007-9033-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046017344", 
          "https://doi.org/10.1007/s00284-007-9033-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/fgbi.1998.1111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049807053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2225/vol4-issue3-fulltext-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069345679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077202108", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1201/9781420040821", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1095907050"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-02", 
    "datePublishedReg": "2009-02-01", 
    "description": "Two extracellular chitinases were purified from Paecilomyces variotii DG-3, a chitinase producer and a nematode egg-parasitic fungus, to homogeneity by DEAE Sephadex A-50 and Sephadex G-100 chromatography. The purified enzymes were a monomer with an apparent molecular mass of 32 kDa (Chi32) and 46 kDa (Chi46), respectively, and showed chitinase activity bands with 0.01% glycol chitin as a substrate after SDS-PAGE. The first 20 and 15 N-terminal amino acid sequences of Chi32 and Chi46 were determined to be Asp-Pro-Typ-Gln-Thr-Asn-Val-Val-Tyr-Thr-Gly-Gln-Asp-Phe-Val-Ser-Pro-Asp-Leu-Phe and Asp-Ala-X-X-Tyr-Arg-Ser-Val-Ala-Tyr-Phe-Val-Asn-Trp-Ala, respectively. Optimal temperature and pH of the Chi32 and Chi46 were found to be both 60 degrees C, and 2.5 and 3.0, respectively. Chi32 was almost inhibited by metal ions Ag(+) and Hg(2+) while Chi46 by Hg(2+) and Pb(2+) at a 10 mM concentration but both enzymes were enhanced by 1 mM concentration of Co(2+). On analyzing the hydrolyzates of chitin oligomers [(GlcNAc)( n ), n = 2-6)], it was considered that Chi32 degraded chitin oligomers as an exo-type chitinase while Chi46 as an endo-type chitinase.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10295-008-0485-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096599", 
        "issn": [
          "1367-5435", 
          "1476-5535"
        ], 
        "name": "Journal of Industrial Microbiology & Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "36"
      }
    ], 
    "name": "Purification and characterization of chitinases from Paecilomycesvariotii DG-3 parasitizing on Meloidogyne incognita eggs", 
    "pagination": "195", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e797d2853dfa7bddc0d2f827bd9594ad595c80af6c955f78f268ba8bc4de92f8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18936994"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9705544"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10295-008-0485-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1048615046"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10295-008-0485-8", 
      "https://app.dimensions.ai/details/publication/pub.1048615046"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13078_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10295-008-0485-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10295-008-0485-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10295-008-0485-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10295-008-0485-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10295-008-0485-8'


 

This table displays all metadata directly associated to this object as RDF triples.

267 TRIPLES      21 PREDICATES      77 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10295-008-0485-8 schema:about N60f7b13c59744ee391a2f6419b64b0e6
2 N8969120284144f7bb4086653a453206d
3 N91cf34ee2acd40b2a3d4c1e96ea403de
4 N948523dee22a496c8d1ed17162eb4cd7
5 Na55e9b6627bb4446865ef313de964949
6 Nc170067a2fac48fd93f53b6e246e51b4
7 Nc66cfe524c52455c97c91c7a27ece576
8 Ncb429c13ee9b4b44b478f3c7ccf74b93
9 Nd299489c69b2480586fd08391f885821
10 anzsrc-for:03
11 anzsrc-for:0302
12 schema:author N49285d3010dc4125b47801b3cdd51872
13 schema:citation sg:pub.10.1007/s00253-003-1490-5
14 sg:pub.10.1007/s00253-004-1877-y
15 sg:pub.10.1007/s00253-005-0183-7
16 sg:pub.10.1007/s00284-005-0412-4
17 sg:pub.10.1007/s00284-006-0078-6
18 sg:pub.10.1007/s00284-007-9033-4
19 sg:pub.10.1007/s00792-003-0338-3
20 sg:pub.10.1007/s11274-004-4797-1
21 sg:pub.10.1023/a:1008964324425
22 sg:pub.10.1038/227680a0
23 https://app.dimensions.ai/details/publication/pub.1077202108
24 https://doi.org/10.1006/abio.1993.1010
25 https://doi.org/10.1006/fgbi.1998.1111
26 https://doi.org/10.1006/fgbi.2001.1312
27 https://doi.org/10.1006/jipa.1999.4843
28 https://doi.org/10.1016/0003-2697(76)90527-3
29 https://doi.org/10.1016/0003-2697(89)90653-2
30 https://doi.org/10.1016/0076-6879(90)82009-q
31 https://doi.org/10.1016/b978-0-12-372180-8.50042-1
32 https://doi.org/10.1016/j.bbapap.2005.01.002
33 https://doi.org/10.1016/j.biocontrol.2004.07.011
34 https://doi.org/10.1016/j.pep.2003.07.007
35 https://doi.org/10.1016/j.resmic.2004.07.003
36 https://doi.org/10.1016/s0141-0229(00)00134-4
37 https://doi.org/10.1016/s0141-0229(00)00362-8
38 https://doi.org/10.1017/s0031182000043158
39 https://doi.org/10.1042/bj2800309
40 https://doi.org/10.1080/09583150701668658
41 https://doi.org/10.1110/ps.9.3.544
42 https://doi.org/10.1111/j.1574-6976.1993.tb00004.x
43 https://doi.org/10.1111/j.1742-4658.2005.04994.x
44 https://doi.org/10.1111/j.1742-4658.2007.05955.x
45 https://doi.org/10.1139/m69-122
46 https://doi.org/10.1139/w05-020
47 https://doi.org/10.1163/1568541041218004
48 https://doi.org/10.1201/9781420040821
49 https://doi.org/10.1271/bbb1961.35.1154
50 https://doi.org/10.1271/bbb1961.51.1189
51 https://doi.org/10.2225/vol4-issue3-fulltext-7
52 schema:datePublished 2009-02
53 schema:datePublishedReg 2009-02-01
54 schema:description Two extracellular chitinases were purified from Paecilomyces variotii DG-3, a chitinase producer and a nematode egg-parasitic fungus, to homogeneity by DEAE Sephadex A-50 and Sephadex G-100 chromatography. The purified enzymes were a monomer with an apparent molecular mass of 32 kDa (Chi32) and 46 kDa (Chi46), respectively, and showed chitinase activity bands with 0.01% glycol chitin as a substrate after SDS-PAGE. The first 20 and 15 N-terminal amino acid sequences of Chi32 and Chi46 were determined to be Asp-Pro-Typ-Gln-Thr-Asn-Val-Val-Tyr-Thr-Gly-Gln-Asp-Phe-Val-Ser-Pro-Asp-Leu-Phe and Asp-Ala-X-X-Tyr-Arg-Ser-Val-Ala-Tyr-Phe-Val-Asn-Trp-Ala, respectively. Optimal temperature and pH of the Chi32 and Chi46 were found to be both 60 degrees C, and 2.5 and 3.0, respectively. Chi32 was almost inhibited by metal ions Ag(+) and Hg(2+) while Chi46 by Hg(2+) and Pb(2+) at a 10 mM concentration but both enzymes were enhanced by 1 mM concentration of Co(2+). On analyzing the hydrolyzates of chitin oligomers [(GlcNAc)( n ), n = 2-6)], it was considered that Chi32 degraded chitin oligomers as an exo-type chitinase while Chi46 as an endo-type chitinase.
55 schema:genre research_article
56 schema:inLanguage en
57 schema:isAccessibleForFree false
58 schema:isPartOf N6b14505491c44078a41c77c133e23454
59 Ncb264b0866754b629e163291cbffb85e
60 sg:journal.1096599
61 schema:name Purification and characterization of chitinases from Paecilomycesvariotii DG-3 parasitizing on Meloidogyne incognita eggs
62 schema:pagination 195
63 schema:productId N1e7f101961674b7a95c31b14b51d176f
64 N2fb652beac274057907afe5b46d48e8b
65 N3aec613806474d97876eea4944b6e3da
66 N4e2f986b7e7c4c15880211bb5e1c24a6
67 Ncf1d31954c3f42bfabca38b997e6d0fd
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048615046
69 https://doi.org/10.1007/s10295-008-0485-8
70 schema:sdDatePublished 2019-04-11T14:28
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher N6dfc023f92544722ba6bd21accce5688
73 schema:url http://link.springer.com/10.1007/s10295-008-0485-8
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N0c41e6c53acf47959d9b12420c29fc68 rdf:first sg:person.01144400066.28
78 rdf:rest N539216968fec484295ada8ff6742f8d6
79 N1e7f101961674b7a95c31b14b51d176f schema:name pubmed_id
80 schema:value 18936994
81 rdf:type schema:PropertyValue
82 N2fb652beac274057907afe5b46d48e8b schema:name dimensions_id
83 schema:value pub.1048615046
84 rdf:type schema:PropertyValue
85 N3aec613806474d97876eea4944b6e3da schema:name doi
86 schema:value 10.1007/s10295-008-0485-8
87 rdf:type schema:PropertyValue
88 N49285d3010dc4125b47801b3cdd51872 rdf:first sg:person.01244206253.55
89 rdf:rest Ne85390241928440190ae869236776a2b
90 N4e2f986b7e7c4c15880211bb5e1c24a6 schema:name readcube_id
91 schema:value e797d2853dfa7bddc0d2f827bd9594ad595c80af6c955f78f268ba8bc4de92f8
92 rdf:type schema:PropertyValue
93 N539216968fec484295ada8ff6742f8d6 rdf:first sg:person.01163670477.24
94 rdf:rest Nafc6ba04737b4b71a7d2979e926a9fa6
95 N53dfe7e86e2042ca954cf21c05ec7141 rdf:first sg:person.01061644453.35
96 rdf:rest N0c41e6c53acf47959d9b12420c29fc68
97 N60f7b13c59744ee391a2f6419b64b0e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Chitinases
99 rdf:type schema:DefinedTerm
100 N6b14505491c44078a41c77c133e23454 schema:volumeNumber 36
101 rdf:type schema:PublicationVolume
102 N6dfc023f92544722ba6bd21accce5688 schema:name Springer Nature - SN SciGraph project
103 rdf:type schema:Organization
104 N8969120284144f7bb4086653a453206d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Ovum
106 rdf:type schema:DefinedTerm
107 N91cf34ee2acd40b2a3d4c1e96ea403de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Animals
109 rdf:type schema:DefinedTerm
110 N948523dee22a496c8d1ed17162eb4cd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Molecular Sequence Data
112 rdf:type schema:DefinedTerm
113 Na55e9b6627bb4446865ef313de964949 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Biotechnology
115 rdf:type schema:DefinedTerm
116 Nafc6ba04737b4b71a7d2979e926a9fa6 rdf:first sg:person.01026766755.10
117 rdf:rest rdf:nil
118 Nc170067a2fac48fd93f53b6e246e51b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Host-Parasite Interactions
120 rdf:type schema:DefinedTerm
121 Nc66cfe524c52455c97c91c7a27ece576 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Amino Acid Sequence
123 rdf:type schema:DefinedTerm
124 Ncb264b0866754b629e163291cbffb85e schema:issueNumber 2
125 rdf:type schema:PublicationIssue
126 Ncb429c13ee9b4b44b478f3c7ccf74b93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Tylenchoidea
128 rdf:type schema:DefinedTerm
129 Ncf1d31954c3f42bfabca38b997e6d0fd schema:name nlm_unique_id
130 schema:value 9705544
131 rdf:type schema:PropertyValue
132 Nd299489c69b2480586fd08391f885821 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Hypocreales
134 rdf:type schema:DefinedTerm
135 Ne85390241928440190ae869236776a2b rdf:first sg:person.01334100633.19
136 rdf:rest N53dfe7e86e2042ca954cf21c05ec7141
137 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
138 schema:name Chemical Sciences
139 rdf:type schema:DefinedTerm
140 anzsrc-for:0302 schema:inDefinedTermSet anzsrc-for:
141 schema:name Inorganic Chemistry
142 rdf:type schema:DefinedTerm
143 sg:journal.1096599 schema:issn 1367-5435
144 1476-5535
145 schema:name Journal of Industrial Microbiology & Biotechnology
146 rdf:type schema:Periodical
147 sg:person.01026766755.10 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
148 schema:familyName Park
149 schema:givenName Ro-Dong
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026766755.10
151 rdf:type schema:Person
152 sg:person.01061644453.35 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
153 schema:familyName Kim
154 schema:givenName Young-Ju
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061644453.35
156 rdf:type schema:Person
157 sg:person.01144400066.28 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
158 schema:familyName Kim
159 schema:givenName Kil-Yong
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144400066.28
161 rdf:type schema:Person
162 sg:person.01163670477.24 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
163 schema:familyName Kim
164 schema:givenName Young-Cheol
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163670477.24
166 rdf:type schema:Person
167 sg:person.01244206253.55 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
168 schema:familyName Nguyen
169 schema:givenName Van-Nam
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244206253.55
171 rdf:type schema:Person
172 sg:person.01334100633.19 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
173 schema:familyName Oh
174 schema:givenName In-Jae
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334100633.19
176 rdf:type schema:Person
177 sg:pub.10.1007/s00253-003-1490-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016763631
178 https://doi.org/10.1007/s00253-003-1490-5
179 rdf:type schema:CreativeWork
180 sg:pub.10.1007/s00253-004-1877-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1035526387
181 https://doi.org/10.1007/s00253-004-1877-y
182 rdf:type schema:CreativeWork
183 sg:pub.10.1007/s00253-005-0183-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002750710
184 https://doi.org/10.1007/s00253-005-0183-7
185 rdf:type schema:CreativeWork
186 sg:pub.10.1007/s00284-005-0412-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025843459
187 https://doi.org/10.1007/s00284-005-0412-4
188 rdf:type schema:CreativeWork
189 sg:pub.10.1007/s00284-006-0078-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041235368
190 https://doi.org/10.1007/s00284-006-0078-6
191 rdf:type schema:CreativeWork
192 sg:pub.10.1007/s00284-007-9033-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046017344
193 https://doi.org/10.1007/s00284-007-9033-4
194 rdf:type schema:CreativeWork
195 sg:pub.10.1007/s00792-003-0338-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021178020
196 https://doi.org/10.1007/s00792-003-0338-3
197 rdf:type schema:CreativeWork
198 sg:pub.10.1007/s11274-004-4797-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028581289
199 https://doi.org/10.1007/s11274-004-4797-1
200 rdf:type schema:CreativeWork
201 sg:pub.10.1023/a:1008964324425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032143255
202 https://doi.org/10.1023/a:1008964324425
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
205 https://doi.org/10.1038/227680a0
206 rdf:type schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1077202108 schema:CreativeWork
208 https://doi.org/10.1006/abio.1993.1010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006799025
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1006/fgbi.1998.1111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049807053
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1006/fgbi.2001.1312 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039849553
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1006/jipa.1999.4843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005240131
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0003-2697(89)90653-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028872847
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0076-6879(90)82009-q schema:sameAs https://app.dimensions.ai/details/publication/pub.1045878957
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/b978-0-12-372180-8.50042-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004812051
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.bbapap.2005.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017784534
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.biocontrol.2004.07.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026985755
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.pep.2003.07.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041415575
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.resmic.2004.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007736406
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s0141-0229(00)00134-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034421903
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s0141-0229(00)00362-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040704567
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1017/s0031182000043158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013975928
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1042/bj2800309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002668698
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1080/09583150701668658 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034980465
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1110/ps.9.3.544 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045440855
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1111/j.1574-6976.1993.tb00004.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043775195
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1111/j.1742-4658.2005.04994.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031413968
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1111/j.1742-4658.2007.05955.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011744769
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1139/m69-122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032840353
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1139/w05-020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028135799
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1163/1568541041218004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034748522
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1201/9781420040821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1095907050
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1271/bbb1961.35.1154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020765446
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1271/bbb1961.51.1189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000960547
261 rdf:type schema:CreativeWork
262 https://doi.org/10.2225/vol4-issue3-fulltext-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069345679
263 rdf:type schema:CreativeWork
264 https://www.grid.ac/institutes/grid.14005.30 schema:alternateName Chonnam National University
265 schema:name Division of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University, 500-757, Gwangju, South Korea
266 Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, South Korea
267 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...