Acrylamide synthesis using agar entrapped cells of Rhodococcus rhodochrous PA-34 in a partitioned fed batch reactor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-11-10

AUTHORS

Jog Raj, Nitya Nand Sharma, Shreenath Prasad, Tek Chand Bhalla

ABSTRACT

The nitrile hydratase (Nhase) induced cells of Rhodococcus rhodochrous PA-34 catalyzed the conversion of acrylonitrile to acrylamide. The cells of R. rhodochrous PA-34 immobilized in 2% (w/v) agar (1.76 mg dcw/ml agar matrix) exhibited maximum Nhase activity (8.25 U/mg dcw) for conversion of acrylonitrile to acrylamide at 10°C in the reaction mixture containing 0.1 M potassium phosphate buffer (pH 7.5), 8% (w/v) acrylonitrile and immobilized cells equivalent to 1.12 mg dcw (dry cell weight) per ml. In a partitioned fed batch reaction at 10°C, using 1.12 g dcw immobilized cells in a final volume of 1 l, a total of 372 g of acrylonitrile was completely hydrated to acrylamide (498 g) in 24 h. From the above reaction mixture 87% acrylamide (432 g) was recovered through crystallization at 4°C. By recycling the immobilized biocatalyst (six times), a total of 2,115 g acrylamide was produced. More... »

PAGES

35

References to SciGraph publications

  • 1987-08. Continuous immobilized cell reactor for amide hydrolysis in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 1993-11. The superiority of the third-generation catalyst, Rhodococcus rhodochrous J1 nitrile hydratase, for industrial production of acrylamide in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1980. Bioconversions of nitriles and their applications in ADVANCES IN BIOCHEMICAL ENGINEERING, VOLUME 14
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10295-007-0263-z

    DOI

    http://dx.doi.org/10.1007/s10295-007-0263-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018688360

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17994258


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