Expression of a putative dioxygenase gene adjacent to an insertion mutation is involved in the short internodes of columnar apples ... View Full Text


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Article Info

DATE

2016-09-20

AUTHORS

Kazuma Okada, Masato Wada, Shigeki Moriya, Yuichi Katayose, Hiroko Fujisawa, Jianzhong Wu, Hiroyuki Kanamori, Kanako Kurita, Harumi Sasaki, Hiroshi Fujii, Shingo Terakami, Hiroshi Iwanami, Toshiya Yamamoto, Kazuyuki Abe

ABSTRACT

Determining the molecular mechanism of fruit tree architecture is important for tree management and fruit production. An apple mutant ‘McIntosh Wijcik’, which was discovered as a bud mutation from ‘McIntosh’, exhibits a columnar growth phenotype that is controlled by a single dominant gene, Co. In this study, the mutation and the Co gene were analyzed. Fine mapping narrowed the Co region to a 101 kb region. Sequence analysis of the Co region and the original wild-type co region identified an insertion mutation of an 8202 bp long terminal repeat (LTR) retroposon in the Co region. Segregation analysis using a DNA marker based on the insertion polymorphism showed that the LTR retroposon was closely associated with the columnar growth phenotype. RNA-seq and RT-PCR analysis identified a promising Co candidate gene (91071-gene) within the Co region that is specifically expressed in ‘McIntosh Wijcik’ but not in ‘McIntosh’. The 91071-gene was located approximately 16 kb downstream of the insertion mutation and is predicted to encode a 2-oxoglutarate-dependent dioxygenase involved in an unknown reaction. Overexpression of the 91071-gene in transgenic tobaccos and apples resulted in phenotypes with short internodes, like columnar apples. These data suggested that the 8202 bp retroposon insertion in ‘McIntosh Wijcik’ is associated with the short internodes of the columnar growth phenotype via upregulated expression of the adjacent 91071-gene. Furthermore, the DNA marker based on the insertion polymorphism could be useful for the marker-assisted selection of columnar apples. More... »

PAGES

1109-1126

References to SciGraph publications

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  • 2013-12-15. The columnar mutation (“Co gene”) of apple (Malus × domestica) is associated with an integration of a Gypsy-like retrotransposon in MOLECULAR BREEDING
  • 2012-11-03. Genetic and physical characterisation of the locus controlling columnar habit in apple (Malus × domestica Borkh.) in MOLECULAR BREEDING
  • 2002-11. The genome sequence and structure of rice chromosome 1 in NATURE
  • 2002-08. Apple has two orthologues of FLORICAULA/LEAFY involved in flowering in PLANT MOLECULAR BIOLOGY
  • 2012-12-18. How important are transposons for plant evolution? in NATURE REVIEWS GENETICS
  • 2005-09. Mapping Co, a gene controlling the columnar phenotype of apple, with molecular markers in EUPHYTICA
  • 2010-08-29. The genome of the domesticated apple (Malus × domestica Borkh.) in NATURE GENETICS
  • 2012-04-17. Fine genetic mapping of the Co locus controlling columnar growth habit in apple in MOLECULAR GENETICS AND GENOMICS
  • 2011-09-25. Identification of a functional transposon insertion in the maize domestication gene tb1 in NATURE GENETICS
  • 2013-01-10. Evaluation of the hormonal state of columnar apple trees (Malus x domestica) based on high throughput gene expression studies in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10265-016-0863-7

    DOI

    http://dx.doi.org/10.1007/s10265-016-0863-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040188253

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27650512


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