False positive of an immunochromatography kit for detection of norovirus in neonatal feces View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-02

AUTHORS

Takahiro Niizuma, Kaoru Obinata, Hiromi Ikari, Ayako Kamata, Tsubasa Lee, Keiji Kinoshita, Toshiaki Shimizu

ABSTRACT

Norovirus was detected in the feces from five neonates in the growing care unit by a rapid immunochromatography (ICG) kit. However, confirmation using reverse transcription polymerase chain reaction (RT-PCR), RT-loop-mediated isothermal amplification (RT-LAMP), and nested RT-PCR methods showed negative results from all the feces. In addition, the ICG test for the detection of norovirus was positive for four cases out of the 16 feces from other asymptomatic neonates/infants. Only one feces out of the four samples was positive by RT-LAMP. In this study, among the factors related to false positives with the norovirus ICG kit, there were no differences regarding the commencement of feeding, nutrition, and sample collection methods. Since the false positive rate of ICG in the diagnosis of norovirus infection in neonates and early infancy is high, ICG is not an appropriate method, and it is necessary to confirm the results using reliable methods like RT-PCR. More... »

PAGES

171-173

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10156-012-0439-y

DOI

http://dx.doi.org/10.1007/s10156-012-0439-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012577443

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22699298


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