BCG effects on telomerase activity in bladder cancer cell lines View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-06

AUTHORS

H. Saitoh, K. Mori, S. Kudoh, H. Itoh, N. Takahashi, T. Suzuki

ABSTRACT

BACKGROUND: Although intravesical bacillus Calmette-Guerin (BCG) therapy is very effective in the treatment of and prophylaxis against superficial bladder cancer, its exact mechanism of action is not clear. In this study, the effect of BCG on telomerase activity was examined in the T24 and J82 bladder cancer cell lines, the ACHN human renal cell carcinoma cell line, and the PC-3 human prostate cancer cell line. METHODS: T24 and J82 cells were cocultured with BCG for 5 days. Telomerase activity was measured by telomeric repeat amplification protocol. Cell-cycle phase was determined by FACS analysis. RESULTS: Telomerase activity in all cell lines provided high absorbance. Telomerase activity in BCG-treated T24 and J82 cells was significantly decreased when compared with that in the nontreated cells. On the other hand, telomerase activity did not change in ACHN and PC-3 cells after they had been cocultured with BCG. In BCG-treated T24 cells and J82 cells, apoptotic cells were markedly increased compared with those in the nontreated cells. CONCLUSION: These results suggest that the reduction of telomerase activity is related to the mechanism of BCG effects. Possible mechanisms to be considered are either that BCG inhibits telomerase first, or that it induces apoptosis and decreases telomerase activity as a result of this induction. More... »

PAGES

165-170

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s101470200024

DOI

http://dx.doi.org/10.1007/s101470200024

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005456878

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12109518


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adjuvants, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "BCG Vaccine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma, Renal Cell", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Prostatic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Telomerase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Urinary Bladder Neoplasms", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hirosaki University", 
          "id": "https://www.grid.ac/institutes/grid.257016.7", 
          "name": [
            "Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saitoh", 
        "givenName": "H.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hirosaki University", 
          "id": "https://www.grid.ac/institutes/grid.257016.7", 
          "name": [
            "Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mori", 
        "givenName": "K.", 
        "id": "sg:person.01106131643.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106131643.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hirosaki University", 
          "id": "https://www.grid.ac/institutes/grid.257016.7", 
          "name": [
            "Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kudoh", 
        "givenName": "S.", 
        "id": "sg:person.01154245043.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154245043.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hirosaki University", 
          "id": "https://www.grid.ac/institutes/grid.257016.7", 
          "name": [
            "Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Itoh", 
        "givenName": "H.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hirosaki University", 
          "id": "https://www.grid.ac/institutes/grid.257016.7", 
          "name": [
            "Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takahashi", 
        "givenName": "N.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hirosaki University", 
          "id": "https://www.grid.ac/institutes/grid.257016.7", 
          "name": [
            "Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suzuki", 
        "givenName": "T.", 
        "id": "sg:person.01336606643.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336606643.65"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2002-06", 
    "datePublishedReg": "2002-06-01", 
    "description": "BACKGROUND: Although intravesical bacillus Calmette-Guerin (BCG) therapy is very effective in the treatment of and prophylaxis against superficial bladder cancer, its exact mechanism of action is not clear. In this study, the effect of BCG on telomerase activity was examined in the T24 and J82 bladder cancer cell lines, the ACHN human renal cell carcinoma cell line, and the PC-3 human prostate cancer cell line.\nMETHODS: T24 and J82 cells were cocultured with BCG for 5 days. Telomerase activity was measured by telomeric repeat amplification protocol. Cell-cycle phase was determined by FACS analysis.\nRESULTS: Telomerase activity in all cell lines provided high absorbance. Telomerase activity in BCG-treated T24 and J82 cells was significantly decreased when compared with that in the nontreated cells. On the other hand, telomerase activity did not change in ACHN and PC-3 cells after they had been cocultured with BCG. In BCG-treated T24 cells and J82 cells, apoptotic cells were markedly increased compared with those in the nontreated cells.\nCONCLUSION: These results suggest that the reduction of telomerase activity is related to the mechanism of BCG effects. Possible mechanisms to be considered are either that BCG inhibits telomerase first, or that it induces apoptosis and decreases telomerase activity as a result of this induction.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s101470200024", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1312225", 
        "issn": [
          "1341-9625", 
          "1437-7772"
        ], 
        "name": "International Journal of Clinical Oncology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "BCG effects on telomerase activity in bladder cancer cell lines", 
    "pagination": "165-170", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1a00163ee72eafd21acce9d90232cb29ef684b5bca929d38c845f31b2623b248"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12109518"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9616295"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s101470200024"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005456878"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s101470200024", 
      "https://app.dimensions.ai/details/publication/pub.1005456878"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:24", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000480.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s101470200024"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s101470200024'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s101470200024'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s101470200024'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s101470200024'


 

This table displays all metadata directly associated to this object as RDF triples.

149 TRIPLES      20 PREDICATES      41 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s101470200024 schema:about N0044c5d6538642c3a059befc213081a4
2 N2196aa8d84ec415f891816e4df449075
3 N45412d1e441c41a5947e5fac26eff994
4 N4993b51d2a7148108f0c45527feebd26
5 N4e5318a21eb6470c89d683de70f6de5f
6 N6a583baf7901481f897fd45683806323
7 N6b275c4420a14f3ca56d71235368a835
8 N7ebbe69761994ab681359d951c07f016
9 N9d4deb601e444218bb22e35553ff5962
10 Nf434e791d77748788ffb9fdf569bb926
11 Nf56aa375f2a54d1082d2c7df4278342a
12 Nf75d1d692f364aa89cc03007427194ce
13 anzsrc-for:11
14 anzsrc-for:1112
15 schema:author Nb84ed43f556445c48ae1a5fcdb295e2c
16 schema:datePublished 2002-06
17 schema:datePublishedReg 2002-06-01
18 schema:description BACKGROUND: Although intravesical bacillus Calmette-Guerin (BCG) therapy is very effective in the treatment of and prophylaxis against superficial bladder cancer, its exact mechanism of action is not clear. In this study, the effect of BCG on telomerase activity was examined in the T24 and J82 bladder cancer cell lines, the ACHN human renal cell carcinoma cell line, and the PC-3 human prostate cancer cell line. METHODS: T24 and J82 cells were cocultured with BCG for 5 days. Telomerase activity was measured by telomeric repeat amplification protocol. Cell-cycle phase was determined by FACS analysis. RESULTS: Telomerase activity in all cell lines provided high absorbance. Telomerase activity in BCG-treated T24 and J82 cells was significantly decreased when compared with that in the nontreated cells. On the other hand, telomerase activity did not change in ACHN and PC-3 cells after they had been cocultured with BCG. In BCG-treated T24 cells and J82 cells, apoptotic cells were markedly increased compared with those in the nontreated cells. CONCLUSION: These results suggest that the reduction of telomerase activity is related to the mechanism of BCG effects. Possible mechanisms to be considered are either that BCG inhibits telomerase first, or that it induces apoptosis and decreases telomerase activity as a result of this induction.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N0f778e7054fc426fbca1046dfe52c2ca
23 N9809e7e031c7489ca18aeb5a7aee3b4f
24 sg:journal.1312225
25 schema:name BCG effects on telomerase activity in bladder cancer cell lines
26 schema:pagination 165-170
27 schema:productId N200c6807e75b446bbf84947a0280411a
28 N5acb1fdd064c4f4cbf5bc10e57abb497
29 N75f57f5a2f404e44b4ccffdc8d8681b5
30 N839b190644b2457c819a68de493ec722
31 Nf6546cb9bf954dbfa8b5836adbc2efd2
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005456878
33 https://doi.org/10.1007/s101470200024
34 schema:sdDatePublished 2019-04-10T22:24
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher Nb51ca8f2740245e18c7717e6651d8592
37 schema:url http://link.springer.com/10.1007/s101470200024
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N0044c5d6538642c3a059befc213081a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
42 schema:name BCG Vaccine
43 rdf:type schema:DefinedTerm
44 N033d0df2da794945b454065985965634 rdf:first sg:person.01336606643.65
45 rdf:rest rdf:nil
46 N0500e45208ef42d5b095afa43aaf126a schema:affiliation https://www.grid.ac/institutes/grid.257016.7
47 schema:familyName Itoh
48 schema:givenName H.
49 rdf:type schema:Person
50 N0f778e7054fc426fbca1046dfe52c2ca schema:issueNumber 3
51 rdf:type schema:PublicationIssue
52 N200c6807e75b446bbf84947a0280411a schema:name pubmed_id
53 schema:value 12109518
54 rdf:type schema:PropertyValue
55 N2196aa8d84ec415f891816e4df449075 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Tumor Cells, Cultured
57 rdf:type schema:DefinedTerm
58 N45412d1e441c41a5947e5fac26eff994 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name Humans
60 rdf:type schema:DefinedTerm
61 N4993b51d2a7148108f0c45527feebd26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Urinary Bladder Neoplasms
63 rdf:type schema:DefinedTerm
64 N4e5318a21eb6470c89d683de70f6de5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Cell Cycle
66 rdf:type schema:DefinedTerm
67 N5acb1fdd064c4f4cbf5bc10e57abb497 schema:name doi
68 schema:value 10.1007/s101470200024
69 rdf:type schema:PropertyValue
70 N6a583baf7901481f897fd45683806323 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Carcinoma, Renal Cell
72 rdf:type schema:DefinedTerm
73 N6b275c4420a14f3ca56d71235368a835 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Male
75 rdf:type schema:DefinedTerm
76 N6bd8da4b6233458a85afda90128870db rdf:first Nd5d2cbdd18a140fd97d5e7b0c03ad9e1
77 rdf:rest N033d0df2da794945b454065985965634
78 N75f57f5a2f404e44b4ccffdc8d8681b5 schema:name nlm_unique_id
79 schema:value 9616295
80 rdf:type schema:PropertyValue
81 N7ebbe69761994ab681359d951c07f016 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Adjuvants, Immunologic
83 rdf:type schema:DefinedTerm
84 N839b190644b2457c819a68de493ec722 schema:name dimensions_id
85 schema:value pub.1005456878
86 rdf:type schema:PropertyValue
87 N9809e7e031c7489ca18aeb5a7aee3b4f schema:volumeNumber 7
88 rdf:type schema:PublicationVolume
89 N9d4deb601e444218bb22e35553ff5962 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Cell Survival
91 rdf:type schema:DefinedTerm
92 Na7e41a2636934fd7aae6fb79eeaae7e3 rdf:first sg:person.01106131643.02
93 rdf:rest Ncb6d58a134f04903ac57f3d447d4ebc6
94 Nb51ca8f2740245e18c7717e6651d8592 schema:name Springer Nature - SN SciGraph project
95 rdf:type schema:Organization
96 Nb84ed43f556445c48ae1a5fcdb295e2c rdf:first Ne9366c64764a4e6ea8482a6fca13ae7d
97 rdf:rest Na7e41a2636934fd7aae6fb79eeaae7e3
98 Ncb298865aa6f486191f752f4a5736f51 rdf:first N0500e45208ef42d5b095afa43aaf126a
99 rdf:rest N6bd8da4b6233458a85afda90128870db
100 Ncb6d58a134f04903ac57f3d447d4ebc6 rdf:first sg:person.01154245043.07
101 rdf:rest Ncb298865aa6f486191f752f4a5736f51
102 Nd5d2cbdd18a140fd97d5e7b0c03ad9e1 schema:affiliation https://www.grid.ac/institutes/grid.257016.7
103 schema:familyName Takahashi
104 schema:givenName N.
105 rdf:type schema:Person
106 Ne9366c64764a4e6ea8482a6fca13ae7d schema:affiliation https://www.grid.ac/institutes/grid.257016.7
107 schema:familyName Saitoh
108 schema:givenName H.
109 rdf:type schema:Person
110 Nf434e791d77748788ffb9fdf569bb926 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Telomerase
112 rdf:type schema:DefinedTerm
113 Nf56aa375f2a54d1082d2c7df4278342a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Kidney Neoplasms
115 rdf:type schema:DefinedTerm
116 Nf6546cb9bf954dbfa8b5836adbc2efd2 schema:name readcube_id
117 schema:value 1a00163ee72eafd21acce9d90232cb29ef684b5bca929d38c845f31b2623b248
118 rdf:type schema:PropertyValue
119 Nf75d1d692f364aa89cc03007427194ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Prostatic Neoplasms
121 rdf:type schema:DefinedTerm
122 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
123 schema:name Medical and Health Sciences
124 rdf:type schema:DefinedTerm
125 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
126 schema:name Oncology and Carcinogenesis
127 rdf:type schema:DefinedTerm
128 sg:journal.1312225 schema:issn 1341-9625
129 1437-7772
130 schema:name International Journal of Clinical Oncology
131 rdf:type schema:Periodical
132 sg:person.01106131643.02 schema:affiliation https://www.grid.ac/institutes/grid.257016.7
133 schema:familyName Mori
134 schema:givenName K.
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106131643.02
136 rdf:type schema:Person
137 sg:person.01154245043.07 schema:affiliation https://www.grid.ac/institutes/grid.257016.7
138 schema:familyName Kudoh
139 schema:givenName S.
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154245043.07
141 rdf:type schema:Person
142 sg:person.01336606643.65 schema:affiliation https://www.grid.ac/institutes/grid.257016.7
143 schema:familyName Suzuki
144 schema:givenName T.
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336606643.65
146 rdf:type schema:Person
147 https://www.grid.ac/institutes/grid.257016.7 schema:alternateName Hirosaki University
148 schema:name Department of Urology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan Tel. +81-172-39-5091; Fax +81-172-39-5092 e-mail: moribio@cc.hirosaki-u.ac.jp, JP
149 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...